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SEQUENASE in Biological Technology

A simple method to sequence from bacterial colonies using [a-33P] radiolabeled ddNTPs and Thermo Sequenase

Introduction In traditional DNA sequencing systems, sequencing products are radioactively labeled using either endlabeled primers or by the incorporation of radiolabeled dNTPs during primer extension. Successful sequencing results produced by these two labeling methods are hi...

Cloning Based on Efficient Three-Fragment Assembly DNA Ligation

...nalysis with the endonuclease Sca I, followed by sequencing of all restriction-positive clones using the primer 5'-TGAATTTTCTGTATGAGG-3', the T7 sequenase 2.0 kit, and 33PdATP from Amersham, according to the manufacturers instructions. Results and Discussion ...
SEQUENASE in Biological Products

Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit from USB Corp.

Description: The Thermo Sequenase Dye Primer Manual Cycle Sequencing Kit is designed to be used with fluorescent dye-labeled primers and high-resolution fluorescence scanners. Sequencing reactions involve a termination step followed by standard slab gel electrophoresis and scanning of the gel to detect fluoresc...
Company:USB Corp.
SEQUENASE in other areas

Sequenase™ Version 2.0 DNA Sequencing Kit - USB Corporation

USB Corporation is an established supplier of reliable biochemicals and molecular biology products fueling innovation in both basic research and emerging technology ...

22 possible paths to "Sequenase" - Biocompare Buyer's Guide For Life ...

... Nucleic Acid Labeling " Labeling Enzymes " DNA Polymerases " Sequenase ... DNA Sequencing " Manual DNA Sequencing " Sequencing Reagents " Sequenase ...

Sequenase PCR Product Sequencing Kit from Affymetrix / USB - Biocompare ...

The Sequenase PCR Product Sequencing Kit utilizes a novel enzymatic clean-up ... primers, ready to be sequenced with standard Sequenase Version 2.0 Kit reagents. ...

The SequiTherm EXCEL™ II DNA Sequencing Kit: A New Kit for Both Cycle ...

EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, ... With the T7 Sequenase kit, Mn2+ can be added to the labeling reaction, or the ...

Sequencing "Difficult DNA" Templates, EPICENTRE® Biotechnologies

EPICENTRE develops and manufactures reagents and kits for genomics, ... Isothermal DNA sequencing is commonly performed at 37°C using Sequenase DNA polymerase. ...

ABC Random Amplification

... which exceeds the capacity of Sequenase (in Part A) would carry ... 0.3 µL Sequenase (13U/µl) 5.05 µl Total Volume. 5. Add 3 µL of Reaction 2 to each tube. ...
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