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Termini in Biological News

The structure of the Mre11 protein bound to DNA

...ch can image proteins and complexes in aqueous solution, closer to their natural state. "Like breaking a piece of wood into two parts, the severed termini of the DNA in a double-strand break have ends of varying shape," says Williams. "The high-resolution snapshots we obtained of Mre11 binding to differe...
Termini in Biological Technology

Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

Recombinant Enterokinase for highly specific digestion of purified proteins or proteins in crude extracts Ed Marsh * Johanna Claycomb * ChaoFeng Zheng * Denise Wyborski * John C. Bauer * Joseph A. Sorge * A.K. Bernardino * Peter Vaillancourt Stratagene Cloning Systems, Inc. Str...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

...verse direction. In the presence ofpyrophosphate and dsDNA, T4 DNA polymerase catalyzes the release ofdeoxynucleotide triphosphates (dNTPs) from the 3 termini of the DNA strands. The transphosphorylation reaction is catalyzed by the enzyme nucleoside diphosphate kinase (NDPK). In this reaction, the term...

The Bio-Rad Silver Stain, Rev E

...in, Clin Chem 30, 20842092 (1984) 76. Stefano JE, Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts, Cell 36, 145154 (1984) 77. Stephenson JR et al., Production and purification of murine monoclo...

Nuclease Protection Assays: The Basics

...ths. NPAs are also commonly used to precisely map mRNA termini and intron/exon junctions. RN...hat may comigrate on Northerns. NPAs can be used to map mRNA termini and intron/exon junctions. Multi...

siRNA Design: It's All in the Algorithm

...NA target site [9]. Going Beyond Terminal Tm In addition to G/C content at the termini of the siRNAs, traits that were found to be influential in defining optimal siRNA sequences include the T m...

More siRNA Vectors for RNA Interference

... of 3-6 uridines. The latter feature is especially important due to the apparent requirement that siRNAs have defined 3' termini that hybridize to an mRNA target (Elbashir et al. 2001b). Type III promoters have been selected for siRNA vector design ...

Dicer vs. RNase III for Preparation of siRNA Cocktails

...nucleotide overhangs with 5'-phosphate and 3'-hydroxyl termini (7-11). E. coli RNase III is involved in the maturat...eavage products range from ~12 to 15 bp in length with termini identical to those produced by Dicer (3). ...

Inducing RNAi with siRNA Cocktails Generated by RNase III

...and plasmid RNAs (6-9), digests long dsRNA to short duplex products ranging from ~12 to 15 bp in length with termini identical to those produced by Dicer (9). The average product length generated by RNase III digestion can be ...

GENE SILENCING WITH PCR PRODUCTS

...ouse U6 promoters. Each kit includes a DNA fragment containing one of the three promoters, PCR primers specific to the 5' and 3' termini of the SECs, purification reagents to prepare SECs for transfection, and siRNA template oligonucleotides that can be used to gen...

Avoid siRNA Design Altogether!

... 1230 bp dsRNA fragments with 2 to 3 nucleotide 3' overhangs, and 5'-phosphate and 3'-hydroxyl termini. The termini and overhangs of RNase III cleavage products are thus the same as those produced by Dicer in the in vivo RNAi ...
Termini in Biological Products

Apo-BrdU Apoptosis Detection Kit from eBioscience

Description:...laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with bromolated deoxyuridine triphosphate nucleotid...
Company:eBioscience

Apo-Direct Apoptosis Detection Kit from eBioscience

Description:...laddering on agarose gels during electrophoresis. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini in the DNA. This property can be used to identify apoptotic cells by labeling the 3'-hydroxyl ends with directly conjugated fluorescein- deoxyuridine ...
Company:eBioscience

S1 Nuclease from Promega

Description:...DNA:RNA or RNA:RNA) are resistant to degradation except with extremely high concentrations of enzyme (1). The enzyme is used to remove single-stranded termini from double-stranded DNA (2), for selective cleavage of single-stranded DNA and for mapping RNA transcripts (3)....
Company:Promega

Ligase, T4 DNA from USB Corp.

Description:... clone of T4 DNA Ligase. Description: T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphoryl and 3'-hydroxyl termini in duplex DNA or RNA. It repairs single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids and will join both blunt-end and cohesive-end fragments o...
Company:USB Corp.

Tfi DNA Ligase, 2,000U, thermostable from Bioneer, Inc.

Description:... Tfi DNA Ligase catalyzes the formation of a phosphodiester bonds between adjacent 3-hydroxyl and 5-phosphate termini in nicked duplex DNA molecules or between oligonucleotides which are in duplex with a complementary strand at 45 - 65?. Tfi DNA Ligase is stable and a...
Company:Bioneer, Inc.
Termini in Biological Dictionary

Phosphatase

... - updated daily ... Structure Of Putative Phosphatase From Streptococcus Mutans ... Since phosphatase -treated fragments lack the 5' phosphoryl termini required by ... Which phosphatase (CIP, Antarctic or SAP) should I use? ... Cyclin-dependent kinase inhibitor 3 (CDK2-associated dual specifici...

Nuclease

...e -free ... The nuclease as a model for protein folding, Molec. Cell. Biochem., 23, ... Nuclease BAL-31 exonuclease degrades both 3' and 5' termini of duplex DNA ... Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment? ... Micrococcal nuclease is derived fro...

Inverse PCR

...ction that is used to amplify DNA with only one known sequence. One limitation of conventional PCR is that it requires primers complementary to both termini of the target DNA, but... ... Inverse PCR or ... Inverse polymerase chain reaction is a variant of PCR , and is used ... The inverse PC...

DNA ligase

... of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and ... Cata... of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends. ... used DNA ligase is der...

Dicer

...as ... Dicer , an enzyme that can produce putative guide RNAs, is a member of the RNase ... Human Dicer preferentially cleaves dsRNAs at their termini without a requirement ... siRNAs generated by recombinant human Dicer induce specific and significant but ... Peas and Corn ... This food di...

Concatemer

...or concatemer ... Search completed in 0. ... In this process, only one of the two genomic ends are formed on the concatemer . ... The short arm termini on concatemer ends are then free to encounter additional ... The mechanism for generating concatemer -ISI is not fully understood. ... One possi...

CDNA clone

... a previously uncharacterized open ... This would have to be tested by cloning and sequencing the corresponding cDNA . ... 4. Repair of cDNA termini and ligation of adaptor oligos; clone into vector. ... If after isolating a cDNA clone from this library, you discover that the insert ... ...

Carboxyl Terminus

...hat the carboxyl terminus of ClC-1 is not necessary for ... Carboxyl termini fold independently and modify individual protopores of the ... Within t...sage examples and terminus quotes. ... the negatively charged carboxyl termini of the rhinovirus coat ... carboxyl terminus undergoes conformation...

C Terminus

... terminus ? Read answer... Cleavage of the C termini by limited proteolysis with subtilisin enhances...e: $100. ... ... the Guest Book for James C . termini , leave condolences, send flowers, or ... James C . termini This Guest Book has been kept online until 7/27/2...

Amino Terminus

...ce from N- to C- terminus . Cytoskeleton articles relating to ' amino termini ' ... undergo a unique post-translational modification of the amino te...ceptor, and the amino terminus conveys a hydropho ... at their amino termini ; this modi ... The amino terminus is clearly impor- tant for interacti...
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