Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses
...roporation pulses generated by the Gene pulser II system for transient gene expression in COS c...ansient expression was obtained with both the Gene pulser II system and the EasyJect Plus with a ...sing single pulses produced by the Gene pulser II system were performed with either low voltage a...Transgenic Zebrafish by Electroporation
...yos is tedious and time consuming. We have used the Bio-Rad Gene pulser apparatus to generate transgenic fish by electroporation. To e...os are pulsed three times using the 0.25 F capacitor of the Gene pulser apparatus (Bio-Rad). The pulse field strength is 125 V/cm, and e...Electroporation of Yeast Artificial Chromosomes
...ed with 1-5 l of DNA (0.1 g) just before electroporation. A Gene pulser apparatus connected to the Pulse Controller accessory was used...hance to enter the cell. Lower voltage experiments with the Gene pulser apparatus (which generates an exponential decay pulse) have a le...Electroporation of Primary Bone Marrow Cells
...cells using electroporation. We have used the Gene pulser apparatus to electro-transfect either...mples of 800 l each were placed in 0.4 cm gap Gene pulser cuvettes and 10 l of DNA (1.0 g/ml) add...X, when 500 F and 300 V (750 V/cm) Gene pulser settings were used and a pulse time constant of ...High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
...oblastsNeonatal (an adherent primary cell type) using the siPORTer-96 Electroporation Chamber (Ambion) powered by a Bio-Rad Gene pulser Xcell pulse generator. Samples 148 (shown) were transfected with Silencer GAPDH siRNA (Ambion) while samples 4996 were transfe...Deliver siRNAs Into Primary Cells
...RNA Electroporation Kit has been tested with two commonly used single-cuvette electro-pulse generators: Gene pulser Xcell (Bio-Rad) and ECM 830 (BTX). Their performance was similar when the same electroporation parameters we...High Throughput siRNA Electroporation
... Use with Bio-Rad Gene pulser Xcell pulse generator Ele...; Figure 1) works with the Bio-Rad Gene pulser Xcell pulse generator to allow high throughput siR... When used with Bio-Rad's Gene pulser Xcell pulse generator, Ambion...Delivering siRNAs to Difficult Cell Types
...rmed with two different electro-pulse generators: Gene pulser Xcell (Bio-Rad) and ECM 830 (BTX). Both electroporator...xpression (variability <10%; data not shown). The Gene pulser Xcell System performed very consistently without sampl...Gene Pulser/E. coli Pulser Cuvette Rack from Bio-Rad
Description: The expertise that made the original Gene Pulser apparatus and accessory modules the most popular electroporation system available has been applied in the Gene Pulser II system, which features unique PulseTrac circuitry. The modular design of the Gene Pulser II system has the dual advantages of usi...Gene Pulser Xcell Microbial System from Bio-Rad
Description: The Gene Pulser Xcell microbial system, 100 to 240 V, 50-60 Hz, is used for electroporation of bacterial and fungal cells. This system includes the main unit, PC module for exponential decay delivery, ShockPod shocking chamber, 10 sterile cuvettes (5 each of 0.1 and 0.2 cm gap), cuvette rack, and i...Gene Pulser Xcell Eukaryotic System from Bio-Rad
Description: The Gene Pulser Xcell eukaryotic system, 100 to 240 V, 50-60 Hz, is used for electroporation of mammalian cells. This system includes the main unit, CE module for exponential (25-3,275 microfarad range) or square-wave pulses, ShockPod shocking chamber, 5 sterile cuvettes (0.4 cm gap), and instructi...siPORTer™-96 Electroporation Chamber from Ambion
Description:...hour from cell harvest to post-electroporation incubation Highly reproducible results from well-to-well Designed for use with the Bio-Rad Gene pulser Xcell System ...