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Propidium in Medical News

A potential drug for liver carcinoma

...the inhibitory effect of ADFMChR on their proliferation was measured by MTT assay. The apoptosis of HepG2 cells was determined by flow cytometry using propidium iodide fluorescence staining. The influence of ADFMChR on the proxisome proliferator-activated receptor γ (PPARγ), NF-κB, Bcl-2 and Bax...
Propidium in Biological Technology

Inoculation, Growth & Harvesting in a New Easy-to-Use Disposable Cell Culture System

...cartridge the NucleoCassette , which contains a nucleus staining dye, propidium iodide. To use, aseptically remove a few samples of the FibraCel disks an...defined volume of your sample into the cassette. The sample dissolves the propidium iodide dye, which has been immobilized inside the cassette. The cassett...

New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting

...he small, plastic cassette is internally pre-coated with a fluorescent dye, propidium iodide (PI), which stains the cells' nuclei. Because the NucleoCounter dete...surement chamber using fluorescence microscopy with appropriate filters for propidium iodide. Clean up is simple. After use, the NucleoCassette can be ...

Improving the accuracy and speed of mammalian cell counting

... fluorescence microscope designed to detect signals from a fluorescent dye, propidium iodide (PI), which intercalates to DNA in the cell nuclei (figure 2). Excit...s then placed into the NucleoCounter for analysis. The lysate dissolves the propidium Iodide, which is immobilized in the cassette, and a mechanical drive in the...

Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells

...itors in triplicate using siPORT NeoFX Transfection Agent (Ambion). 72 hr post-transfection, cells were fixed and stained with propidium iodide to count total cell number (TTP LabTech Acumen Exporer). Cells were then stained for -actin using immunofluorescence. ...

B16

...ion: The expression of the plasmid pEGFP-N1 by viable B16 cells can be detected after 24-48 hours with a flow cytometer by costaining with propidium iodide (final concentration 1 g/ml). Incubate for 10 min. at RT. Result: ...

721.82

...The expression of the plasmid pEGFP-N1 by viable 721.82 cells can be detected after 24 hours with a flow cytometer by costaining with propidium iodide (final concentration 1 g/ml). Incubate for 10 min. at RT. Result: ...

721.221

...he expression of the plasmid pEGFP-N1 by viable 721.221 cells can be detected after 24 hours with a flow cytometer by costaining with propidium iodide (final concentration 1 g/ml). Incubate for 10 min. at RT. Result: ...

721.174

...he expression of the plasmid pEGFP-N1 by viable 721.174 cells can be detected after 24 hours with a flow cytometer by costaining with propidium iodide (final concentration 1 g/ml). Incubate for 10 min. at RT. Result: ...

Optimizing electroporation parameters

...ng so is by incubating the cells for 30 minutes in hypoosmolar buffer and then performing a viability stain using trypan blue or propidium iodide. If observation under a microscope reveals lysis in more than 10% of the cells, the osmolarity of the buffer must be incr...

Reliable Assessment of Micronuclei for Genotoxicity Testing in Mice or Rats

...en fixed blood cells are treated with ribonuclease and antitransferrin receptor antibody (FITC-conjugate), and then stained with propidium iodide, normochromatic erythrocytes (NCEs) and RETswith and without MNare clearly resolved ( Figure 1 ). ...
Propidium in Biological Products

propidium iodide *FluoroPure™ grade* from Molecular Probes (Invitrogen)

Description:... Because it is manufactured at our ISO 9001–certified facilities in Eugene, Oregon, we can guarantee that FluoroPure grade propidium iodide is greater than or equal to 98% pure by HPLC....
Company:Molecular Probes (Invitrogen)

Vybrant Apoptosis Assay Kit #4 from Molecular Probes (Invitrogen)

Description:...ll membranes. This kit contains ready-to-use solutions of both YO-PRO-1 and propidium iodide nucleic acid stains. YO-PRO-1 stain selectively passes through the p...oderate green fluorescence. Necrotic cells are stained red-fluorescent with propidium iodide....
Company:Molecular Probes (Invitrogen)

Propidium Iodide Solution [3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide] from CHEMICON

Description:... {3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide}. 250 ug/ml propidium Iodide (PI) in PBS....
Company:CHEMICON

Propidium Iodide/Antifade Solution from CHEMICON

Description:... propidium Iodide: {3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthrid...ntifade Solution: p-Phenylenediamine and glycerol in PBS solution. 2.5ug/mL propidium Iodide (PI) suspended in Antifade....
Company:CHEMICON

Propidium Iodide from GeneTex

Description:... Concentration: 1mg/ml. Antigen: propidium Iodide ...
Company:GeneTex

Flow Cytometry Kit for Apoptosis from Sigma-Aldrich

Description:...on: The greater incorporation of Br-dUTP results in a stronger signal by flow cytometry when detected using a fluorescein-labeled anti-BrdU antibody. propidium iodide/RNase A solution is included in the kit to counterstain the total DNA. Features and Benefits: Greater incorporation of Br-dUTP, resulting in im...
Company:Sigma-Aldrich

Propidium Iodide from KPL

Description:... propidium Iodide (PI) is a fluorescent nucleic acid dye whic...tranded nucleic acids. As a nuclear counterstain, propidium iodide stains the nucleus light to dark red in col... or blue fluorochromes such as FITC, Cy2 or AMCA. propidium Iodide can be used for fluorescent in situ hybridi...
Company:KPL

FluoroGuard Antifade Reagent from Bio-Rad

Description:...ely suppresses photobleaching in a wide variety of fixed specimens stained or labeled with fluorescein, rhodamine, sulforhodamine 101, coumarin, DAPI, propidium iodide, and other fluorescent dyes....
Company:Bio-Rad

Propidium Iodide Solution from Calbiochem

Description:... Liquid. 50 μg/ml in PBS, pH 7.4. PROTECT FROM LIGHT. A convenient form of propidium iodide useful for flow cytometry studies. RTECS SF7949600, CAS 25535-16-4, M.W. 668.4. ...
Company:Calbiochem

Apo-BrdU Apoptosis Detection Kit from eBioscience

Description:...nucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), and fluorescein labeled anti-BrdU antibody for labeling DNA breaks and propidium iodide/RNase A solution for counterstaining the total DNA. One of the most easily measured features of apoptotic cells is the break-up of the genomi...
Company:eBioscience

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