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Recombinase in Medical News

Potential Cure for HIV Discovered

...ells, he wrote. "Although favorable results would represent perhaps only a baby step toward eventual use in patients, the discovery of the Tre recombinase proves that enzymatic removal of integrated HIV-1 from human chromosomes is a current-day reality," he said. The researchers who developed the...
Recombinase in Biological Technology

Mammalian Expression Vectors with Interchangeable Selectable Markers

... pExchange core expression vector, drug-resistance modules and Cre recombinase protein. The pExchange core vectors are based on the pCMV-Script a...recombinase. This reaction is followed by heat inactivation of Cre recombinase at 65C for 15 to 30 minutes. The inactivated recombination reactio...
Recombinase in Biological Products

* Quick & Easy E. coli Gene Deletion Kit from Gene Bridges GmbH

Description:... The use of an FRT-flanked resistance cassette for the replacement of the targeted gene allows the subsequent removal of the selection marker by a Flp recombinase step, if required. Flp expression plasmids can be purchased from Gene Bridges. Multiple knockouts can be generated either by a repetitive insertion o...
Company:Gene Bridges GmbH

* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH

Description:...eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cells. The included pCI-FLPe expression plasmid codes for an improved Flp recombinase which shows a four to tenfold higher recombinational activity compared to the wildtype Flp enzyme at temperatures between 37C (optimal growth temperat...
Company:Gene Bridges GmbH
Recombinase in Biological Definition

Genetic recombination

...set of genetic information. Chemistry of crossover Enzymes known as recombinases catalyze the reactions that allow for crossover to occur. A recombinase creates a nick in one strand of a DNA double helix, allowing the nicked strand to pull apart from its complementary strand and anneal to one stran...
Recombinase in Biological Dictionary

Recombinase

Cre recombinase , often abbreviated to Cre, is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. The enzyme does not require any energy cofactors and Cre-mediated recombination quickly reaches equilibrium between subst...
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