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RiboGreen in Biological Technology

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...04) ◊ RNAse-Free DNase Set (Qiagen, Cat. #79254) ◊ ribogreen RNA quantification kit (Molecular Probes, Cat. #R11490) V. REAL-...
  • RNA concentration was measured by a ribogreen sensitive nucleicacid dye binding assay according to manufacturers instruct...

    Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

    ...ies). Total RNA was DNasetreated using DNA-free reagent (Ambion), and RNA yields were determined by fluorescence at 530 nm using ribogreen RNA quantitation reagent (Molecular Probes) as described by the manufacturer. First-strand cDNAs were synthesized from equal amo...

    Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer

    ...tandard and all samples were diluted in TE buffer and mixed with ribogreen (Molecular Probes, Inc.) that had been diluted 2,000-fold. Fluor...3833 (1996). 2. Molecular Probes, Inc. Product Information Sheet, ribogreen RNA quantitation reagent (1997). 3. Ausubel, F., et al...

    RNAPrep - Total RNA Isolation Purification

    ...otal RNA from 1x10 5 293T cells was isolated with RNAPrep and two other competitor methods, Q & P. Quantitation was performed by ribogreen assay. RNAPrep yielded 1.5-3.0 times greater yield than competitors Q & P. Figure 1B. Total RNA isolated from 100 to 1x10 6 36...

    Rapidly Synthesize Large Amounts of Capped RNA

    ... indicated incubation times, each reaction was DNase treated as per protocol and a preliminary quantitatation was performed with ribogreen (Molecular Probes). Each reaction was then purified using the
    (Date:4/24/2014)... Scientific research at UT Southwestern Medical Center previously ... completely, whereas the adult heart lacks this ability. New ... the heart loses its incredible regenerative capability in adulthood, ... , Yes, oxygen. It is well-known that a major ... throughout the body. But at the same time, oxygen ...
    (Date:4/24/2014)... Changes in managing patients before, during and after colorectal ... readmission rates, according to researchers who led a study ... practice, called enhanced recovery, is easier on patients before ... evacuation that are typically prescribed. After surgery, patients are ... possible, leading to faster recoveries. , Among findings published ...
    (Date:4/24/2014)... terminology, and the relevance of palliation in advanced ... randomized controlled trials (RCTs). This is the result ... Quality and Efficiency in Health Care (IQWiG), which ... Together with external experts, IQWiG analysed studies on ... malignant melanoma, and pancreatic cancer. For this purpose, ...
    (Date:4/24/2014)... A Finnish team of researchers was the first in ... which is a tumour of the jaw. , Ameloblastoma ... recur after treatment. Ameloblastoma is most often found in ... by surgery, often resulting in tissue deficiencies in the ... suitable drug therapy could reduce the need for surgery ...
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  • (Date:4/24/2014)... turtle is the largest river turtle in North America, ... almost a century. Now researchers from Florida and the ... one species but three. , Examining museum specimens ... in this ancient reptile. , Once heavily hunted for ... of Campbell,s Turtle Soup in the 1960s the ...
    (Date:4/24/2014)... RICHLAND, Wash. A common plant puts out a welcome ... the mat,s molecular mix. , The study published this week ... reveals new targets during the battle between microbe and ... team showed that the humble and oft-studied plant Arabidopsis ... from a pathogen. It,s as if a hostile army were ...
    (Date:4/23/2014)... "playwright" to their resumes in an effort to aid ... ethical issues as genomic science transitions to bedside healthcare, ... , Until now the plays of Karen H. ... and by, their peers and others in the genomics ... With Oxford University Press, publication of the book ...
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