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TaqMaster in Biological Technology

The use of TaqMaster PCR Enhancer

Guy Adami, Department of Oral Medicine, University of Illinois in Chicago, USA Vincent Prezioso, Eppendorf Scientific, Inc., Westbury, NY, USA Introduction Transgenic mice carr...

The TripleMaster PCR System for three different amplifications

...ts either in combination with only 2 % DMSO or with only Eppendorf taqmaster (1x) see Fig. 3. ... applicational problems) adding low concentrations of DMSO or Eppendorf taqmaster is sufficient to obtain very good results. Since TaqMaster, unlike...

The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA

... pmol of each primer, 2.5 mol of each deoxyribonucleoside triphosphate, 300 g bovine serum albumin (BSA), 1x reaction buffer, 1x taqmaster PCR enhancer and 1U MasterTaq DNA Polymerase were adjusted to a final volume of 100 l with sterile water. From 80 ng to 500 ng o...

Enhancing PCR* Efficiency with MasterTaq

... failed. The application of the PCR enhancer taqmaster is highly recommended for solving probl..., 1 unit Taq DNA Polymerase and 10 l 5x taqmaster PCR enhancer in a total volume of 50 l ... These results demonstrate the positive effect of taqmaster on thermal stability and processivity o...

Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq

...rf MasterTaq polymerase kit. Varying the amount of taqmaster thermostabilizer included in the PCR re... obtained from N. oceani. The addition of 2.5 l of taqmaster gave the most favorable results (Lane 4...s were visible at all concentrations of taqmaster (Lanes 3 and 5). The additional bands s...

TripleMaster PCR System

...estabilizing co-solvents substantially reduces the PCR fidelity and requires a re-optimization of the annealing temperature. The taqmaster PCR Enhancer, available separately, can be used to stabilize the enzyme for elevated denaturation temperatures (up to 98C) durin...

TripleMaster PCR System

...estabilizing co-solvents substantially reduces the PCR fidelity and requires a re-optimization of the annealing temperature. The taqmaster PCR Enhancer, available separately, can be used to stabilize the enzyme for elevated denaturation temperatures (up to 98C) durin...
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