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Prokaryotic in Medical News

J. Craig Venter Institute Awarded $43 Million, Five Year Contract From NIAID to Continue to Develop and Provide Sequencing, Genotyping, and Bioinformatics Expertise and Services in Infectious Diseases

...t was previously referred to as the NIAID Microbial Genome Centers program. During that contract period JCVI sequenced and analyzed 185 eukaryotic and prokaryotic genomes, and more than 3000 viral genomes. JCVI's participation in the NIH Human Microbiome program was also initiated under this contract program. ...

Dermaseptins: Potent vaginal contraceptives

...ts can be used vaginally in effective doses without causing overt vaginal irritation or other toxicity. Antimicrobial peptides are produced by both prokaryotic and eukaryotic cells and have been the subject of many studies in recent years because of their involvement in host defense mechanisms and their poten...
Prokaryotic in Biological News

Spontaneous assembly

...ent. However, there is also complex spatial organization to be found within prokaryotic cells, such as rod-shaped bacteria like E. coli . "It has remained some...keletal involvement or active transport, may prove to be widespread in both prokaryotic and eukaryotic cells." Liphardt and his research group are now applying ...

JCVI program trains USDA scientists on eukaryotic genome analysis

... a deeper understanding of the annotations available in publicly accessible databases. In addition to the training, JCVI offers free annotation of prokaryotic genomes through the JCVI Annotation Service. This service has provided more than 100 investigators with more than 200 genomic sequences. Accordin...

Research-based undergraduate course expands beyond Washington University

...ing on the challenge of working with eukaryotic genomes. These genomes are more complex and orders of magnitude larger than the more commonly studied prokaryotic (for example, bacterial) genomes. The development of a coordinated research effort that pools student contributions across institutions allows the GE...

Researchers develop new self-training gene prediction program for fungi

...the first completely sequenced genomes of bacteria and archea. The research team then developed self-training versions of the gene finding program for prokaryotic (organisms that lack a cell nucleus) and eukaryotic (organisms that contain a cell nucleus) genomes in 2001 and 2005, respectively. Development of the...

Metagenomics of skin reveals insights into the human microbiome

...of the National Human Genome Research Institute have generated a diversity profile of human skin microbiota by sequencing 16S rRNA, a component of the prokaryotic ribosome, isolated from a specific region of skin. We focused this study on the inner elbow to inform future clinical studies of the extremely common...

New edition of a classic molecular biology textbook from CSHL Press and Benjamin Cummings

...by the new generation of genome technologies, and the elucidation of gene regulatory networks. The twenty-two chapters offer balanced coverage of prokaryotic and eukaryotic systems, and are divided into five major sections. The chapters in Part 1 provide a historical basis for the field and include descrip...

Symposium marks 30th anniversary of discovery of third domain of life

...ere not bacteria. Wolfe recalled, When Carl said they werent bacteria, I said: Of course they are bacteria! They look like bacteria! They have this prokaryotic morphology and cell structure. But when Wolfe saw how the sequence data fell into discrete groups, with all the methanogens in a category of their...

Metagenomics of the deep Mediterranean

...ing pure cultures, the power of high-throughput sequencing is applied directly to environmental samples to obtain information about the genomes of the prokaryotic cells present in a specific habitat studied. The ocean is an ideal subject of this approach because of its enormous microbiota, whose biomass equals t...

Metagenomics of the deep Mediterranean

...ing pure cultures, the power of high-throughput sequencing is applied directly to environmental samples to obtain information about the genomes of the prokaryotic cells present in a specific habitat studied. The ocean is an ideal subject of this approach because of its enormous microbiota, whose biomass equals t...

Researchers make nanosheets that mimic protein formation

...nto layers, called S-layers," Kotov said. S-layer proteins comprise the outermost cell envelope of a wide variety of bacteria and other single-celled, prokaryotic organisms called archaea, and they are able to form 2-d sheets with square, hexagonal, and other packings at surfaces and interfaces, as well as suspe...
Prokaryotic in Biological Technology

Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells

Mammalian Hamster fibroblast (CHO) Human B Human, B lymphomas: BJAB,P3HR-1, B95-8, UC729-6 Human, B-cell, JY, Epstein-Barr virus transformed Human, C-4I, cervical carcinoma Human epith...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

...ar biological techniques that enable amplification and sequencing of DNA extracted directly from the environment. The ubiquitous prokaryotic 16S ribosomal RNA gene has been the most widely targeted molecule for these studies because regions of nucleotide sequence conse...

Transformation of Filamentous Fungi by High-Voltage Electroporation

...mentous fungi. Recently, electroporation has become a valuable technique for transferring nucleic acids into both eukaryotic and prokaryotic cells (Miller et al., 1988; Fster and Neumann, 1989). In this report, we show the use of high voltage-mediated transformation as...

