Transfection Reagent Selector Kit Handbook
...sed for transfection. DNA purified with QIAGEN and
qiafilter Plasmid
Kits is ideally suited for transf... should be purified using QIAGEN
or
qiafilter Plasmid Kits or an equivalent method. For endotoxi...uld be
used. DNA purified with QIAGEN and
qiafilter Plasmid Kits is ideally suited
for transf...
QIAfilter Plasmid Kits
...For purification of low-copy plasmids and cosmids,
qiafilter Plasmid
Mega Kits are a better choice than
qiafilter Plasmid Giga Kits, due to the
large cultu...e volumes required and the limited capacity of the
qiafilter
Mega-Giga Cartridge.
...
EndoFree Plasmid Kits
...e volumes required and the limited capacity of the
qiafilter
Mega-Giga Cartridge
B...llowing clearing of the bacterial lysate using the
qiafilter Cartridge
(see "
qiafilter
principle " ), the Endotoxin Removal Bu...
HiSpeed Plasmid Kits
...
qiafilter Cartridges are special filter units
... alkaline lysis
of bacterial cells.
qiafilter Cartridges completely remove SDS precipitates
...Neutralized bacterial lysates are incubated in the
qiafilter Cartridge and
cleared in seconds by filtr...
Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell
Line
...lar
biology applications.
Four different plasmid purification methods were analyzed. Stratagene's
StrataPrep EF plasmid midiprep kit, Qiagen's
qiafilter Plasmid Maxi kit with
additional Endotoxin Free Buffer set (AEC: anion exchange chromotography),
Promega's Wizard PureFection Plasmid DNA Purifi...