Quantitation

ForteBio Launches New Biosensor for Kinetic Analysis and Screening of Human IgG Antibodies

...rther expands the capabilities of the Octet platform which already supports quantitation of human IgG using Protein A. ForteBio's Octet biosensors are configured in a standard 96-well format. Its biosensors simplify quantitation and kinetic characterization by eliminating throughput limitations of tradi...

Best New Product Awards Winners Named at Molecular Medicine Tri-Conference

... ClearPoint Peptides By spiking protein tryptic digests with internal heavy isotope labeled peptide standards, Mass Spectrometry becomes an absolute quantitation (AQUA) method. With a choice of heavy hydrogen (2H), carbon (13C), or nitrogen (15N)-isotopes specifically labeled at single, multiple or universal p...

USP announces revised glycerin monograph

...new procedure, Diethylene glycol and ethylene glycol impurities, capable of quantifying diethylene glycol and ethylene glycol at or above the Limit of quantitation (0.025%) is added. This diethylene glycol and ethylene glycol impurities test will be added to ascertain the level at which these impurities are prese...

Digirad to Introduce New Clinical Standard for Cardiac SPECT

...lf the acquisition time of traditional 2D approaches while providing reliable function quantitation measurements from gated stress images and reliable quantitation of perfusion-defect severity on stress and rest images. About Digirad Corporation Digirad provides diagnostic medical imaging systems and services...

Abbott's RealTime Hepatitis B Viral Load Test Receives CE Marking in Europe

... with Celera. The Abbott RealTime HBV test, developed for use on the Abbott m2000(TM) automated instrument system, is designed for the quantitation (precise measurement) of HBV in human plasma or serum from patients known to be infected with the virus. It is intended for use in conjunction with cl...

More Support for Zinc-antiatherogenic Link

... F(2)-isoprostane measurement. But since analysis of different biomarkers often requires different protocols, it adapted this technique to include the quantitation of cholesterol oxidation products in the same sample. Halliwell's team found that zinc supplementation did not have a significant impact on the...

Quantitation in Medical Technology

CRi Announces New Maestro(TM) EX In Vivo Imaging System with Improved Performance and an Affordable Price

...ing emission signals." explains James Mansfield, Director of Multispectral Imaging Systems at CRi. The Maestro EX enables more precise detection and quantitation of molecular probes in live animals and complements the automated high-throughput Maestro 2 imaging system, by providing comparable imaging performanc...

Final Results of Ideal Study Presented at Annual Meeting of the European Association for the Study of the Liver (EASL)

...4-week post- treatment assessment, the patient's 12-week post-treatment assessment was utilized. 3 Roche Cobas Taqman 1.0 assay; lower limit of quantitation (LLQ) is 27 IU/mL. 4 Intention-To-Treat (ITT) analysis includes any patient who has taken at least one dose of any study drug. 5 Sensitivity A...

IR BioSciences Announces Data Demonstrating Radilex Stimulation of Stem Cells Results in Hematopoietic Recovery Following Lethal Irradiation

...matopoietic mechanisms were nonetheless adequately stimulated to enable survival of a number of Radilex-treated, but no untreated, animals. Blood cell quantitation showed trends in the surviving animals toward neutrophil replenishment as well. ImmuneRegen has since filed a provisional patent entitled "Method to T...

Quantitation in Medical Products

Protein Electrophoresiswith Reflex to Immunofixation Electrophoresis Monoclonal Protein Detection, Quantitation & Characterization, IgA, IgG, & IgM, Serum

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

Immunofixation Electrophoresis Monoclonal Protein Detection Quantitation & Characterization SPE, IFE, IgA, IgG, IgM

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

HIV-1 RNA Quantitation by PCR

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

HIV-1 Ultrasensitive Quantitation by PCR

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

HIV-1 RNA Quantitation by bDNA

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

Herpesvirus 6 (HHV6) (A&B), Quantitation by PCR

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

EBVDNA Quantitation by PCR

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

EBVDNA Quantitation by PCR, Whole Blood

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

Cytomegalovirus DNA Quantitation by PCR

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

Methaqualone, Serum or Plasma, Quantitation

Description: ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories' client...
Company:ARUP Laboratories

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Quantitation in Biological News

American Chemical Society Weekly PressPac -- April 8, 2009

...r food-related bioterrorism. Ricin can be obtained easily and quickly causes death when inhaled or eaten in small amounts. There is no known antidote. quantitation of ricin also has medical applications, since ricin immunoconjugates have been used as anticancer agents. Although earlier researchers have developed ...

