Gene silencer and quantum dots reduce protein production to a whisper

More than 15 years ago scientists discovered a way to stop a particular gene in its tracks. The Nobel Prize-winning finding holds tantalizing promise for medical science, but so far it has been difficult to apply the technique, known as RNA interference, in living cells. Now scientists at the U...

Turning back the Clock in Inherited Anemias

...s also a professor of pediatrics at Harvard Medical School and chair of pediatric oncology at Dana-Farber. "It's pretty clear that this gene is a silencer of fetal hemoglobin. If you could knock it down to a low level, you could turn on fetal hemoglobin." "The discovery of a single gene tha...

Study reveals how stem cells decide to become either skeletal or smooth muscle

...on. The current findings suggest, however, that myocardin has a second role, independent of its partnership with CARG-SRF, where it serves as a potent silencer of gene expression for the stem cell to skeletal muscle gene program. With its dual action, myocardin is an early example of the efficiency and e...

Discovery of widespread tumor growth gene holds promise for effective anti-cancer treatment

...s the presence of two sequence elements in the Trop-2 cytoplasmic tail, the signalling engine of Trop-2, which act as, respectively, an enhancer and a silencer of metastatic propensity. This may be the key to the identification of signalling molecules that promote or inhibit the formation of metastases. I...

Silencer in Biological News

Case Western Reserve finds mechanism underlying alt. splicing of premessenger RNA into messenger RNA

...ream splicing site sometimes silenced it and sometimes did not. Using bioinformatics techniques, the researchers organized the 89 unique intronic silencer sequences they found into four distinct clusters, while the 47 unique exonic silencers formed two clusters. That more than 130 potential mRNA's could ...

Depression model leaves mice with molecular scar

...n mice exposed to this animal model of depression, silencer molecules turned off a gene for a key protein in t...a a process called methylation. Methyl groups, the silencer molecules, attach themselves to the histones, turn...ne. Notably, imipramine was unable to remove these silencer molecules, suggesting that they remained a latent ...

Gene keeps neural cells on correct developmental path

... created the acronym to describe how REST quiets the nerve genes. The protein is also known by the name Anderson gave it, NRSF, for neuron-restrictive silencer factor. Since then, they and others have found that REST locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other m...

Silencer in Biological Technology

Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

Although the Silencer siRNA Labeling Kits were developed for labeling siRNA, they can be readily adapted for labeling long dsRNA. The following protocol can be used for t...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most c...

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs

YOUR DATA: Direct Real-time RT-PCR on Sympathetic Neuron Lysates Silencing of Focal Adhesion Kinases with Silencer Validated siRNAs Direct Real-time RT-PCR on Sympathetic Neuron Lysates Quantitative RT-PCR is the most c...

Biomatrica's RNAstable(TM) Stabilizes RNA at Room Temperature

... room temperature, eliminating the need for cold storage and shipping. Interest in RNA has skyrocketed over the past years due to its role as a gene silencer and in gene expression studies. However, RNA samples are difficult to work with given their highly labile nature and tendency to degrade even in cold ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

...f="http://www.ambion.com/catalog/CatNum.php?1632"> silencer siRNA Labeling Kit (Ambion, Inc.)...f="http://www.ambion.com/catalog/CatNum.php?1632"> silencer siRNA Labeling Kit and evaluated for ...f="http://www.ambion.com/catalog/CatNum.php?1632"> silencer siRNA Labeling Kit (Ambion, Inc.) wit...

Perform RNAi Library Screens on Any Budget

... silencer CellReady siRNA Library System NEW silencer CellReady siRNA Libraries ... Transfection Agent NEW silencer CellReady siRNA Transfection ...

IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

... Genomes silencer Validated siRNAs silencer siRNA Libraries ...A">www.ambion.com/siRNA . silencer Validated siRNAs Function...

GENE SILENCING WITH PCR PRODUCTS

...time intensive. Ambion's new silencer Express siRNA Expression Cas...on and long term studies. silencer Express Kits Ambion's silencer Express Kits provide the reagents nece...

Are siRNA Pools Smart?

... Figure 1. Efficacy of silencer Pre-designed siRNAs. More than 1100 s... Pools. Cells transfected with three individual silencer Pre-designed siRNAs (30 nM) or a pool ...NA expressed in cells transfected with silencer Negative Control #1 siRNA (Ambion). Al...

A New Way to Assess siRNA Delivery Efficiency

...-induced toxicity Use with silencer GAPDH siRNA Controls and silencer CellReady Transfection Optimization Kit...with the transfection process. Ambions silencer GAPDH siRNA is an ideal positive con...

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Silencer in Biological Dictionary

Suppressor

...as a silencer, is a device fixed on ... Suppressor · Flash Hider · Aperture Sight · Telescopic Sight · Rifle Grenade ... Compare Cheap Prices for silencer Suppressor at NexTag.com. Find Bargains on Appliances, Furnishings and More for your Kitchen, Bedroom and Yard. AWC has been delivering world cl...

SiRNA

...A ( siRNA ) for the induction of RNA interference (RNAi) in a variety of organisms. siRNA Synthesis. Premium quality siRNAs ... Made-to-order silencer Select siRNA Libraries ... siRNA /Target Protein Isolation, Detection, & Quantitation ... Predesigned siRNA and siRNA libraries designed...

Ribosomal DNA genes

...nes , all of which are essential for normal ... gene often make the 12S RNA in human cells look similar to bacterial ribosomal RNA. ... ... silencer gene , SIR2, in regulation of recombination in ribosomal DNA . ... An unusual form of transcriptional silencing in yeast ribosomal DNA . Gen...