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SEPHAROSE in Biological Technology

Addition of imidazole during binding improves purity of histidine-tagged proteins

...reduce the binding of contaminating proteins to Ni sepharose media. On one hand, the amount of Ni sepharose can be adapted to the expected amount of protein t...hance the purity of (His) 6 -PknG purified with Ni sepharose High Performance. Determina...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

...urified on His GraviTrap columns prepacked with Ni sepharose 6 Fast Flow without the need for a pump or purific...attached to chelating separation media, such as Ni sepharose 6 Fast Flow. His GraviTrap is a single-use column prepacked with Ni sepharose 6 Fast Flow. His GraviTrap provides high protein b...

The Bio-Rad Silver Stain, Rev E

...try, Clin Chem 28, 10211025 (1982) 24. Gospodarowicz D et al., Isolation of brain fibroblast growth factor by heparin- sepharose affinity chromatography: identity with pituitary fibroblast growth factor, Proc Natl Acad Sci USA 81, 69636967 (1984) 25. Grove B...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

...ification of GST-tagged proteins using glutathione sepharose usually results in lower degree of non-specific co...r plus column was loaded with 150 l of glutathione sepharose resin and washed three times with 500 l of PBS bef...nd the GST-tagged protein bound to the glutathione sepharose was eluted with 350 l of 20 mM Tris-HCl plus 10 mM...

TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

...lls (following manufacturers instructions), and the fusion proteins from 3 ml of bacterial cultures were purified on glutathione sepharose as described. 7 While Coomassie Blue staining revealed roughly equivalent protein levels from each strain, immunoblot analyse...
SEPHAROSE in Biological Products

Calmodulin Sepharose 4B from GE Healthcare, formerly Amersham Biosciences

Description: Calmodulin Sepharose 4B, 10 ml. For single-step purification of calmodulin-binding peptide fusion proteins.Purification of calmodulin-regulated proteins from all eukaryotic cells. Category: Chromatography Columns & Media, Affinity, Group Specific Affinity....
Company:GE Healthcare, formerly Amersham Biosciences

Streptavidin Sepharose High Performance from GE Healthcare, formerly Amersham Biosciences

Description: Streptavidin Sepharose High Performance, 5 ml. The same high performance streptavidin media used in HiTrap Streptavidin columns for fast, reliable purification of biotinylated compounds.Ideal for packing into Tricorn 5/50 GL, XK 16/20, or other columns when larger scale purifications are required.E...
Company:GE Healthcare, formerly Amersham Biosciences

REC. PROTEIN G - SEPHAROSE from Invitrogen

Description: Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants...
Company:Invitrogen

REC. PROTEIN G - SEPHAROSE from Invitrogen

Description: Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants...
Company:Invitrogen

REC PROTEIN A - SEPHAROSE from Invitrogen

Description: Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants...
Company:Invitrogen

SizeSep 400 Spun Columns from GE Healthcare, formerly Amersham Biosciences

Description:...Sep 400 Spun Columns, 10. *SizeSep 400 Spun Columns: For rapid intermediate purification of cDNAs > 400 base pairs in length. - *Prepacked with sepharose CL-4B and pre-equilibrated in distilled water containing 0.15% Kathon CG/IcP Biocide. *Remove all cDNA smaller than ~ 400 base pairs. *Extremely us...
Company:GE Healthcare, formerly Amersham Biosciences

ReBlot Plus Kit from CHEMICON

Description:... While antigen and antibody-based immunoaffinity matrices, such as sepharose conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded...
Company:CHEMICON
SEPHAROSE in Biological Definition

Affinity chromatography

...ST has an affinity for glutathione (commercially available as glutathione sepharose ). For elution purposes glutathione is used. Another use for the procedur...well. The protein can then be covalently coupled to a solid medium such as sepharose or agarose (these are commercially available). For thoroughness the GST pro...

Molecular biology

...st down with it) whereas all other proteins will remain in the solution. Alternatively, antibodies coupled to a solid support matrix like Sephadex or sepharose beads, for example, can be used to remove a protein of interest from a complex solution. After washing unbound and non-specifically bound materials a...
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(Date:4/23/2014)... health benefits of probiotics, antioxidants and other nutritional compounds, ... chemical industry is getting in on the action. But ... so the industry is responding with clinical research to ... in Chemical & Engineering News (C&EN), the ... Melody M. Bomgardner, a senior editor at C&EN, writes ...
(Date:4/23/2014)... as the United States, it may come as a surprise ... adequate food due to lack of money or other financial ... the University of Illinois using data from the National Health ... are dealing with hunger are also facing negative health and ... over age 60 faced the threat of hungerthat translates to ...
(Date:4/23/2014)... by a researcher from the Cancer Science Institute of ... has identified the cancer specific stem cell which causes ... developing new drugs for the treatment of this disease ... led by Dr Chan Shing Leng, Research Assistant Professor ... a cancer-specific variant of a cell surface protein, CD44v8-10, ...
(Date:4/23/2014)... Inverse Effects of Midlife Occupational and Leisure Time Physical ... occupational physical activity in midlife increases the risk of ... decreases the risk. This is found in a study ... years. The study was conducted at the Gerontology Research ... Health. , Heavy physical labor is often repetitive, wears ...
(Date:4/23/2014)... also known as Ritalin, may prevent the depletion of ... , a journal of the Association for Psychological Science. ... diet or trying to focus attention on a boring ... one potential explanation for this difficulty: Exerting self-control for ... exert self-control effectively on subsequent tasks. , "It is ...
Breaking Medicine News(10 mins):Health News:More Americans in their golden years are going hungry 2Health News:Scientists identify cancer specific cell for potential treatment of gastric cancer 2Health News:Scientists identify cancer specific cell for potential treatment of gastric cancer 3Health News:Midlife occupational and leisure-time physical activity limits mobility in old age 2Health News:ADHD drug may help preserve our self-control resources 2
(Date:4/23/2014)... HUNTSVILLE, TX (4/23/14) -- Researchers have found evidence ... risk factors that increase aggressive behavior in children, ... that not all children exposed to prenatal smoking ... others will not," said Brian Boutwell, Assistant Professor ... Justice and senior author on the study. ...
(Date:4/23/2014)... as an antimicrobial ingredient in soaps, toothpastes and other ... new evidence that appears to support these worries. Their ... in Toxicology , found that triclosan, as well as ... human breast cancer cells in lab dishes and breast ... note that hormonal imbalances seem to play a role ...
(Date:4/23/2014)... to date of the family of bacteria that ... strategies and reveals surprising findings about the bacteria,s ... public health strategies to control this respiratory disease, ... Genomic analysis of 343 strains of the ... collected over the last 100 years illustrates how ...
Breaking Biology News(10 mins):Genetics risk, prenatal smoking may predict behavioral problems 2In lab tests, the antimicrobial ingredient triclosan spurs growth of breast cancer cells 2Impact of whooping cough vaccination revealed 2
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