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RPMI in Medical Technology

Gentium's Defibrotide Highlighted in Two Poster Presentations at American Association for Cancer Research Annual Meeting

... This in vitro study investigated Defibrotide's effectiveness in regulating the expression and activity of heparanase in two MM cell lines, U266 and rpmi 8226. The potential effect of Defibrotide to regulate heparanase gene expression was evaluated through real time polymerase chain reaction (RT-PCR) of...
RPMI in Biological Technology

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

...ted here. Methods Jurkat cells (clone E6-1 from ATCC), a leukemic human T-cell line, were maintained in rpmi 1640 media with 2 mM L-glutamine and supplemented with 2.5 g/l glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, in addition to ...

Transformation of Nematodes With the Helios Gene Gun

... host Mastomys coucha (Wegerhof and Zahner, 1985), and stored in rpmi medium (supplemented with 10% FCS, Gibco-BRL; 1% Penicillin and ... female parasites may be cultivated at 37 C for up to 2 weeks in rpmi medium (5% CO 2 ). SELECTION FOR TRANSFORMED TRAITS O...

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

... Syrian hamsters, Mesocricetus auratus. Adult worms were obtained by perfusion at day 42 post-infection, and washed 3 times with rpmi 1640 containing 10% fetal calf serum, before they were kept in 60 mm petri dishes at 37C, 5% CO 2 (Grevelding et al. 1997). Isolation of ...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

... the flow cytometer data (figure 6). Materials and methods Cells were cultured in rpmi medium containing 10 % fetal calf serum, penicillin/streptomycine, 1 mM sodium pyruvate and 2 mM L-glutamine a) Extracellu...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

... the flow cytometer system. Materials and methods Cells were cultured in rpmi medium containing 10 % fetal calf serum, penicillin/streptomycine, 1 mM Sodium Pyruvate and 2 mM Lglutamine. a) Induction of...

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

...case, the serum-free medium from PromoCell was clearly advantageous for proliferation, showing an increase of almost 50 % in comparison with the rpmi standard medium, irrespective of whether the colorimetric assay (+50 %) or the chemiluminescence assay (+49 %) was used. ...

Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments

...d Methods Cell culture Cells of the human adherent cell line PC3 were cultured in rpmi 1640 medium containing 10% (v/v) fetal calf serum and 2mM L-glutamine. Cells of the human adherent cell line HCT116 were cultured in McCoys 5A med...

Quantification of siRNA Silencing Efficiency Using the LightCycler System

... Cell culture Cells of the human adherent cell line PC3 stably transfected with the gene for the GFP (PC3-GFP) were cultured in rpmi 1640 containing 10% (v/v) fetal calf serum, 2 mM L-glutamine and 750 g/ml neomycin. Two days prior to transfection with siRNA oligonucleotides, ...

Transfection Reagent Selector Kit Handbook

... glutamine and can include nonessential amino acids, sodium pyruvate, and antibiotics. Some common media include DMEM, F12, DMEM/F12, rpmi 1640, MEM, and S-MEM. Serum In most cases media are supplemented shortly before use with serum. Fet...

The QIAGEN Guide to Animal Cell Culture

... determined experimentally. Common basal media include Eagle minimal essential medium (MEM), Dulbeccos modified Eagle medium (DMEM), rpmi 1640, and Ham F10. These contain a mixture of amino acids, glucose, salts, vitamins, and other nutrients, and are available either a...
RPMI in Biological Products

GIBCO RPMI Medium 1640 (1X) liquid from Invitrogen

Description: ...
Company:Invitrogen

GIBCO RPMI Medium 1640; powder from Invitrogen

Description: ...
Company:Invitrogen

GIBCO RPMI Medium 1640; powder from Invitrogen

Description: ...
Company:Invitrogen

Raji cell line slides ( human: B lymphocyte; Burkitt's lymphoma ) from GeneTex

Description:... Human Raji cells were cultured in rpmi 1640 medium with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in ...
Company:GeneTex

MOLT - 4 cell line slides ( human: T lymphoblast; acute lymphoblastic leukemia ) from GeneTex

Description:... Human MOLT-4 cells were cultured in rpmi 1640 with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in acetone...
Company:GeneTex

A - 20 cell line slides ( mouse: B lymphocyte; reticulum cell sarcoma ) from GeneTex

Description:... Mouse A-20 cells were cultured in rpmi 1640 with 2 mM L-glutamine and harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in acetone...
Company:GeneTex
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(Date:7/24/2014)... July 24, 2014 (HealthDay News) -- Drinking caffeine may ... roughly two-thirds of women as they go through menopause, ... preliminary, our study suggests that limiting caffeine intake may ... hot flashes and night sweats," said researcher Dr. Stephanie ... Mayo Clinic in Rochester, Minn. But caffeine -- ...
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(Date:7/24/2014)... gathered at NJIT this week for an all-day public ... Environmental Protection Agency (EPA) to dredge toxic sediment from ... The EPA has called the plan, which proposes removing ... sections of the riverbed, one of the largest cleanups ... dredged and disposed off-site. , "We need to ensure ...
(Date:7/24/2014)... A yearlong study funded by the New Jersey ... J. Bloustein School of Planning and Public Policy at ... Sandy-affected towns are skeptical about the likelihood of community-based ... respondents, 45 percent indicated they were "pessimistic" or "very ... Superstorm Sandy would be rebuilt better than they were ...
(Date:7/24/2014)... of fires covered central Africa in mid-July 2014, as ... red hotspots, which indicate areas of increased temperatures, are ... Democratic Republic of the Congo (northeast), and Zambia (southeast). ... and in some areas, especially in the Democratic Republic ... the south. , The fire season is an annual ...
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