New method for detection of phosphoproteins reveals regulator of melanoma invasion

..."Our results revealed successful selection and sequencing of phosphopeptides in proteolytic digests without affinity enrichment, as well as label-free quantitation of regulated protein phosphorylation events," concludes Dr. Ahn. "Further, we demonstrated pathway-dependent phosphorylation of FAM129B and discovered...

Quantitation in Biological Technology

ForteBio Launches New Assay Capabilities for High-Sensitivity Protein Quantitation and Fragment Screening on Its Octet(R) Platform

MENLO PARK, Calif., May 27 /PRNewswire/ -- ForteBio(R), Inc., a leading supplier of label-free technology that accelerates the development of biotherapeutic and pharmaceutical products, today announced that it has launched two new assay modules - for high-sensitivity protein quantitation and fragm...

Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer

Gargi Choudhary, Tania A. Sasaki, and Julian J. Phillips, Thermo Electron Corporation, San Jose, CA Chromatography and Mass Spectrometry Application Report Introduction Ion trap mass spectrometry is widely recognized for solving analytical problems requi...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimiz...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION DNA measurements are important for many aspects of molecular biology. Of thespectrophotometric methods available, UV absorbance measurement at 260 nm isthe simpl...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

Evelyn McGown, Ph.D. and Michael Su, M.S. Molecular Devices Corporation, 8/00 INTRODUCTION Adenosine triphosphate (ATP) is present in all living cells. Because the level isstrictly-controlled, assay of ATP can be used as a indicator of viable cell numb...

Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)

By Yan Zhang., Molecular Devices Corporation, 1311 Orleans Dr., Sunnyvale, CA 94089. Introduction This application note describes how to use the OliGreen Quantitation Kit from Molecular Probes in the Gemini XS, Gemini EM and SpectraMax M2 mic...

Achieving Reliable Protein Quantitation Results Using ICAT Reagents, MALDITOF Mass Spectrometry, and Advanced Data Interpretation Tool

Overview A challenge of quantitative analysis of complex protein mixtures using isotope coded affinity tags is to correctly identify and obtain accurate quantitative information for ICAT reagent pairs on a spectrum generated by mass spectrometry. Because MALDI-TOF mass spectr...

Small-Molecule Quantitation Using Automated Single-Shot Averaging MALDI-TOF Mass Spectrometry

Overview Traditionally, MALDI-TOF mass spectrometry has been used very little for quantitative studies. Analysts have usually assumed that potential differences in ionization efficiency between analyte and internal standards and an inherent heterogeneity of the sample spot wo...

Separation and Quantitation of R and S Enantiomers using Normal Phase Chromatography on an API 3000 LC/MS/MS System

Purpose Normal phase chromatography can be used for the analysis of chiral compounds using the API 3000 LC/MS/MS System. Overview In recent years, pharmaceutical companies have placed greater importance on examining the stereoisomeric composition of dru...

A new LC/MS/MS Research Method for Rapid Quantitation of Five Immunosuppressant Drugs, including Mycophenolic Acid (MPA)

Purpose A previously published application note in this series describes an LC/MS/MS method for the simultaneous determination of four immunosuppressant drugs: Cyclosporin A (CsA), Tacrolimus (TAC, aka FK-506), Sirolimus (SIR, aka Rapamycin) and Everolimus (EVE, aka RAD-001) ...

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Quantitation in Biological Products

1-D PAGE Cleavable ICAT Reagent Applications Development Kit for Targeted Protein ID and Quantitation (monoplex Version) from Applied Biosystems

Description: The 1-D PAGE Cleavable ICAT Reagent Application Kit for Targeted Protein ID and Quantitation combines SDS PolyAcrylamide Gel Electrophoresis (PAGE), isotope-coded affinity tag chemistry, and cleavable-linker technology to facilitate the identification and quantification of differentially expressed ...
Company:Applied Biosystems

DNA Quantitation Kit (Hoechst) from Bio-Rad

Description: The DNA quantitation kit is used to quantitate double-stranded DNA (dsDNA) in solution, even in the presence of RNA or protein. With this kit, DNA is stained with Hoechst 33258 dye, which can be detected with a VersaFluor or other fluorometer. The kit quantitates dsDNA in the range of 10 ng per ml ...
Company:Bio-Rad

