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QuantiTect in Biological Technology

FlexiPlate siRNA

...A Kits which are ideal for high-throughput screening. quantitect Primer Assays are ready-to-use primer sets for quantitative, r...le for every human and mouse gene. When used in combination with quantitect SYBR Green Kits, accurate and sensitive real-time RT-PCR is assu...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

...s. The PCR amplification of the cDNA was performed in a LightCycler instrument (Roche) in 20 l reactions using protocols and components of the quantitect SYBR Green PCR-Kit (Qiagen). cDNA-specific primers for murine PBGD (porphobilinogen deaminase) were used as model system producing a PCR-fragm...

TransMessenger Transfection Reagent Handbook

...monitored at the mRNA level by real-time RT-PCR. QIAGEN offers the quantitect SYBR Green RT-PCR Kit and the quantitect Probe RT-PCR Kit for highly sensitive and specific two-step and on...

QuantiTect Probe PCR Kit

... quantitect Probe PCR Master Mix Yes 2... * quantitect Probe Master Mix is a premixed solution containing HotStarTaq DNA Polymerase, quantitect Probe PCR Buffer, dNTPs (including dUTP for option...

Critical factors for successful microarray and real-time PCR analyses

... was used for quantitative RT-PCR using the quantitect Probe RT-PCR Kit and primers and pr...s quantitative, real-time RT-PCR. quantitect Kits from QIAGEN provide high sensitivity and spec... Quantitative real-time RT-PCR using the quantitect Probe RT-PCR Kit confirms the RNA...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

... formalin-fixed tissue). Real-time, quantitative RT-PCR was carried out on an ABI PRISM 7700 Sequence Detector using the quantitect Probe RT-PCR Kit and primers and probes for -actin or TNF-alpha, as indicated. All reactions were run in quadruplicate. ...

QuantiTect SYBR Green RT-PCR Kit

... In one-step quantification of RNA using the quantitect SYBR Green RT-PCR Kit, sensitivity is enh...gh Reproducibility and Sensitivity of the quantitect SYBR Green RT-PCR Kit "). ... High Reproducibility and Sensitivity of the quantitect SYBR Green RT-PCR Kit ...

Custom and library siRNA for efficient gene silencing

...evel of GAPDH (internal control), which was quantified by real-time RT-PCR using OmniscriptReverse Transcriptase and the quantitect SYBR Green PCR Kit. Expression levels were calculated using a standard curve generated using different amounts of HeLa S...

High-throughput gene expression analysis to screen for anti-asthma drugs

...ion. One-step, real-time RT-PCR using the quantitect Probe RT-PCR Kit provided highly sensitiv...n and real-time RT-PCR analysis using the quantitect Probe RT-PCR Kit enable sensitive analysi...I PRISM 7900HT Sequence Detection System using the quantitect Probe RT-PCR Kit. Res...

BioRobot MDx standardize your automated protocols in clinical laboratories

...evel of GAPDH (internal control), which was quantified by real-time RT-PCR using OmniscriptReverse Transcriptase and the quantitect SYBR Green PCR Kit. Expression levels were calculated using a standard curve generated using different amounts of HeLa S...
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(Date:4/17/2014)... April 2014) Population Council scientists and their partners have ... and can prevent the transmission of multiple sexually transmitted ... animals: HIV, herpes simplex virus 2 (HSV-2), and human ... data that the gel is effective against multiple strains ... the vagina against all three viruses of at least ...
(Date:4/17/2014)... less than 40 years away from a food shortage ... according to a top scientist at the U.S. Agency ... history, food production will be limited on a global ... said Dr. Fred Davies, senior science advisor for the ... as politically destabilizing by 2050 as energy issues are ...
(Date:4/17/2014)... work has transformed cancer care in Northern Ireland, has ... of Cancer Sciences., The Academy, which was launched in ... oncologists and cancer researchers which aims to reduce the ... Professor Johnston, whose leadership has seen cancer survival rates ... UK league table to near the top, has been ...
(Date:4/16/2014)... PHOENIX, Ariz. April 15, 2014 The Translational ... philanthropists at their annual Founders Dinner for their support ... The event took place March 28 in Scottsdale. , ... Catherine Ivy Foundation, received TGen,s John S. McCain Leadership ...
(Date:4/16/2014)... fertility treatments are the leading cause of increases in ... of premature birth, these results are not inevitable, concludes ... . The article identifies six changes in policy and ... and prematurity, including expanding insurance coverage for in vitro ...
Breaking Medicine News(10 mins):Health News:Animal study provides first evidence that gel can prevent multiple virus transmission in vagina/rectum 2Health News:Food shortages could be most critical world issue by mid-century 2Health News:TGen honors Catherine Ivy and Craig Jackson with Leadership; Collaborative Spirit awards 2Health News:Preterm births, multiples, and fertility treatment 2Health News:Preterm births, multiples, and fertility treatment 3
(Date:4/16/2014)... , A 20-year assessment of Nicaragua,s legal, artisanal green ... reduced overall catch rates of turtles in what may ... from the Wildlife Conservation Society and University of Florida. ... more than 170,000 green turtles were killed between 1991 ...
(Date:4/16/2014)... away, the top of a leaf looks like one ... actually made up of a patchwork of cells in ... these cells individually take on their own unique forms, ... colleagues sought to pinpoint the shape-controlling factors in pavement ...
(Date:4/16/2014)... and bulbar muscular atrophy (SBMA) is a rare inherited neuromuscular ... have long considered it to be essentially an affliction of ... and brainstem that control muscle movement. , But in a ... of Neuron , a team of scientists at the ...
Breaking Biology News(10 mins):Declining catch rates in Caribbean green turtle fishery may be result of overfishing 2Declining catch rates in Caribbean green turtle fishery may be result of overfishing 3For cells, internal stress leads to unique shapes 2Mutant protein in muscle linked to neuromuscular disorder 2
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