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Puromycin in Biological Technology

Enhanced siRNA Delivery and Long-term Gene Silencing

... (A) Infected HeLa cells were placed under puromycin selection (4 days). After two months of...e placed under selection with puromycin at 1, 1.5, and 2 g/ml, respectively. A control ... HeLa cells. The HeLa cells were placed under puromycin selection (4 days). (A) Cells were peri...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...ein were readily detected in the transfected cells 24 or 48 hours after transfection. The presence of a hygromycin, neomycin, or puromycin antibiotic resistance genes on each p Silencer 4.1-CMV vector further allows for selection of stable cell lines constitutively ...

Five Ways to Produce siRNAs

... and are designed especially for subcloning SECs, are available separately. These ready-to-ligate vectors include a hygromycin, neomycin or puromycin resistance gene to permit selection in mammalian cells. Figure 4. Variable Reduction in Target Gene Expr...

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

...essing hrGFP as the reporter; detecting transfected cells via expression of hrGFP reporter; exchanging hygromycin, neomycin, and puromycin drug-resistance genes by Cre-Lox recombination; confirming transfection efficiency, and detecting fluorescence in vivo with the ...

Safe Production of High-Titer Retrovirus

... 7 g/ml of puromycin. They showed a high level of colony formation above untransfected CHO cells, which showed no colony formation at all with the puromycin concentrations tested. The pVPack-VSV-G vector was not tested for stable cell formation due to reported toxicity of the VSV-G envelope protein to ...
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