Navigation Links
RPMI in Medical Technology

Gentium's Defibrotide Highlighted in Two Poster Presentations at American Association for Cancer Research Annual Meeting

... This in vitro study investigated Defibrotide's effectiveness in regulating the expression and activity of heparanase in two MM cell lines, U266 and rpmi 8226. The potential effect of Defibrotide to regulate heparanase gene expression was evaluated through real time polymerase chain reaction (RT-PCR) of...
RPMI in Biological Technology

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

...ted here. Methods Jurkat cells (clone E6-1 from ATCC), a leukemic human T-cell line, were maintained in rpmi 1640 media with 2 mM L-glutamine and supplemented with 2.5 g/l glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, in addition to ...

Transformation of Nematodes With the Helios Gene Gun

... host Mastomys coucha (Wegerhof and Zahner, 1985), and stored in rpmi medium (supplemented with 10% FCS, Gibco-BRL; 1% Penicillin and ... female parasites may be cultivated at 37 C for up to 2 weeks in rpmi medium (5% CO 2 ). SELECTION FOR TRANSFORMED TRAITS O...

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

... Syrian hamsters, Mesocricetus auratus. Adult worms were obtained by perfusion at day 42 post-infection, and washed 3 times with rpmi 1640 containing 10% fetal calf serum, before they were kept in 60 mm petri dishes at 37C, 5% CO 2 (Grevelding et al. 1997). Isolation of ...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

... the flow cytometer data (figure 6). Materials and methods Cells were cultured in rpmi medium containing 10 % fetal calf serum, penicillin/streptomycine, 1 mM sodium pyruvate and 2 mM L-glutamine a) Extracellu...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

... the flow cytometer system. Materials and methods Cells were cultured in rpmi medium containing 10 % fetal calf serum, penicillin/streptomycine, 1 mM Sodium Pyruvate and 2 mM Lglutamine. a) Induction of...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

...case, the serum-free medium from PromoCell was clearly advantageous for proliferation, showing an increase of almost 50 % in comparison with the rpmi standard medium, irrespective of whether the colorimetric assay (+50 %) or the chemiluminescence assay (+49 %) was used. ...

Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments

...d Methods Cell culture Cells of the human adherent cell line PC3 were cultured in rpmi 1640 medium containing 10% (v/v) fetal calf serum and 2mM L-glutamine. Cells of the human adherent cell line HCT116 were cultured in McCoys 5A med...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

... Cell culture Cells of the human adherent cell line PC3 stably transfected with the gene for the GFP (PC3-GFP) were cultured in rpmi 1640 containing 10% (v/v) fetal calf serum, 2 mM L-glutamine and 750 g/ml neomycin. Two days prior to transfection with siRNA oligonucleotides, ...

Transfection Reagent Selector Kit Handbook

... glutamine and can include nonessential amino acids, sodium pyruvate, and antibiotics. Some common media include DMEM, F12, DMEM/F12, rpmi 1640, MEM, and S-MEM. Serum In most cases media are supplemented shortly before use with serum. Fet...

The QIAGEN Guide to Animal Cell Culture

... determined experimentally. Common basal media include Eagle minimal essential medium (MEM), Dulbeccos modified Eagle medium (DMEM), rpmi 1640, and Ham F10. These contain a mixture of amino acids, glucose, salts, vitamins, and other nutrients, and are available either a...
RPMI in Biological Products

GIBCO RPMI Medium 1640 (1X) liquid from Invitrogen

Description: ...
Company:Invitrogen

GIBCO RPMI Medium 1640; powder from Invitrogen

Description: ...
Company:Invitrogen

GIBCO RPMI Medium 1640; powder from Invitrogen

Description: ...
Company:Invitrogen

Raji cell line slides ( human: B lymphocyte; Burkitt's lymphoma ) from GeneTex

Description:... Human Raji cells were cultured in rpmi 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in ...
Company:GeneTex

MOLT - 4 cell line slides ( human: T lymphoblast; acute lymphoblastic leukemia ) from GeneTex

Description:... Human MOLT-4 cells were cultured in rpmi 1640 with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in acetone...
Company:GeneTex

