SuperScript

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...74; RiboAmp RNA amplification kit (Arcturus, Cat. # KIT0203) ◊ superscript II reverse transcriptase (Invitrogen Life Technologies, Inc., Carlsbad, CA,... 8. Synthesize cDNA from amplified RNA with random hexamer primers and superscript II reverse transcriptase to confirm expression of cell type-specific gene t...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...ers and probes (Biosearch Technologies, Cat. # (see Table I) ◊ superscript II reverse transcriptase enzyme (Invitrogen, Cat. #18064-014) ◊...r (50mM Tris-HCl pH 8.3, 75mM KCl, 3mM MgCl2), 5mM DTT, 0.3mM dNTP mix, 20U superscript II reverse transcriptase enzyme, 2.5U Taq DNA polymerase, 40U RnaseOut RNas...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

...the manufacturer. First-strand cDNAs were synthesized from equal amounts of total RNA (1 g/reaction) using oligo(dT) primers and superscript II reverse transcriptase (Invitrogen) according to the manufacturers instructions. Gene-specific primers (GSPs) were designed to ...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

...lets were washed with 70% ethanol, dried, and resuspended in TE buffer. cDNA was prepared from the 2.3 kb mRNA supplied with the superscript plasmid system, according to instructions from the manufacturer (LTI). The cDNA was constructed with SalI and NotI adaptors. The...

RNA Amplification Just Got Better: MessageAmp II

...was performed with 100 U of either ArrayScript RT, superscript II (SSII), superscript III (SSIII), or wild type (wt) M-MLV RT in a 20 ...mparison of ArrayScript RT (AS; 200 U), superscript II (SSII; 200 U); wild type M-MLV RT (200 U), ...

HIGH SENSITIVITY qRT-PCR

... More sensitive than superscript II and other RNase H MMLV reverse trans...m several manufacturers, with superscript II (Invitrogen) being the most widely used. ...R detection sensitivity using superscript II MMLV RNase H RT to ampli...

ArrayScript RT: Maximize Yields of Full-length cDNA

...was performed with 100 U of either ArrayScript RT, superscript II (SSII), superscript III (SSIII), or wild type (wt) M-MLV RT in a 20 ...mparison of ArrayScript RT (AS; 200 U), superscript II (SSII; 200 U); wild type M-MLV RT (200 ...

C. therm. Polymerase One-Step RT-PCR System

...ure. Positive Controls: 1 ng of a 997 bp beta-actin fragment from mouse. Lane 1: C. therm. polymerase/Taq for 30 min at 60C. Lane 2: superscript II/Taq for 50 min at 42C. Lane 3: AMV(Promega)/Taq for 60 min at 42C. Result: C. therm works on templates where other systems fail. ...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

...dATP, 900 M dGTP, 900 M dTTP, 230 M dCTP, 230 M Cy3- or Cy5-dCTP (APBiotech, UK), 20 U of RNasin (Promega), and 200 U superscript II reverse transcriptase (Life Technologies). The reaction was incubated at 42C for 2 hours and heated at 100C for 5 minutes. RN...

An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat

... labeling, meaning the incorporation of Cy3-dCTP or Cy5-dCTP during cDNA synthesis, was subsequently performed. 100 g total RNA, superscript II Reverse Transcriptase (Invitrogen) and gene-specific primer were used for this reaction. In order to remove the RNA at the en...

SuperScript in Biological Dictionary

RT-PCR

...cted in conjunction ... Reverse Transcriptase- PCR ... The following is a great RT - PCR protocol I obtained from David Klein at NIH. ... 2.0 ul superscript II RT (200 U/ul)(Gibco BRL ... The latest PCR platforms, fluorescent chemistries, validation software, data ... a comprehensive overview of the...