Innovative yeast genome may handle Cancer Clues
...ancers: this small organism could prove vital in helping to better understand and treat cancer and other disease," said Nurse.
Fission yeast, or schizosaccharomyces
pombe, is the sixth and smallest genome of organisms, including humans, whose cells contain a nucleus to be decoded. Humans have an estimated 30,000-4...
Schizosaccharomyces in Biological News
Scientists develop method for comprehensive proteome analysis
...-- Investigators at Burnham Institute for Medical Research (Burnham) have deciphered a large percentage of the total protein complement (proteome) in schizosaccharomyces
pombe ( S. pombe ) fission yeast.
Laurence Brill, Khatereh Motamedchaboki, Ph.D. and lead investigator Dieter Wolf, Ph.D., developed the novel met...
The structure of the Mre11 protein bound to DNA
...was halted. The 3' strand is held apart and prepared for continuing replication after repair.
Now combining sequencing and biochemical studies of schizosaccharomyces
pombe (fission yeast), by Paul Russell and his lab at Scripps, with models based on images of the P. furiosus Mre11-DNA complexes, the researchers ...
Baumann lab identifies elusive telomere RNA subunit in single cell model
...in these species compared to human cells. Recently, the Baumann Lab used a biochemical approach to identify and clone the RNA subunit of telomerase in schizosaccharomyces
pombe, or fission yeast.
The identification of the fission yeast equivalent of the telomerase RNA gene provides us with a critical tool to study t...
NHGRI targets 12 more organisms for genome sequencing
...ies (Nasonia giraulti and Nasonia longicornis); a free-living soil amoeba (Acanthamoeba castellanii); and three fungi (Schizosaccharomyces octosporus, schizosaccharomyces
japonicus, Batrachochytridium dendrobatidis).
It has been shown that most sequences of the human genome originated long before humans themselves. C...
Schizosaccharomyces in Biological Technology
Fermentation Cell Culture: University Style
Stevens: We primarily grow Saccharomyces
cerevisiae in our laboratory, but we also grow
pombe and Escherichia coli.
BioPharm: For what purpose(s) do you
grow the cultures?
Stevens: For our...
Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
... seconds to 2 minutes over
33 hours, then from 2 minutes to 50 minutes over 55 hours. Under these
conditions, the chromosomes of schizosaccharomyces
pombe (closed circles,
lanes 4 and 6), Saccharomyces cerevisiae (open squares, lanes 3 and 5),
lambda phage concatomers (open ci...
Production of Full-Length, Biologically Active MEK1WT and MEK1CA
cells, such as insect cells or yeast. In this article, we describe the
expression of MEK1 WT and MEK1 CA as GST fusion proteins
pombe using the ESP expression and
purification system. 9 We show that full-length GST-MEK1 WT
and GST-MEK1 CA can be produced in yeast and p...
New Yeast Cloning System for Producing Proteins with Native Amino Acid
...ystem offers rapid
and high-yield production of recombinant proteins in a eukaryotic organism.
Because the system uses the yeast schizosaccharomyces
the expression host, proteins undergo many of the posttranslational modifications
that are often required for their bio...
Simple, Sensitive, and Rapid Detection of FLAG -Tagged
To determine the kits sensitivity, we used a GST-FLAG-calmodulin
fusion protein purified from schizosaccharomyces
pombe as the test
sample. As shown in Figure
3 , as low as 10 ng/ml of the fusion protein can be detected. We further
A New C-Terminal GST Vector for Protein Production in S. pombe
... and tested the new pESP-3 vector, which can be
used for C-terminal fusion with glutathione-S-transferase (GST)
and for protein production in schizosaccharomyces
After expression and purification of the GST fusion protein using the ESP
yeast protein expression and purification system, the GST ta...
Schizosaccharomyces in Biological Products
Schizosaccharomyces in Biological Definition
...rtant for the assembly of the kinetochore on the centromere and may play a role in the epigenetic inheritance of the centromere site.
In the yeast schizosaccharomyces
pombe (and likely in other eukaryotes) the formation of centromeric heterochromatin is connected to RNAi * . In nematodes such as Caenorhabditis ...
Today, the most comprehensively studied and understood fungi are the yeasts and eukaryotic model organisms Saccharomyces cerevisiae and schizosaccharomyces
Many fungi produce toxins , antibiotics , and other secondary metabolites . For example, the cosmopolitian (worldwide) genus Fusarium...
Schizosaccharomyces in Biological Dictionary
...ng to, and inactivating, cyclin-CDK complexes. ... G1 is a crucial phase of cell growth because the decision to begin another ... response of schizosaccharomyces
pombe cells to UV irradiation in early G1 phase . ... a decision in the G1 phase of the division cycle. either to proceed through the c...