Biolistic Transfection of Organotypic Brain Slices

...ed, Biolistic bombardment has also been proven effective in transfecting a wide variety of animal tissues as well as in both eukaryotic and prokaryotic microbes, mitochondria, and microbial and plant chloroplasts (Johnston, 1990; Klein et al., 1992; Pecorino and Lo, 1992; Jiao et...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

...ers tested. After identification of the coding region, the corresponding fragment of the viral genome was cloned into a suitable prokaryotic expression vector and, following a careful optimization process, the recombinant protein was expressed in E. coli cells at a ver...

Which Water to Use?

...ade Water - Ambion's RT-PCR grade water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using ... end-point PCR assay based on detection of rRNA genes. prokaryotic DNA contamination is assessed by performing PCR with 1...

Translate More Protein with Higher Biological Activity

... Efficient synthesis of a wide range of prokaryotic and eukaryotic proteins ...ng most eukaryotic as well as prokaryotic proteins, often generating considerably higher yields. In prokaryotic transcription, the nascent 5'...

Translate High Yields of Active Protein

... Efficient synthesis of a wide range of prokaryotic and eukaryotic proteins Fast trans...gth, active protein from both prokaryotic and eukaryotic templates. ...ranslation system capable of expressing prokaryotic and eukaryotic sequences in 1-2 hours. ...

Increase Protein Activity and Yield

...ting kits have already illustrated ActivePro's superior ability to synthesize protein with higher biological activity from both prokaryotic and eukaryotic transcripts. Now, more recent data shows ActivePro's ability to synthesize higher yields of multiple proteins in the same re...

Bacterial RNA Isolation From Infected Eukaryotic Hosts

...m the total RNA through the seamless integration of Ambion's MICROB Express technology. This technology enables >95% removal of prokaryotic 16S and 23S rRNAs (Figure 2). See the article "Purify mRNA From Bacteria!" for information on MICROB Express . Figure...
Prokaryotic in Biological Products

Cloning and Expression Services from Molecular Biology Resources

Description:... Molecular Biological Resources has extensive abilities cloning and expression of prokaryotic and eukaryotic proteins. Please contact lloconne@molbiores.com to discuss your project. Contact lloconne@molbiores.com for more information....
Company:Molecular Biology Resources

Genes VI from Promega

Description:... Edited by Benjamin Lewin (1997) • This book gives an integrated account of the structure and function of genes in both prokaryotic organisms and is thoroughly up to date with the latest thinking and research in the field....
Company:Promega

TargeTron Gene Knockout System from Sigma-Aldrich

Description:...e PCR step to re-target the TargeTron group II intron for specific insertion into the host genome. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches. Compared to existing knockout methodologies, the TargeTron system is quite r...
Company:Sigma-Aldrich

loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

Description:... The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modif...
Company:Gene Bridges GmbH

FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH

Description:... The FRT-amp-FRT cassette is designed to allow ampicillin selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modif...
Company:Gene Bridges GmbH

* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH

Description:...gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eu...
Company:Gene Bridges GmbH

loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

Description:...e loxP-cm-loxP cassette is designed to allow chloramphenicol selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for chloramphenicol resistance is a slightly ...
Company:Gene Bridges GmbH

* Quick & Easy Conditional Knock Out Kit - loxP from Gene Bridges GmbH

Description:...b2-neo-loxP cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eu...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH

Description:... designed to allow kanamycin/neomycin selection in prokaryotic and eukaryotic cells, respectively. It combines a prokaryotic promoter (gb2) for expression of kanamycin resista...on of neomycin resistance in mammalian cells. The prokaryotic promoter gb2 is a slightly modified version of the...
Company:Gene Bridges GmbH

loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH

Description:... designed to allow neomycin/kanamycin selection in prokaryotic and eukaryotic cells. The loxP-PGK-gb2-neo-loxP t...eomycin/kanamycin resistance gene which combines a prokaryotic promoter (gb2) for expression of kanamycin resista...on of neomycin resistance in mammalian cells. The prokaryotic promoter gb2 is a slightly modified version of the...
Company:Gene Bridges GmbH
Prokaryotic in Biological Definition

Alga

... 1 Relationships among algal groups 1.1 prokaryotic algae 1.2 Eukaryotic algae 2 Forms of a... Relationships among algal groups prokaryotic algae Traditionally the cyanobacteria have be...reating Earth's oxygen atmosphere. They have a prokaryotic cell structure typical of bacteria and conduct ph...

Archaea

...d by rate-invariant analysis of rRNA sequences. Nature 331 184–186. Woese, Carl R.; Fox, George E. (1977). Phylogenetic Structure of the prokaryotic Domain: The Primary Kingdoms. Proceedings of the National Academy of Sciences of the United States of America 74 (11) 5088–5090. ...

Cell division

...is leads to growth in multicellular organisms (the growth of tissue ) and to procreation ( vegetative reproduction ) in unicellular organisms . prokaryotic cells divide by binary fission . Eukaryotic cells usually undergo a process of nuclear division, called mitosis , followed by division of the ce...

