RNAqueous-96 RNA Isolation Kit Automation Protocol

... href="http://www.ambion.com/techlib/applications/rnaqueous96_robbins.pdf">High Throughput RNA Purification with Ambion rnaqueous Kits on the Robbins Tango Liquid Handling System (PDF, 136 k) back to top ...

Ready for Real-time in 5 Minutes

... the lysate were stored for two months at -80C or -20C. Ambion's rnaqueous Kit was then used to isolate total RNA from each aliquot along w... isolated from an equivalent number of cells processed using the rnaqueous procedure is shown on the far right. The RNA samples were analyz...

Protect RNA and Improve Target Cell Identification

... either unstained or stained with three different dyes (Ambion's Cresyl Violet and Acridine Orange, and a Competitor Stain). The rnaqueous Micro Kit (Ambion) was used to process samples that were stained with the LCM Staining Kit. The Competitor's RNA isolation kit a...

DNA-free A NEW Method to Remove DNA

...nding method [e.g. RNeasy (Qiagen), rnaqueous (Ambion)] 3 ...glass-filter binding method such as rnaqueous (see the sidebar at right, "RNA Iso..., using microfuge tubes as mortars. The rnaqueous procedure involves disrupting tissues o...

Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

...ples were stained with DAPI and analyzed by fluorescence microscopy. Total RNA was isolated from the other two sample sets using rnaqueous MAG-96 and then converted to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan Gene Expression Assay for surviv...

Confirming RNAi Effects Is Now Easier Than Ever

...ples were stained with DAPI and analyzed by fluorescence microscopy. Total RNA was isolated from the other two sample sets using rnaqueous MAG-96 and then converted to cDNA. Target cDNA levels were analyzed in duplicate using a TaqMan Gene Expression Assay for surviv...

Optimum FFPE RNA Isolation Kit

... was fixed in formalin for 24 hours and embedded in paraffin. Two, 10 m sections were used for RNA isolation. RNA was isolated using the rnaqueous Micro Kit for the frozen tissue and Optimum FFPE RNA Isolation Kit for FFPE tissue, respectively. ...

IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

...ells using siPORT Lipid at the final siRNA concentrations shown. Forty-eight hours after transfection, RNA was recovered using rnaqueous MAG-96 RNA Isolation Kit and reverse transcribed using the RETROscript Kit. Target cDNA levels were measured by real-time PCR an...

GENE SILENCING WITH PCR PRODUCTS

... SECs were transfected into 293 cells and RNA from the cells was analyzed after the indicated number of days. RNA was isolated with the rnaqueous Kit and then GAPDH mRNA levels were assessed by Northern blot using the NorthernMax-Gly Kit. The data are expressed as a percent...

Bacterial mRNA Isolation, Fast and Easy

...ess can be used with total RNA obtained by a variety of methods, including glass fiber filter-based methods (such as Ambion's rnaqueous Kits), and phenol-based methods (such as Ambion's RiboPure-Bacteria Kit). The mRNA isolated with MICROB Express is a superi...