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Terminators in Medical Technology

Genelabs Technologies Announces Presentation of Data on Non-Nucleoside HCV Polymerase Inhibitor at 20th International Conference on Antiviral Research Meeting

... these data suggest that combinations of this type of non-nucleoside agent with interferon or other likely therapeutic drugs, such as nucleoside chain terminators or protease inhibitors, may be feasible." About Genelabs Technologies Genelabs Technologies, Inc. is a biopharmaceutical company focused on the di...
Terminators in Biological News

NHGRI seeks DNA sequencing technologies fit for routine laboratory and medical use

...process. Jingyue Ju, Ph.D., Columbia University, New York $950,000 (2 years) DNA Sequencing with Reversible dNTP and Cleavable Fluorescent ddNTP terminators This team will develop a hybrid strategy that uses a mixture of chemically modified DNA constituents called nucleotides, along with new methods to ...
Terminators in Biological Technology

Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, ...

SeqWright to Invest in LaserGen's Novel Lightning Terminators(TM)

...lans to commercialize its novel reversible terminator chemistry, Lightning terminators (TM), for next-generation sequencing applications. The company anticipates ...stated, Lightning Terminators(TM) are better than nature. All reversible terminators required a cleavage step to remove the terminating and fluorescent dye grou...

Semi Preparative Purification of Proteins

...cases, it may be necessary to remove interfering and abundant proteins, such as albumin, IgG, IgA, transferrin, haptoglobin, and antitrypsin and dye terminators from human serum sample matrices. This may be performed by the use of Multiple Affinity Removal Systems or by the use of Spin columns. These system...

A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase

... background artifacts, usually making sequence interpretation difficult. These artifacts can be avoided by the use of [(a-33P] labeled ddNTPs. Labeled terminators prevent the visualization of sequencing artifacts because only specifically-terminated primer extension products are labeled. The Thermo Seq...

Comparison of Commercial Dye Terminator Removal Kits, Rev A

...purity. Samples must be free of unincorporated dye terminators and other contaminants, including salt....e accuracy of a read. Unincorporated dye terminators that are not removed from a sample prior ... to completely remove unincorporated dye terminators (Table 2). Princeton Separations Centri...

CleanSEQ on the ABI 3100

... CleanSEQ with water elution allow extensive dilution of BigDye terminators without affecting Phred20 read lengths (data not shown). ...EQ and water elution allow increased dilution of BigDye terminators resulting in substantial cost savings. Injection of samples con...

Automated Fluorescent-Tag Cycle Sequencing

... Cycle sequencing, using fluorescent dye-tagged terminators or primers, has become the standard protocol for... not incorporate the dideoxy nucleotide reaction terminators at a uniform rate between sample wells. Incorporat...fy lanes and detect the fluorescent-dye terminators within each lane. The lane tracking was...
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