Addition of imidazole during binding improves purity of histidine-tagged proteins

...reduce the binding of contaminating proteins to Ni sepharose media. On one hand, the amount of Ni sepharose can be adapted to the expected amount of protein t...hance the purity of (His) 6 -PknG purified with Ni sepharose High Performance. Determina...

New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

...urified on His GraviTrap columns prepacked with Ni sepharose 6 Fast Flow without the need for a pump or purific...attached to chelating separation media, such as Ni sepharose 6 Fast Flow. His GraviTrap is a single-use column prepacked with Ni sepharose 6 Fast Flow. His GraviTrap provides high protein b...

The Bio-Rad Silver Stain, Rev E

...try, Clin Chem 28, 10211025 (1982) 24. Gospodarowicz D et al., Isolation of brain fibroblast growth factor by heparin- sepharose affinity chromatography: identity with pituitary fibroblast growth factor, Proc Natl Acad Sci USA 81, 69636967 (1984) 25. Grove B...

Double-Affinity Purification of GST-His Tagged Proteins Using Vivapure 8-to-96 Well Cobalt-Chelate Kit

...ification of GST-tagged proteins using glutathione sepharose usually results in lower degree of non-specific co...r plus column was loaded with 150 l of glutathione sepharose resin and washed three times with 500 l of PBS bef...nd the GST-tagged protein bound to the glutathione sepharose was eluted with 350 l of 20 mM Tris-HCl plus 10 mM...

TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins

...lls (following manufacturers instructions), and the fusion proteins from 3 ml of bacterial cultures were purified on glutathione sepharose as described. 7 While Coomassie Blue staining revealed roughly equivalent protein levels from each strain, immunoblot analyse...

SEPHAROSE in Biological Products

Calmodulin Sepharose 4B from GE Healthcare, formerly Amersham Biosciences

Description: Calmodulin Sepharose 4B, 10 ml. For single-step purification of calmodulin-binding peptide fusion proteins.Purification of calmodulin-regulated proteins from all eukaryotic cells. Category: Chromatography Columns & Media, Affinity, Group Specific Affinity....
Company:GE Healthcare, formerly Amersham Biosciences

Streptavidin Sepharose High Performance from GE Healthcare, formerly Amersham Biosciences

Description: Streptavidin Sepharose High Performance, 5 ml. The same high performance streptavidin media used in HiTrap Streptavidin columns for fast, reliable purification of biotinylated compounds.Ideal for packing into Tricorn 5/50 GL, XK 16/20, or other columns when larger scale purifications are required.E...
Company:GE Healthcare, formerly Amersham Biosciences

REC. PROTEIN G - SEPHAROSE from Invitrogen

Description: Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants...
Company:Invitrogen

REC. PROTEIN G - SEPHAROSE from Invitrogen

Description: Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants...
Company:Invitrogen

REC PROTEIN A - SEPHAROSE from Invitrogen

Description: Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants...
Company:Invitrogen

SizeSep 400 Spun Columns from GE Healthcare, formerly Amersham Biosciences

Description:...Sep 400 Spun Columns, 10. *SizeSep 400 Spun Columns: For rapid intermediate purification of cDNAs > 400 base pairs in length. - *Prepacked with sepharose CL-4B and pre-equilibrated in distilled water containing 0.15% Kathon CG/IcP Biocide. *Remove all cDNA smaller than ~ 400 base pairs. *Extremely us...
Company:GE Healthcare, formerly Amersham Biosciences

ReBlot Plus Kit from CHEMICON

Description:... While antigen and antibody-based immunoaffinity matrices, such as sepharose conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded...
Company:CHEMICON

SEPHAROSE in Biological Definition

Affinity chromatography

...ST has an affinity for glutathione (commercially available as glutathione sepharose ). For elution purposes glutathione is used. Another use for the procedur...well. The protein can then be covalently coupled to a solid medium such as sepharose or agarose (these are commercially available). For thoroughness the GST pro...

Molecular biology

...st down with it) whereas all other proteins will remain in the solution. Alternatively, antibodies coupled to a solid support matrix like Sephadex or sepharose beads, for example, can be used to remove a protein of interest from a complex solution. After washing unbound and non-specifically bound materials a...