Less is more, gene study shows
...have a working copy.
"This is another indication that loss of the gene would be good for the individual, but it doesn't show that the gene became a pseudogene
because of that advantage." However, using population genetics techniques, the researchers were able to demonstrate just that. They also determined wh...
Cats' indifference towards sugar explained
...ing to Brand, Xia Li (lead author of the study), and their coauthors, cats do not produce one of the proteins, because the gene that codes for it is a pseudogene
and is not functional. It is this lack of a functional sweet receptor that explains cats' indifference to sweet stimuli.
Cats have no way to recogn...
Pseudogene in Biological Technology
DNA-free A NEW Method to Remove DNA
...ouse liver RNA (0.5 g) was used in RT-PCR. The
S15 PCE primers span an intron-exon junction. Products from both the gene
and the pseudogene
for this message are detected in the minus-RT reaction.
Getting Rid of Contaminating DNA and the DNase Used
QIAGEN Multiplex PCR Handbook
Primers may anneal to pseudogene
sequences and additional
PCR products may be amplified. Revi...
Fast Isolation of Total RNA from Small Numbers of Cells
... 8 The Mx4000 molecular
beacon GAPDH expression analysis kit specifically detects GAPDH mRNA sequences
but not genomic DNA or pseudogene
sequences. 9 Detecting
as little as 1 pg of human total RNA or 0.01 pg of poly(A) +
RNA is possible with this kit. 10 Signal ...
High-Throughput Isolation of Total RNA
... RNA samples isolated with both kits using primers
specific for GAPDH. These primers amplify a 209-bp product from cDNA and
sequences in genomic DNA. We performed cDNA synthesis
reactions both in the presence and absence of reverse transcriptase, followed
Pseudogene in Biological Definition
...py and now the other gene locus is free to mutate and discover new functions. Alternatively, the gene may acquire deleterious mutations and become a pseudogene
The postulate that gene duplication has a major role in evolution was developed in the 1980s and is now widely accepted as a major evolutionary...
...f the new variant remains identical to the original, then it is likely that one or the other will be lost as mutations accumulate, resulting in a pseudogene
. However, if the mutations do not immediately prevent the enzyme from functioning, but instead modify either its function, or its pattern of gene ex...
Pseudogene in Biological Dictionary
structure in 5S DNA of Xenopus laevis" ... The putatively functional Mkrn1-p1 pseudogene
is neither expressed nor imprinted, ... Welcome to Pseudogene
.org. The site is developed and maintained by Yale Gerstein Group. ... Quick Links. Human Pseudogene
Sets. ENCODE ...