TOPO

First look at an enzyme target for antibacterial and cancer drugs

...he chromosome during cell division. Preventing topo II from disentangling a cells DNA is fatal to the cell, which is why drugs that target topo II serve as agents against bacterial infections an...rms of cancer. This first ever structural image of topo II should help in the development of future antiba...

TOPO in Biological News

The right drug at the right time

... this process was trioctylphosphine oxide, or TOPO, which costs more than $150 per liter. Later, other scientists introduced a new recipe by replacing topo with a mixture of ODE and oleic acid. Wong said the CBEN research team, which included CBEN Director Vicki Colvin, professor of chemistry, and Niko...

Method slashes quantum dot costs by 80 percent

... this process was trioctylphosphine oxide, or TOPO, which costs more than $150 per liter. Later, other scientists introduced a new recipe by replacing topo with a mixture of ODE and oleic acid. Wong said the CBEN research team, which included CBEN Director Vicki Colvin, professor of chemistry, and Niko...

TOPO in Biological Technology

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...sit www.arctur.com for the complete Protocol #3. VI. IN SITU HYBRIDIZATION ◊ Gene-specific probes ◊ pCRII topo cloning kit (Invitrogen Cat. #K460001) ◊ p 33 -labeled dUTP (Amersham) ◊ Cold formaldehyde (Sigma) ◊ ...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

...esigned by the authors and synthesized by Life Technologies. The topo TA cloning kit from Invitrogen was used for cloning. The agarose...ks of Roche Molecular Systems, Inc. One Shot, pCR, Platinum, and topo are trademarks of Invitrogen Corp. RNAzol is a trademark of Cinn...

TOPO in Biological Products

Zero Blunt TOPO PCR Cloning Kit for Sequencing with One Shot TOP10 Electrocomp E. coli from Invitrogen

Description: The Zero Blunt TOPO PCR Cloning Kit for Sequencing is designed for cloning and subsequent sequencing of blunt-end PCR products. The pCR4Blunt-TOPO vector provides 5-minute TOPO Cloning and greater than or equal to95% recombinants (1). Primer sites are loca...
Company:Invitrogen

Zero Blunt TOPO PCR Cloning Kit for Sequencing with One Shot MAX Efficiency DH5Alpha-T1R E. coli from Invitrogen

Description: The Zero Blunt TOPO PCR Cloning Kit for Sequencing is designed for cloning and subsequent sequencing of blunt-end PCR products. The pCR4Blunt-TOPO vector provides 5-minute TOPO Cloning and greater than or equal to95% recombinants (1). Primer sites are loca...
Company:Invitrogen

Zero Blunt TOPO PCR Cloning Kit for Sequencing with One Shot TOP10 Electrocomp E. coli from Invitrogen

Description: The Zero Blunt TOPO PCR Cloning Kit for Sequencing is designed for cloning and subsequent sequencing of blunt-end PCR products. The pCR4Blunt-TOPO vector provides 5-minute TOPO Cloning and greater than or equal to95% recombinants (1). Primer sites are loca...
Company:Invitrogen

GeneRacer Kit with SuperScript II RT and Zero Blunt TOPO PCR Cloning kit for Sequencing from Invitrogen

Description: GeneRacer is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5' and 3' cDNA ends. With the GeneRacer Kit you can:...
Company:Invitrogen

GeneRacer Kit with AMV RT and TOPO TA Cloning kit for Sequencing from Invitrogen

Description: GeneRacer is an advanced RACE (rapid amplification of cDNA ends) technique that improves the efficiency of amplifying full-length 5' and 3' cDNA ends. With the GeneRacer Kit you can:...
Company:Invitrogen

HTP Zero Blunt TOPO PCR Cloning Kit for Sequencing MultiShot E. coli from Invitrogen

Description: TOPO Cloning is the most effective technology available for cloning PCR products. TOPO TA Cloning and Zero Blunt TOPO vectors are supplied linearized and activated with topoisomerase I. This enables 5-minute, bench-top ligation and yields >=95% recombi...
Company:Invitrogen

HTP Zero Blunt TOPO PCR Cloning Kit for Sequencing MultiShot StripWell E. coli from Invitrogen

Description: TOPO Cloning is the most effective technology available for cloning PCR products. TOPO TA Cloning and Zero Blunt TOPO vectors are supplied linearized and activated with topoisomerase I. This enables 5-minute, bench-top ligation and yields >=95% recombi...
Company:Invitrogen

Zero Blunt TOPO PCR Cloning Kit with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description: The Zero Blunt TOPO PCR Cloning Kit offers the fastest and easiest method for high-efficiency (>=95%) cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kit includes the linearized and topoisomerase I-activated pCR-Blunt II-TOPO vector for 5-minute bench-top...
Company:Invitrogen

Zero Blunt TOPO Kit for Sequencing with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description: The TOPO Cloning Kits for Sequencing allow 5-minute cloning and subsequent sequencing of PCR products. The kits contain TOPO Cloning vectors with primer sites located as close as 33 base pairs from the PCR product insertion site. This minimizes the amount of vector DNA that needs to be read before ...
Company:Invitrogen

Zero Blunt TOPO PCR Cloning Kit with One Shot TOP10 Chemically Competent E. coli from Invitrogen

Description: The Zero Blunt TOPO PCR Cloning Kit offers the fastest and easiest method for high-efficiency (greater than or equal to95%) cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kit includes the linearized and topoisom...
Company:Invitrogen

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TOPO in Biological Dictionary

Subcloning

...verview of the subcloning procedure. ... Subcloning PCR Products the topo Way. The Scientist 1998, 12 ... I promptly borrowed his topo TA Cloning kit and set about subcloning those pesky PCR products. ... ...

Gyrase

...vely in the ... In addition to gyrase , many bacteria contain another topo IIA named topo IV. ... The CTD of gyrase is indeed responsible for the positive wrapping...