Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators
Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, ...
SeqWright to Invest in LaserGen's Novel Lightning Terminators(TM)
...lans to commercialize its novel reversible terminator chemistry, Lightning terminators
(TM), for next-generation sequencing applications. The company anticipates ...stated, Lightning Terminators(TM) are better than nature. All reversible terminators
required a cleavage step to remove the terminating and fluorescent dye grou...
Semi Preparative Purification of Proteins
...cases, it may be necessary to remove interfering and abundant proteins, such as albumin, IgG, IgA, transferrin, haptoglobin, and antitrypsin and dye terminators
from human serum sample matrices. This may be performed by the use of Multiple Affinity Removal Systems or by the use of Spin columns. These system...
A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase
... background artifacts, usually making sequence interpretation difficult. These artifacts can be avoided by the use of [(a-33P] labeled ddNTPs. Labeled terminators
prevent the visualization of sequencing artifacts because only specifically-terminated primer extension products are labeled.
The Thermo Seq...
Comparison of Commercial Dye Terminator Removal Kits, Rev A
...purity. Samples must be free of unincorporated dye terminators
and other contaminants, including salt....e accuracy of a read.
Unincorporated dye terminators
that are not removed from a sample prior
to completely remove unincorporated dye terminators
Princeton Separations Centri...
CleanSEQ on the ABI 3100
... CleanSEQ with water elution allow extensive
dilution of BigDye terminators
Phred20 read lengths (data not shown).
water elution allow increased dilution of BigDye
resulting in substantial cost savings.
Injection of samples con...
Automated Fluorescent-Tag Cycle Sequencing
Cycle sequencing, using fluorescent dye-tagged terminators
or primers, has
become the standard protocol for... not incorporate the dideoxy nucleotide
at a uniform rate between sample wells. Incorporat...fy lanes and
detect the fluorescent-dye terminators
within each lane. The lane tracking