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Subcloning in Biological Technology

Successful PCR amplification and subcloning of a GC-rich DNA fragment

Petra Majlingov, Paolo Paroncini, Silvia Svegliati, Michele Luchetti, and Armando Gabrielli Laboratorio di Medicina Molecolare, Istituto di Clinica Medica, Polo Didattico Scientifico, Ancona, Italy The formati...

Electrotransformation of E. coli With M13 DNA

... Introduction M13 vectors have been used extensively for subcloning mainly because the abundant, easily isolated single-stranded for...is strain. There is good motivation for using X-Gal and IPTG when subcloning into the M13 mp series vectors because the blue/white color sele...

Five Ways to Produce siRNAs

...App=145&fkProdGrp=287">pSEC vectors , which are pre-linearized with the same restriction enzymes and are designed especially for subcloning SECs, are available separately. These ready-to-ligate vectors include a hygromycin, neomycin or puromycin resistance gene to per...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

... involved [2] and is therefore usually circumvented by subcloning. Because of the absence of appropriate restriction sites in the DNA fragments, subcloning into another suitable vector and subsequent ligation of the two fragments in this construct was impossible in our case. A standard ligation prot...

Mammalian Expression Vectors with Interchangeable Selectable Markers

...iciently exchange drug-resistance elements without subcloning into such vectors. Cre-Mediated Recombinati...te conventional restriction digestion and ligation subcloning procedures, site-specific recombination is used...ct to be used for multiple applications without subcloning the gene of interest. This system features the ...

A New Lambda Vector for Mammalian Expression

...the convenience of plasmid libraries for functional studies. This elegant procedure requires very little hands-on time and eliminates the need for subcloning procedures. High-Level Expression in Mammalian Cells Figure 1 Strata...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

...us and time-consuming, requiring either single-stranded DNA templates 1 , subcloning into specialized vectors 2 , plasmid vectors containing unique restrictio... place in virtually any double-stranded plasmid, eliminating the need for subcloning into M13-based bacteriophage vectors and ssDNA rescue. In addition, the k...
Subcloning in Biological Products

TOPO Shotgun Subcloning Kit from Invitrogen

Description: ...
Company:Invitrogen

MaV203 Competent Yeast Cells Subcloning Scale from Invitrogen

Description: MaV203 Competent Yeast Cells are designed for use with the ProQuest Two-Hybrid System (Figure 1). S. cerevisiae strain MaV203 contains deletions in the endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems. The strain also has leu2 and trp1 mutations for selection of ProQuest b...
Company:Invitrogen

Bacterial system service from Abgent

Description:... Services include subcloning into an expression vector. Tagged protein will be expressed in small scale for testing yield and solubility and then in 1L culture for large scale aff...
Company:Abgent

Baculovirus system service from Abgent

Description:... Services include subcloning of gene in baculoviral expression vector, transfection of insect cells, isolation and plaque purification of ten clones, generation of 0.5L high-titer...
Company:Abgent

Hybridoma Development from QED Bioscience Inc.

Description:...Supernatant samples available to the client for testing in their system for subcloning selection. Antibody is produced from 5 clones selected by the client; ...ystems. Up to 25 antibody-positive cultures will be carried through the subcloning and analysis phase. 5 clones may be selected for Antibody Production; a...
Company:QED Bioscience Inc.
Subcloning in Biological Dictionary

Subcloning

In molecular biology, subcloning is a technique used to move a particular gene of interest from a parent vector to a destination vector in order to further study its functionality. Easy Subcloning . by Michael Koelle. Subcloning should be easy and fast, and work every time. ... The ...

Sticky end

...s of the end of a molecule of DNA or a recombinant DNA molecule. The concept is important in molecular biology, especially in cloning or when subcloning insert DNA into vector DNA. All the terms can also be used in reference... ... able to base pair with any DNA molecule containing the comp...

Expression Vector

...rotocol: pCI and pSI Mammalian Expression Vectors , TB206. GenBank® Acc.#: U47119 ... BRIEF ARTICLE DESCRIPTION ... Focus: Expression Vector subcloning ... The Flexi® Vectors are available for expression of N- and C-terminal HaloTag® ... ... expression system include bacterial expression sy...
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