Pwo Master*: Simplify Setup of Your Proofreading PCRs

... With the introduction of the pwo Master, Roche Applied Science now offers a new, ...CR synthesis. pwo Master is a premixed double-concentrated formulati... to 50 l with PCR-Grade Water (provided with the pwo Master), the reaction can be started. ...

Pwo DNA Polymerase

... Description pwo DNA polymerase was originally isolated from hypert...nzyme has no detectable 5'3' exonuclease activity. pwo DNA polymerase exhibits increased therm... 3'5' exonuclease proofreading activity pwo results in an over 18-fold increased fidelity of D...

High Fidelity PCR Master

...cessary reagents (buffers, nucleotides and enzymes) needed for PCR. The mix contains a unique enzyme blend of Taq DNA Polymerase and pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity]. Due to the inherent 3'5' exonuclease proofrea...

Expand Long Template PCR System

... Description Unique enzyme blend of Taq and pwo DNA Polymerase. The system also includes PCR buffers for optimal a... PCR System enzyme blend combines the proofreading activity of pwo DNA Polymerase with the high processivity of Taq DNA Polymerase ...

Expand 20 kbPLUS PCR System

...enomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kb PLUS PCR System also includes an optimized reaction buffer, MgCl...

Product overview

... a unique enzyme blend containing Taq and pwo DNA Polymerase and another thermostable enzyme. ...ue enzyme blend of Taq DNA Polymerase and pwo DNA Polymerase[a thermostable enzyme with a proofr...A Polymerase. pwo DNA Polymerase ...

Expand High Fidelity PCR System

... Description Unique enzyme blend of Taq and pwo DNA Polymerases. The Expand High Fidelity PCR System also includes... Expand High Fidelity PCR System is an unique enzyme blend (Taq / pwo DNA polymerase) that is ideal for amplifying fragments up to 5 k...

GC-RICH PCR System

...306 50 PCR reactions Description Unique enzyme blend of Taq DNA Polymerase and pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity]. The GC-RICH PCR System also includes: ...

Comparing Fidelity and Performance of Proofreading PCR Enzymes

... DNA polymerase, cloned Pfu DNA polymerase (without enhancer), and other Pfu-related proofreading enzymes, including Vent , Deep Vent , KOD, and pwo DNA polymerases. 4,5 A competitor recently introduced a new proofreading PCR enzyme, called PLATINUM Pfx DNA polymerase. Contrary to its trade ...

Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

...equired 1.25 U to obtain sufficient yields with minimal smearing. Higher yields of PCR products were obtained when 5 U of Pfu, pwo or Taq DNA polymerase was used (data not shown). Smearing or reduced yield was observed when higher amounts of KOD, Vent R or...

Pwo in Biological Products

Expand 20 kb ^PLUS PCR System; Thermostable DNA polymerase mixture from Roche Applied Science

Description:... Unique enzyme blend containing Taq and pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kbPLUS PCR System also includes an optimized reaction buffer, MgCl2 solution, and a huma...
Company:Roche Applied Science

Pwo in Biological Definition

Polymerase chain reaction

...when copying DNA, leading to mutations (errors) in the DNA sequence, since it lacks 3'->5' proofreading exonuclease activity. Polymerases such as pwo or Pfu, obtained from Archaea , have proofreading mechanisms (mechanisms that check for errors) and can significantly reduce the number of mut...