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Supercoiled in Medical News

Penn Researchers Throw Light on the Structure Of Smallpox Virus Protein

...viral topoisomerase enzyme and the closely related human enzyme that functions in the nucleus of all human cells is that the viral enzyme only relaxes supercoiled DNA when it binds to specific DNA sequences. The structure of the poxvirus topoisomerase-DNA complex provides some important clues about how this reco...

Structure of DNA-modifying enzyme In Small Pox Determined

...viral topoisomerase enzyme and the closely related human enzyme that functions in the nucleus of all human cells is that the viral enzyme only relaxes supercoiled DNA when it binds to specific DNA sequences. The structure of the poxvirus topoisomerase-DNA complex provides some important clues about how this reco...
Supercoiled in Biological News

Bioclocks work by controlling chromosome coiling

...s in cyanobacteria are organized in circular molecules of DNA. In their relaxed state, they form a single loop. But, within the cell, they are usually supercoiled into a series of small helical loops. There are even two families of special enzymes, called gyrases and topoisomerases, whose function is coiling and...
Supercoiled in Biological Technology

Transgenic Zebrafish by Electroporation

...n, it is a possibility. Future experiments are planned using gel-purified linear fragments of various constructs to try to avoid supercoiled plasmid carryover. Also, there is great variability in the amount of signal seen from one positive animal to the next....

Electroporation of Yeast Artificial Chromosomes

...reported for the transformation of Saccharomyces cerevisiae with supercoiled plasmids. 3-6 In an attempt to improve the currently used YAC c...proximately 700 transformants / g of DNA. Transformations with a supercoiled 5 kb plasmid yielded up to 1.6 x 10 4 transformants / g of DNA....

Transformation of Nematodes With the Helios Gene Gun

... 100 l of 0.05 M spermidine (Sigma), and sonicate 3 times for 3 seconds in an Eppendorf tube (1.5 ml). 2. Add 200 l of purified supercoiled plasmid DNA to the carrier suspension (in a total volume of 100 l). It is possible to use more than one plasmid simultaneously. ...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

... involving most cell types (J. Sanford, pers. comm.). Both gold and tungsten microparticles were prepared, and coated with supercoiled plasmid essentially as described by Sanford, et al. (1992; see Appendices 1 and 2). Microparticles were dried onto macrocarriers ...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

... Electro-Competent E. coli JS5 cells and supercoiled pUC18 plasmid DNA (10 pg/l) were obtain...lasmid was prepared by digestion of the supercoiled form with EcoRI restriction enzyme (New...formation efficiency as did the control supercoiled pUC18 plasmid (Figure 1). Glycogen was ...

Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments

...plasmid size (kb). supercoiled and relaxed episomes were electroporated with equa...ificantly higher. In addition, circular supercoiled DNA molecules are physically quite stable and easy... 11. Leach, D., and Lindsey, J., In vivo loss of supercoiled DNA carrying a palindromic sequence, Mo...

PCR Grade Water

... Absence of nicking activity: 1 g supercoiled pBR322 DNA is incubated with vari-ous amounts of Water, PCR ...CR grade shows no detectable alteration or relaxation of the supercoiled DNA. Absence of ribunocleases: ...

Transfection Reagent Selector Kit Handbook

...sient transfection is most efficient with supercoiled plasmid DNA. In stable transfection, linear ...ults in lower DNA uptake by the cells, relative to supercoiled DNA, but yields optimal integration of DN...sed. Transient transfection is most efficient when supercoiled plasmid DNA is used. ...

Efficient Recovery of Ultrapure Plasmid DNA

...arkedly better than, those obtained with other kits on the market (Fig. 2). More than 95% of the extracted plasmid DNA is in the supercoiled form. Since the purity of the plasmid DNA is similar to DNA purified on a CsCI density gradient, it can be used directly for dow...

Efficient Transfection of Neurospora Crassa

...Procedure The suspension was transferred in 40 µl aliquots to two Eppendorf 2 ml tubes on ice. In Tube #1, 1 µg of supercoiled DNA (plasmid pCSN44) was added. The selection marker on this plasmid was for hygromycin B resistance [4] . In Tube #2, an equal...
Supercoiled in Biological Products

Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)

Description:... Ideal for relax positively and negatively supercoiled DNA in presence of EDTA Applications Studying the effects of supercoiling on transcription in vitro Studying chromatin reconstitution in vit...
Company:Molecular Cloning Laboratories (MCLAB)

Supercoiled DNA Ladder from Invitrogen

Description:... supercoiled DNA Ladder is suitable for sizing supercoiled DNA from 2 to 16 kb by agarose gel electrophoresis (1). The bands can be vi...
Company:Invitrogen

Human Topoisomerase II, Alpha from USB Corp.