A - 20 cell line slides ( mouse: B lymphocyte; reticulum cell sarcoma ) from GeneTex

Description:... Mouse A-20 cells were cultured in rpmi 1640 with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in acetone...
Company:GeneTex
Other Tags
(Date:11/26/2014)... The Maryland Economic Development Association (MEDA) ... MEDA Awards, which, sponsored by Miles and Stockbridge ... MEDA Awards recognize the individuals, programs and projects ... encourage our members to nominate the individuals, programs ... impact in the state of Maryland,” said Pam ...
(Date:11/26/2014)... Francisco, California (PRWEB) November 26, 2014 ... (ADR) and stringent drug safety regulations are expected ... the forecast period. Growing prevalence of acute and ... in demand for new drug development. Moreover, growing ... reporting about the same has become the need ...
(Date:11/26/2014)... Pass, OR (PRWEB) November 26, 2014 ... related fresh water shortages, is moving forward with a ... into drinking water.* Radio host Sharon Kleyne supports the ... of “Total Water Management” (TWM). According to Kleyne, by ... to constantly import new water could be greatly reduced. ...
(Date:11/26/2014)... WOODLAND HILLS, CA (PRWEB) November 26, 2014 ... implants costing thousands of dollars, the prosthodontists at the Anacapa ... that paying for dental procedures can be daunting for some. ... to pay for the procedures they offer. , “No dentist ... functioning and beautiful set of teeth,” said Dr. Mamaly Reshad, ...
(Date:11/26/2014)... Schaumburg, IL (PRWEB) November 26, 2014 ... of communication management solutions, has been award the ... Telephony Magazine. , This award comes as a ... its capabilities within the Lync ecosystem. Specifically, ISI ... for all modes of Lync communication, including voice, ...
Breaking Medicine News(10 mins):Health News:Maryland Economic Development Association Seeking Submissions for 2015 Awards 2Health News:Pharmacovigilance Market To 2020 - Global Industry Growth, Size, Share, Trends, Analysis And Forecast To 2020 Research Report By Grand View Research, Inc. 2Health News:Pharmacovigilance Market To 2020 - Global Industry Growth, Size, Share, Trends, Analysis And Forecast To 2020 Research Report By Grand View Research, Inc. 3Health News:Global fresh water advocate supports San Diego wastewater recycling plan 2Health News:Global fresh water advocate supports San Diego wastewater recycling plan 3Health News:Anacapa Dental Art Institute Doctors Offers 6 Tips on How to Pay for Cosmetic, Restorative and Implant Dentistry 2Health News:Anacapa Dental Art Institute Doctors Offers 6 Tips on How to Pay for Cosmetic, Restorative and Implant Dentistry 3Health News:Anacapa Dental Art Institute Doctors Offers 6 Tips on How to Pay for Cosmetic, Restorative and Implant Dentistry 4Health News:Anacapa Dental Art Institute Doctors Offers 6 Tips on How to Pay for Cosmetic, Restorative and Implant Dentistry 5Health News:ISI Telemanagement Solutions, Inc. has been Awarded the 2014 Microsoft Lync Pioneer Award 2
(Date:11/7/2014)... professor, biomedical engineering, in the Grove School of ... have identified a molecule that could lead to ... forms of breast cancer. , Triple negative breast ... to aggressive proliferation and metastasis and a lack ... discovered the overexpression of intercellular adhesion molecule-1 (ICAM-1) ...
(Date:11/7/2014)... -- Leading Biometric companies aim to provide consumers ... transactions.  Companies in focus today are: NXT-ID, Inc. (NASDAQ: ... ), Google Inc. (NASDAQ: GOOG ), Microsoft Corporation ... EBAY ) and MasterCard Inc. (NYSE: MA) NXT-ID, ... authentication company focused on the growing mobile commerce is pleased ...
(Date:11/6/2014)... Most cancer deaths occur because of metastasis, yet ... has been slow. , "It,s been particularly challenging ... Taran Gujral, research fellow in systems biology at ... aren,t detected until after they,ve already metastasized." , ... culprit that should help researchers better understand how ...
Breaking Biology News(10 mins):Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6Migration negation 2Migration negation 3
Other Contents