QuantiTect

FlexiPlate siRNA

...A Kits which are ideal for high-throughput screening. quantitect Primer Assays are ready-to-use primer sets for quantitative, r...le for every human and mouse gene. When used in combination with quantitect SYBR Green Kits, accurate and sensitive real-time RT-PCR is assu...

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

...s. The PCR amplification of the cDNA was performed in a LightCycler instrument (Roche) in 20 l reactions using protocols and components of the quantitect SYBR Green PCR-Kit (Qiagen). cDNA-specific primers for murine PBGD (porphobilinogen deaminase) were used as model system producing a PCR-fragm...

TransMessenger Transfection Reagent Handbook

...monitored at the mRNA level by real-time RT-PCR. QIAGEN offers the quantitect SYBR Green RT-PCR Kit and the quantitect Probe RT-PCR Kit for highly sensitive and specific two-step and on...

QuantiTect Probe PCR Kit

... quantitect Probe PCR Master Mix Yes 2... * quantitect Probe Master Mix is a premixed solution containing HotStarTaq DNA Polymerase, quantitect Probe PCR Buffer, dNTPs (including dUTP for option...

Critical factors for successful microarray and real-time PCR analyses

... was used for quantitative RT-PCR using the quantitect Probe RT-PCR Kit and primers and pr...s quantitative, real-time RT-PCR. quantitect Kits from QIAGEN provide high sensitivity and spec... Quantitative real-time RT-PCR using the quantitect Probe RT-PCR Kit confirms the RNA...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

... formalin-fixed tissue). Real-time, quantitative RT-PCR was carried out on an ABI PRISM 7700 Sequence Detector using the quantitect Probe RT-PCR Kit and primers and probes for -actin or TNF-alpha, as indicated. All reactions were run in quadruplicate. ...

QuantiTect SYBR Green RT-PCR Kit

... In one-step quantification of RNA using the quantitect SYBR Green RT-PCR Kit, sensitivity is enh...gh Reproducibility and Sensitivity of the quantitect SYBR Green RT-PCR Kit "). ... High Reproducibility and Sensitivity of the quantitect SYBR Green RT-PCR Kit ...

Custom and library siRNA for efficient gene silencing

...evel of GAPDH (internal control), which was quantified by real-time RT-PCR using OmniscriptReverse Transcriptase and the quantitect SYBR Green PCR Kit. Expression levels were calculated using a standard curve generated using different amounts of HeLa S...

High-throughput gene expression analysis to screen for anti-asthma drugs

...ion. One-step, real-time RT-PCR using the quantitect Probe RT-PCR Kit provided highly sensitiv...n and real-time RT-PCR analysis using the quantitect Probe RT-PCR Kit enable sensitive analysi...I PRISM 7900HT Sequence Detection System using the quantitect Probe RT-PCR Kit. Res...

BioRobot MDx standardize your automated protocols in clinical laboratories

...evel of GAPDH (internal control), which was quantified by real-time RT-PCR using OmniscriptReverse Transcriptase and the quantitect SYBR Green PCR Kit. Expression levels were calculated using a standard curve generated using different amounts of HeLa S...