RNAqueous-96 RNA Isolation Kit Automation Protocol
Throughput RNA Purification with Ambion rnaqueous
Kits on the Robbins
Tango Liquid Handling System (PDF, 136 k)
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... the lysate were stored for two months
at -80C or -20C. Ambion's rnaqueous
Kit was then used to isolate total
RNA from each aliquot along w... isolated from an equivalent number of cells
processed using the rnaqueous
procedure is shown on the far right. The
RNA samples were analyz...
Protect RNA and Improve Target Cell Identification
... either unstained
or stained with three different dyes (Ambion's Cresyl Violet and Acridine
Orange, and a Competitor Stain). The rnaqueous
Micro Kit (Ambion) was
used to process samples that were stained with the LCM Staining Kit. The
Competitor's RNA isolation kit a...
DNA-free A NEW Method to Remove DNA
...nding method [e.g.
RNeasy (Qiagen), rnaqueous
binding method such as rnaqueous
(see the sidebar at right, "RNA
Iso..., using microfuge
tubes as mortars. The rnaqueous
procedure involves disrupting tissues
Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
...ples were stained with DAPI and
analyzed by fluorescence microscopy. Total RNA was isolated from the other
two sample sets using rnaqueous
MAG-96 and then converted to cDNA. Target
cDNA levels were analyzed in duplicate using a TaqMan Gene Expression
Assay for surviv...
Confirming RNAi Effects Is Now Easier Than Ever
...ples were stained
with DAPI and analyzed by fluorescence microscopy. Total RNA was isolated
from the other two sample sets using rnaqueous
MAG-96 and then converted
to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan
Gene Expression Assay for surviv...
Optimum FFPE RNA Isolation Kit
... was fixed in formalin for 24 hours and embedded in paraffin. Two, 10 m
sections were used for RNA isolation. RNA was isolated using the rnaqueous
Micro Kit for the frozen tissue and Optimum FFPE RNA Isolation Kit for
FFPE tissue, respectively.
IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
...ells using siPORT Lipid
at the final siRNA concentrations shown. Forty-eight hours after transfection,
RNA was recovered using rnaqueous
MAG-96 RNA Isolation Kit and reverse
transcribed using the RETROscript Kit. Target cDNA levels were measured
by real-time PCR an...
GENE SILENCING WITH PCR PRODUCTS
... SECs were transfected into 293 cells and RNA from the cells was analyzed
after the indicated number of days. RNA was isolated with the rnaqueous
Kit and then GAPDH mRNA levels were assessed by Northern blot using the
NorthernMax-Gly Kit. The data are expressed as a percent...
Bacterial mRNA Isolation, Fast and Easy
...ess can be used with total
RNA obtained by a variety of methods, including glass fiber filter-based
methods (such as Ambion's rnaqueous
Kits), and phenol-based methods (such
as Ambion's RiboPure-Bacteria Kit). The mRNA isolated with MICROB Express
is a superi...