Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions
◊ RNAse-Free DNase Set (Qiagen, Cat. #79254)
RNA quantification kit (Molecular Probes, Cat. #R11490)
RNA concentration was measured by a ribogreen sensitive nucleicacid dye binding assay according to manufacturers instruct...
Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A...ies). Total RNA was DNasetreated
using DNA-free reagent (Ambion), and RNA yields were determined by fluorescence
at 530 nm using ribogreen RNA quantitation reagent (Molecular Probes)
as described by the manufacturer. First-strand cDNAs were synthesized
from equal amo...
Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer...tandard and all samples were diluted
in TE buffer and mixed with ribogreen (Molecular Probes, Inc.) that had
been diluted 2,000-fold. Fluor...3833 (1996).
2. Molecular Probes, Inc. Product Information Sheet, ribogreen RNA quantitation
3. Ausubel, F., et al...
RNAPrep - Total RNA Isolation Purification...otal RNA from 1x10 5 293T cells was isolated with RNAPrep
and two other competitor methods, Q & P. Quantitation was performed
by ribogreen assay. RNAPrep yielded 1.5-3.0 times greater yield than competitors
Q & P.
Figure 1B. Total RNA isolated from 100 to 1x10 6 36...
Rapidly Synthesize Large Amounts of Capped RNA... indicated incubation
times, each reaction was DNase treated as per protocol and a preliminary
quantitatation was performed with ribogreen (Molecular Probes). Each
reaction was then purified using the
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