Cellular respiration

...such losses as the cost of moving pyruvate into mitochondria. This takes place in the mitochondria in eukaryotic cells , and at the cell membrane in prokaryotic cells . Anaerobic respiration (Fermentation) " Anaerobic respiration" It does not require oxygen. True anaerobic respiration involves an elec...

Cytoskeleton

...ediate Filaments 1.3 Microtubules 2 The prokaryotic cytoskeleton 2.1 FtsZ 2.2 MreB and ParM ... synthesis of the cell wall in plants. The prokaryotic cytoskeleton The cytoskeleton was previously con... provides strong evidence that the eukaryotic and prokaryotic cytoskeletons are truly homologous. FtsZ F...

Endoplasmic reticulum

... is an organelle found in all eukaryotic cells . The ER modifies proteins , makes macromolecules , and transfers substances throughout the cell. prokaryotic organisms do not have organelles and thus do not have an ER. ER's base structure and composition is similar to the plasma membrane , though it is a...

Endosymbiotic theory

...les of eukaryotic cells. According to this theory, these originated as prokaryotic endosymbionts , which came to live inside eukaryotic cells. The theory p...pared to the other membranes in the cell. The composition is like that of a prokaryotic cell membrane. New mitochondria and chloroplasts are formed only through...

Enzyme

...to glucose Dihydrofolate reductase : reduces dihydrofolic acid to tetrahydrofolic acid DNA gyrase : catalyzes the negative supercoiling of prokaryotic DNA Glutamate decarboxylase : synthesizes the neurotransmitter GABA in the brain Glutathione peroxidase : protects cells by converting hydrog...

Eukaryote

...called flagella when long and cilia when short, that are variously involved in movement, feeding, and sensation. These are entirely distinct from prokaryotic flagella. They are supported by a bundle of microtubules arising from a basal body, also called a kinetosome or centriole, characteristically arrange...

Flagellum

...archaeal flagella have only recently begun to get serious scientific attention. Therefore many assume erroneously that there is only one basic kind of prokaryotic flagellum, and that archaeal flagella are homologous to it (an example is Cavalier-Smith (2002), who is aware of the differences in archaeal and bacte...
Prokaryotic in Biological Dictionary

Wobble base pair

... base pair - Wikipedia, the free encyclopedia ... " Wobble " base pairing and code degeneracy. Ribosome Structure. TRANSLATION PROCESS - prokaryotic ... recognition and " wobble " base pairing . UH2. UH2. I ... Base Pair Type: GU wobble . Base Pair Category: ... G-U, Wobble . 3' 5' S...

Unicellular

... ... while processing this directive] Types of unicellular organisms ... unicellular . organisms , all of which fall into two general categories: prokaryotic ... They are unicellular organisms and lack organelles, including a nucleus. ... Unicellular eukaryotes are those whose members consist of a ...

Start codon

... There is often more than one AUG codon in an mRNA, and the small ribosomal ... start codon prediction is the most difficult remaining part of prokaryotic ... Among the possible start codon candidates, what extra evidence ... start codon ... How do 64 different codons produce 20 different am...

Sigma Factor

... A sigma factor (σ factor ) is a prokaryotic transcription initiation factor that enables specific binding of RNA polymerase to gene promoters. Different sigma factors are activated in ...

Ribosomes

...unction at the Department of Chemistry and ... Ribosomes occur in both prokaryotic and eukaryotic cells. ... Interestingly, the ribosomes in the mitochondr...ogy-Online.org dictionary. ... Ribosomes are formed in the cytoplasm of prokaryotic cells. ... Brief and Straightforward Guide: What is a Ribosome ? ... It...

Ribosome

...formation about ribosome in the free online English dictionary and encyclopedia. ... ribosome (rb-sm) ... Ribosomes exist in both eukaryotic and prokaryotic cells. ... Ribosome summary with 19 pages of encyclopedia entries, essays, summaries, research information, and more. ... Global Motions and Corr...

Ribosomal RNA

...m for decoding mRNA into amino acids and to interact... The 16S rRNA is a part of the ribosomal RNA −-- a 1542 nt long component of the small prokaryotic ribosomal subunit (30S). Ribosomal RNA (rRNA) is a component of the ribosomes, the protein synthetic ... They make up at least 80% of the ...

Recombinant clone

...mation >> Click ... There are totally 15 chapters at this laboratory manual. ... Recombinant DNA cloning vectors and the eukaryotic and prokaryotic ... ...

Pseudogene

...r. Scientific ... VIEW PSEUDOGENE FAMILIES starting from PROTEIN FAMILIES. Human (Homo sapiens) ... Pseudogene assignments for an assortment of prokaryotic genomes are available here. ... pseudogene ( ) n. A defective segment of DNA that resembles a gene but cannot be transcribed. ... A pseudoge...

Prophage

.... Prophage loci often remain under-annotated or even unrecognized in prokaryotic ... Prophage Finder was tested using previously annotated prokaryotic genomic ... ...
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