Scientists explore factors contributing to DNA mutations
...peats indicate a lower repeat number and mutability is lower for microsatellites with lower repeat numbers. At shorter lengths, explained Makova, DNA proofreading
and repair mechanisms function more reliably and efficiently.
The team also discovered that repeat composition was a significant predictor of micro...
Proofreading in Biological News
Molecular typesetting -- proofreading without a proofreader
Researchers at the Universities of Leeds and Bristol (UK) have developed a model of how errors are corrected whilst proteins are being built.
Ensuring that proteins are built correctly is essential to the proper functioning of our bodies, but the 'quality assurance' mechanisms that take place d...
Elderly mice yield clues to the process of growing old
... spellchecker," says Prolla. "By introducing a malfunction in the (genetic) proofreading
domain, these mutations accumulate much more rapidly."
The new work lend...ipulating mouse embryonic stem cells to produce mice with the defective DNA proofreading
protein. The mice develop normally, but age rapidly and develop such things...
To Stop Evolution: New Way Of Fighting Antibiotic Resistance Demonstrated By Scripps Scientists
...es extremely well, and the "polymerase" enzymes that replicate our DNA rarely make mistakes. Even when they do, we have multiple, redundant repair and proofreading
mechanisms that would make even the most six-sigma-compliant NASA engineer jealous.
Nevertheless, all organisms are prone to some level of damage b...
Proofreading in Biological Technology
Simplify Setup of Your Proofreading PCRs
With the introduction of the Pwo Master, Roche Applied
Science now offers a new, ready-to-use proofreading
master mix for the robust amplification of fragments up to
3 kb, providing the highest possible fidelity during PC...
Comparing Fidelity and Performance of Proofreading PCR Enzymes
PfuTurbo DNA polymerase is superior to
Platinum Pfx DNA polymerase
Michael Borns Janice Cline Holly Hogrefe
We recently evaluated the properties of Platinum
Pfx DNA polymerase, a competitors new PCR enzyme formulation
Global Vision Recognized for Helping Pharmaceutical Companies Achieve Complete Artwork and Copy Accuracy in their Packaging Process
MONTREAL, Oct. 22 /PRNewswire/ -- Global Vision, the leader in
solutions for the pharmaceutical industry announced
today that 15 of the to...t 11 requirements.
About Global Vision
Global Vision provides automated proofreading
solutions that enable
organizations to achieve unprecedented content integr...
QIAGEN LongRange PCR and 2Step RT-PCR Kits
...th enhanced processivity, allows the amplification
of full-length PCR products. The enzyme mix provides high extension rates
ability for increased fidelity (up to 5-fold higher fidelity
than Taq DNA polymerase). The novel buffer system provides highly
GenomiPhi amplification of FFPE tissue DNA for array CGH
... (4). This method employs the unique biochemical properties of Phi29 DNA polymerase, a highly processive enzyme with excellent strand displacement and proofreading
ability, to amplify DNA.
In this application note, we show array CGH data on DNA isolated from human liver FFPE slides, comparing DOP-PCR wi...
Reduce Errors and Save Time When Setting Up PCRs
...nal concentration of
1.5 mM MgCl2. The Expand High Fidelity Enzyme blend is
a blend of Taq DNA Polymerase and a thermostable
DNA polymerase with proofreading
activity. This powerful
enzyme blend is designed to amplify any kind of DNA
up to 5 kb (Figure 1) with higher yield and three times
Roche Diagnostics Corporation
...f Beer Screening Kit
Screening of Beer-Spoilage Bacteria Using the LightCycler PCR Workflow System
Pwo Master*: Simplify Setup of Your proofreading
The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
Challenge the Performance of Your Hot-Start PCRs
with FastStart Taq DNA Polymerase and
the Novel FastStart High Fidelity PCR System
... FastStart Taq DNA Polymerase and a
protein that mediates proofreading
activity, but...Start Taq DNA Polymerase, and the ability
of the proofreading
protein to recognize and excise
incorrectly inco...ificity, sensitivity, and
yield (Figure 4).