Quant-iT RiboGreen RNA Assay Kit from Molecular Probes (Invitrogen)

Description:... The RiboGreen RNA quantitation Kit is one of the most sensitive detection systems for the quantitation of RNA in solution. The assay is approximately 1000-fold more sensitive th...
Company:Molecular Probes (Invitrogen)

Phosphate Colorimetric Assay Kit, PiBlue™ from BioAssay Systems

Description:... assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. quantitation of phosphate content in protein, lipids, and DNA....
Company:BioAssay Systems

Phosphate Colorimetric Assay Kit from BioAssay Systems

Description:... assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. quantitation of phosphate content in protein, lipids, and DNA....
Company:BioAssay Systems

MEK1, total EIA Kit from Assay Designs/Stressgen Bioreagents

Description:... Fast, sensitive quantitation of total MEK 1/2 concentration....
Company:Assay Designs/Stressgen Bioreagents

Mouse IgM EIA Kit from Assay Designs/Stressgen Bioreagents

Description:... Fast, sensitive quantitation of Mouse IgM concentration....
Company:Assay Designs/Stressgen Bioreagents

CyScribe First-Strand cDNA Labeling System - dCTP with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description:...ied with over 99.9% removal of unincorporated CyDye label and primers, yielding superior cDNA purity.Complete protocols for labeling, purification and quantitation of CyDye la. Category: Microarrays, Target Preparation, Labeling....
Company:GE Healthcare, formerly Amersham Biosciences

CyScribe First-Strand cDNA Labeling Kit with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description:...ied with over 99.9% removal of unincorporated CyDye label and primers, yielding superior cDNA purity.Complete protocols for labeling, purification and quantitation of CyDye la. Category: Microarrays, Target Preparation, Labeling....
Company:GE Healthcare, formerly Amersham Biosciences

CyScribe First-Strand cDNA Labeling System - dUTP with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description:...ied with over 99.9% removal of unincorporated CyDye label and primers, yielding superior cDNA purity.Complete protocols for labeling, purification and quantitation of CyDye la. Category: Microarrays, Target Preparation, Labeling....
Company:GE Healthcare, formerly Amersham Biosciences

Quantitation in Biological Definition

Proteomics

...m previous estimates. Proteomic World - Resources for proteomics research. Yeast GFP Localization Database - Database of microscope images and quantitation for most of the yeast proteome. References Twyman, R. M. 2004. Principles of proteomics. BIOS Scientific Publishers, New York. ISBN 185996...

Quantitation in Biological Dictionary

SiRNA

... siRNA Synthesis. Premium quality siRNAs ... Made-to-order Silencer Select siRNA Libraries ... siRNA /Target Protein Isolation, Detection, & quantitation ... Predesigned siRNA and siRNA libraries designed to reduce off-target effects and increase RNAi performance. Custom siRNA synthesis service...

S1 nuclease

.... ... Nuclease Protection Assays (NPAs): The Basics covers the use of NPAs, including both RPAs and S1 nuclease assays, for the detection and quantitation of RNA. Topics ... If S1 nuclease can digest this single-strand loop and subsequently cleave the ... Moreover, S1 nuclease also cleaves he...

RT-PCR

...R , however, RNA... Eleven Golden Rules of Quantitative RT - PCR ". Plant Cell 20: 1736–1737. ... Type A influenza virus detection and quantitation by real-time RT - PCR " ... ... describes several factors that can dramatically affect the success of an RT - PCR procedure. ... The ...

Glycomics

...astardly, and Compelling ... Structural glycomics using hydrophilic interaction chromatography (HILIC) with ... Tools for glycomics : relative quantitation of glycans by isotopic permethylation ... Bioinformatics Support for Glycomics Research ... The Glycomics Ontology (GlycO) focuses on the glyco...

Antibody

... intact antibody can bind to ... Antibodies ... to which the antibody is conjugated; More ... Custom Antibody Production Services. Ig ELISA quantitation Kits. Immobilized E.coli Lysates ... YSK Antibody (C-term) Cell Function, YSK Antibody (C-term) Nuclear Signaling, ... This antibody is gener...