Description:...ism, the enzyme can relax negatively or positively supercoiled DNA, as well as catenate/decatenate or knot/unknot... required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 plasmid DNA in 30 minutes at 37C under the ... Applications: *Relaxing negatively or positively supercoiled DNA. *Catenating or decatenating DNA. *Knottin...
Company:USB Corp.

Calf Topoisomerase I from USB Corp.

Description:...isomerase I relaxes both positively and negatively supercoiled DNA by a transient breakage and rejoining of phosphodiester bonds (1,2) . Relaxing supercoiled DNA with Topoisomerase I allows molecules to be di...nzyme required to catalyze the conversion of 0.5 g supercoiled pBR322 DNA to a relaxed state in 30 min at 37C. ...
Company:USB Corp.

Human Topoisomerase II, Alpha from USB Corp.

Description:...ism, the enzyme can relax negatively or positively supercoiled DNA, as well as catenate/decatenate or knot/unknot... required to fully relax 0.3 g (5nM) of negatively supercoiled pBR322 plasmid DNA in 30 minutes at 37C under the ... Applications: *Relaxing negatively or positively supercoiled DNA. *Catenating or decatenating DNA. *Knottin...
Company:USB Corp.

Human Topoisomerase I Wild Type from Calbiochem

Description:...single-copy gene. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and ...rotein. 0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction. Purity: ≥95% by SDS-PAGE . M.W. 85,000...
Company:Calbiochem

Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies

Description:...um ion may increase activity. The protein has a molecular weight of 32000. Unit Definition: One unit of Vaccinia Topoisomerase I will convert 1 m g of supercoiled DNA (Form 1) to relaxed closed circular DNA (Form II) in 1 hour at 37°C in 50 mM Tris-acetate, pH 7.5, 0.1 mM EDTA, 2.5 mM MgCl 2 and 100 mM Na...
Company:EPICENTRE Biotechnologies

Vaccinia DNA Topoisomerase I from EPICENTRE Biotechnologies

Description:...um ion may increase activity. The protein has a molecular weight of 32000. Unit Definition: One unit of Vaccinia Topoisomerase I will convert 1 m g of supercoiled DNA (Form 1) to relaxed closed circular DNA (Form II) in 1 hour at 37°C in 50 mM Tris-acetate, pH 7.5, 0.1 mM EDTA, 2.5 mM MgCl 2 and 100 mM Na...
Company:EPICENTRE Biotechnologies
Supercoiled in Biological Definition

DNA

...th 6.4 Different helix geometries 6.4.1 supercoiled DNA 6.4.2 Conditions for formation of A and Z h... base exerts on its neighbors in the chain. supercoiled DNA The B form of the DNA helix twists 360 per 1...ed to, respectively, as positively or negatively " supercoiled ". DNA in vivo is typically negatively supercoil...
Supercoiled in Biological Dictionary

Supercoil

...coil Slide at NexTag.com. Find Great Bargains on Sporting Goods, Toys, Collectibles, Car Parts, Food, and the Good Things in Life. DNA supercoil supercoiled structure of circular DNA molecules with low writhe. ... The noun form " supercoil " is rarely used in the ... What is a supercoil ? ... Shop for ...

Relaxed DNA

...one residual ... All topoisomerases can relax supercoiled DNA , and DNA gyrase, present in ... the inter-conversion of relaxed and supercoiled DNA , is not readily monitored. ... ... way to...s essential for the fundamental biological ... The supercoiled and relaxed DNA bands are shown. ... overwo...

Minicircles

...om the ... Finally, the progeny minicircles , still containing at least one nick or gap, are ... In Cryptobia helicis, these minicircles are supercoiled and non-catenated (13) ... Its minicircles encode classical guide RNA genes (14), which serve for editing ... posed of thousands of catenated mi...

DNA gyrase

...e ... II (called gyrase in bacteria) primarily ... DNA gyrase /topoisomerase IV, subunit A, C-terminal beta-pinwheel ... Gyrase also relaxes supercoiled DNA in an ATP-independent manner (14) ... In the domain of Archaea, TopoVI is often present and DNA gyrase is occasionally found (6) ... .....
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