polymerases or chemical modifications
PCR reaction components
...enzyme blends (e.g. the Expand
PCR Systems which contain blends of Taq DNA polymerase and a
thermostable polymerase with proofreading
To improve PCR yield, use the
Replacing a single polymerase with
a blend of a thermostable polymerase (Taq DNA Polymerase) and a proofreading
polymerase (Pwo DNA Polymerase) made PCR an indispensable tool in
the analysis and mapping of entire genomes by:
Proofreading in Biological Products
Zero Blunt TOPO PCR Cloning Kit with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen
Description:...The Zero Blunt TOPO PCR Cloning Kit offers the fastest and easiest method for high-efficiency (>=95%) cloning of blunt-end PCR products amplified with proofreading
thermostable polymerases. The kit includes the linearized and topoisomerase I-activated pCR-Blunt II-TOPO vector for 5-minute bench-top ligations with...
Zero Blunt TOPO Kit for Sequencing with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen
Description:...3-T overhangs for direct ligation of Taq-amplified PCR products. The pCR4Blunt-TOPO vector provides blunt ends for cloning PCR products amplified with proofreading
thermostable polymerases. Each vector is supplied linearized and topoisomerase I-activated enabling 5-minute bench-top ligation yielding recombinants ...
Zero Blunt TOPO PCR Cloning Kit with One Shot TOP10 Chemically Competent E. coli from Invitrogen
Description:...R Cloning Kit offers the fastest and easiest method for high-efficiency (greater than or equal to95%) cloning of blunt-end PCR products amplified with proofreading
thermostable polymerases. The kit includes the linearized and topoisom...
MasterAmp Taq DNA Polymerase Kit from EPICENTRE Biotechnologies
Description:... PCR and remains the predominant enzyme for that purpose. The enzyme has an intrinsic 5'?3' structure-dependent exonuclease activity, but lacks 3'--5' proofreading
exonuclease activity. MasterAmp Taq DNA Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial ...
AccuPrime Pfx from Invitrogen
Description:...cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Pyrococcus sp. with proofreading
(3'Cut Site5' exonuclease) activity. Platinum Anti-Pfx DNA Polymerase antibodies inhibit polymerase activity providing hot-start capabilities. Thermo...
Six Paq, rTth DNA Polymerase, XL and XL Buffer II Pack from Applied Biosystems
Description:... 6 x Blend of rTth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 proofreading
GC-RICH PCR System from Roche Applied Science
Description:... For amplification of GC-rich nucleic acids
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading
polymerase, powers through templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced ...
Company:Roche Applied Science
Expand High Fidelity PLUS PCR System; Thermostable DNA polymerase mixture from Roche Applied Science
Description:...new blend combines Taq DNA Polymerase with a novel proofreading
protein isolated and characterized by Roche Applied Science. This protein mediates proofreading
activity, but has no polymerase activity itself. The synergy between the mechanism of the proofreading
protein and the processivity of Taq DNA Polymerase...
Company:Roche Applied Science
Extensor Hi-Fidelity PCR Enzyme from ABgene
Description:... Synergistic enzyme mix (Thermoprime Plus DNA Polymerase with a proprietary thermostable proofreading
At least four times higher fidelity than standard Taq DNA polymerase
Double the yields of standard Taq DNA polymerase
Amplifies DNA templat...
GCpro Taq DNA Polymerase Complete System from GenScript Corporation
Description:...ining the DNA polymerase I gene from Thermococcus litoralis. The enzyme includes a highly processive 5'-3' DNA polymerase activity. Its inherent 3'-5' proofreading
activity results in a greatly increased fidelity of DNA synthesis compared to Taq DNA Polymerase. The enzyme includes an optimized reaction buffer, wh...
Proofreading in Biological Definition
Polymerase chain reaction
...eading to mutations (errors) in the DNA sequence, since it lacks 3'->5' proofreading
exonuclease activity. Polymerases such as Pwo or Pfu, obtained from Archaea , have proofreading
mechanisms (mechanisms that check for errors) and can significantly reduce...
Proofreading in Biological Dictionary
...n order to detect and correct any errors. Modern proofreading
often requires reading copy at earlier stages as well. Professional document editing and proofreading
services. Specializes in large projects and docume...larship each year. ... Proof - Reading .com Blog. proofreading
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