Real-Time Immuno-PCR on the iCycler iQ System, Rev A
... forward primer (biotin-AACTATGCCGGAATCCATCG- 3') and unmodified reverse primer (5'-ACATATCCAGCCATGCACAC- 3'), and was purified with the qiaquick PCR purification kit (QIAGEN). Real-time PCR was performed in the Bio-Rad iCycler iQ system. The primers used were 5'-...Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
...ed tissues can be significantly improved by cleanup with the qiaquick PCR Purification Kit, as described in the optimized protocol...EN protocol, either with (+) or without () cleanup using the qiaquick PCR Purification Kit. PCR of the GAPDH gene was carried out ...Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
...0 (QIAGEN, Santa Clarita, CA) using the qiaquick 96 PCR BioRobot Kit. The yield of PCR amplicon ...l of 5N HCl, and cDNA probes were purified using a qiaquick PCR purification kit (Qiagen, Santa Cla...000 (QIAGEN, Santa Clarita, CA) using the qiaquick 96 PCR BioRobot Kit. Representative DNA s...High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
...ed with either the StrataPrep 96 PCR purification kit or Qiagens qiaquick 96 PCR purification kit. The purified samples were sequenced wit...ity data with no ambiguities in over 500 bases. In contrast, the qiaquick 96 PCR kit demonstrated sequence data that had high background, ...