DNA Recombination and Repair—A New Twist to RecA Function

Molecular motors harness the energy of ATP (or GTP, a related energy currency) and transform it into mechanical force. Well-known examples of motors include myosin and dynein, proteins that use ATP to ferry intracellular cargo along fibers made of actin or tubulin proteins. The ATP-dependent assemb...

Recombination protein dynamics observed with single monomer resolution

...report in the August issue of the journal Cell. reca is a DNA recombination protein found in the gut ba...n filament." During the recombination process, reca binds with DNA to form a filament that spirals aro... The technique revealed intricate details of how reca nucleates to form a filament, how the filament cha...

RecA in Biological Products

NovaBlue Glycerol Stock from Novagen

Description:...n ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and reca endA mutations, which result in high yields of excellent quality plasmid DNA. The DE3 lysogen of NovaBlue is potentially useful as a stringent host du...
Company:Novagen

RecA in Biological Dictionary

Sub-cloning

...ng step in the entire sub - cloning process. ... DOI: 10.1038/nprot.2007.121. SLIC Sub - cloning using T4 DNA polymerase treated inserts with reca ... We describe a novel cloning method SLIC that allows the ... BIOSCI/Bionet News .. Biosequences .. Software .. FTP. sub - cloning and prote...

Shuttle vector

...e simplify the shuttle vector cloning to one step by incorporation ... We hypothesized that a shuttle vector containing the R6kγ origin, the reca gene ... The shuttle vectors do not integrate into the genome and do not rearrange. ... We used the plasmid pRN1 as starting point to con...

RecA protein

... reca is a 38 kilodalton Escherichia coli protein essential for the repair and maintenance of DNA. reca has a structural and functional homolog in every ...e for this class of homologous DNA repair... reca promotes the autodigestion of the lexA repressor,...

Heteroduplex Dna

... that heteroduplex DNA formation requires three distinct enzymatic ... The rate-limiting step of heteroduplex DNA formation is the trapping by reca ... Psychology research, information, analysis and reviews from Psychology Journals, Newspapers, and magazines at HighBeam.com. Free Trial, Credit ...

Direct Repeats

...ments are located in the same orientation in a ... Direct complementary repeats - two segments are located in the same orientation ... ... coli reca strains with a plasmid that contains direct repeats of these sequences. ... structures containing direct repeats of IS50 or Tn5 during the ....

Branch migration

...hexamer ... found that the crosslink failed to inhibit branch migration . ... RuvAB was found to promote branch migration , to dissociate the reca filament, ... of RuvAB and ATP, rapid branch migration through the nucleosome is observed. ... Two models for the mechanism of branch migr...

Antigenic determinant

...cts with human rat dog), anti-KIN, KIN (KIN. antigenic determinant of reca protein homolog (mouse)) antibody, rabbit ... Immunochemistry: Uncerta... from 70 cDNA libraries. ... Homo sapiens KIN, antigenic determinant of reca protein homolog (mouse), mRNA ... Multiple Sclerosis Encyclopaedia - ...