Dermaseptins: Potent vaginal contraceptives
...ts can be used vaginally in effective doses without causing overt vaginal irritation or other toxicity. Antimicrobial peptides are produced by both prokaryotic and eukaryotic cells and have been the subject of many studies in recent years because of their involvement in host defense mechanisms and their poten...JCVI program trains USDA scientists on eukaryotic genome analysis
... a deeper understanding of the annotations available in publicly accessible databases. In addition to the training, JCVI offers free annotation of prokaryotic genomes through the JCVI Annotation Service. This service has provided more than 100 investigators with more than 200 genomic sequences. Accordin...Research-based undergraduate course expands beyond Washington University
...ing on the challenge of working with eukaryotic genomes. These genomes are more complex and orders of magnitude larger than the more commonly studied prokaryotic (for example, bacterial) genomes. The development of a coordinated research effort that pools student contributions across institutions allows the GE...Researchers develop new self-training gene prediction program for fungi
...the first completely sequenced genomes of bacteria and archea. The research team then developed self-training versions of the gene finding program for prokaryotic (organisms that lack a cell nucleus) and eukaryotic (organisms that contain a cell nucleus) genomes in 2001 and 2005, respectively. Development of the...Metagenomics of skin reveals insights into the human microbiome
...of the National Human Genome Research Institute have generated a diversity profile of human skin microbiota by sequencing 16S rRNA, a component of the prokaryotic ribosome, isolated from a specific region of skin. We focused this study on the inner elbow to inform future clinical studies of the extremely common...New edition of a classic molecular biology textbook from CSHL Press and Benjamin Cummings
...by the new generation of genome technologies, and the elucidation of gene regulatory networks. The twenty-two chapters offer balanced coverage of prokaryotic and eukaryotic systems, and are divided into five major sections. The chapters in Part 1 provide a historical basis for the field and include descrip...Symposium marks 30th anniversary of discovery of third domain of life
...ere not bacteria. Wolfe recalled, When Carl said they werent bacteria, I said: Of course they are bacteria! They look like bacteria! They have this prokaryotic morphology and cell structure. But when Wolfe saw how the sequence data fell into discrete groups, with all the methanogens in a category of their...Metagenomics of the deep Mediterranean
...ing pure cultures, the power of high-throughput sequencing is applied directly to environmental samples to obtain information about the genomes of the prokaryotic cells present in a specific habitat studied. The ocean is an ideal subject of this approach because of its enormous microbiota, whose biomass equals t...Metagenomics of the deep Mediterranean
...ing pure cultures, the power of high-throughput sequencing is applied directly to environmental samples to obtain information about the genomes of the prokaryotic cells present in a specific habitat studied. The ocean is an ideal subject of this approach because of its enormous microbiota, whose biomass equals t...Researchers make nanosheets that mimic protein formation
...nto layers, called S-layers," Kotov said. S-layer proteins comprise the outermost cell envelope of a wide variety of bacteria and other single-celled, prokaryotic organisms called archaea, and they are able to form 2-d sheets with square, hexagonal, and other packings at surfaces and interfaces, as well as suspe...Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells
Mammalian Hamster fibroblast (CHO) Human B Human, B lymphomas: BJAB,P3HR-1, B95-8, UC729-6 Human, B-cell, JY, Epstein-Barr virus transformed Human, C-4I, cervical carcinoma Human epith...Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
...ar biological techniques that enable amplification and sequencing of DNA extracted directly from the environment. The ubiquitous prokaryotic 16S ribosomal RNA gene has been the most widely targeted molecule for these studies because regions of nucleotide sequence conse...Transformation of Filamentous Fungi by High-Voltage Electroporation
...mentous fungi. Recently, electroporation has become a valuable technique for transferring nucleic acids into both eukaryotic and prokaryotic cells (Miller et al., 1988; Fster and Neumann, 1989). In this report, we show the use of high voltage-mediated transformation as...Biolistic Transfection of Organotypic Brain Slices
...ed, Biolistic bombardment has also been proven effective in transfecting a wide variety of animal tissues as well as in both eukaryotic and prokaryotic microbes, mitochondria, and microbial and plant chloroplasts (Johnston, 1990; Klein et al., 1992; Pecorino and Lo, 1992; Jiao et...Translate More Protein with Higher Biological Activity
... Efficient synthesis of a wide range of prokaryotic and eukaryotic proteins ...ng most eukaryotic as well as prokaryotic proteins, often generating considerably higher yields. In prokaryotic transcription, the nascent 5'...Translate High Yields of Active Protein
... Efficient synthesis of a wide range of prokaryotic and eukaryotic proteins Fast trans...gth, active protein from both prokaryotic and eukaryotic templates. ...ranslation system capable of expressing prokaryotic and eukaryotic sequences in 1-2 hours. ...Increase Protein Activity and Yield
...ting kits have already illustrated ActivePro's superior ability to synthesize protein with higher biological activity from both prokaryotic and eukaryotic transcripts. Now, more recent data shows ActivePro's ability to synthesize higher yields of multiple proteins in the same re...Bacterial RNA Isolation From Infected Eukaryotic Hosts
...m the total RNA through the seamless integration of Ambion's MICROB Express technology. This technology enables >95% removal of prokaryotic 16S and 23S rRNAs (Figure 2). See the article "Purify mRNA From Bacteria!" for information on MICROB Express . Figure...Cloning and Expression Services from Molecular Biology Resources
Description:... Molecular Biological Resources has extensive abilities cloning and expression of prokaryotic and eukaryotic proteins. Please contact lloconne@molbiores.com to discuss your project. Contact lloconne@molbiores.com for more information....TargeTron Gene Knockout System from Sigma-Aldrich
Description:...e PCR step to re-target the TargeTron group II intron for specific insertion into the host genome. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches. Compared to existing knockout methodologies, the TargeTron system is quite r...loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH
Description:... The loxP-amp-loxP cassette is designed to allow ampicillin selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modif...FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
Description:... The FRT-amp-FRT cassette is designed to allow ampicillin selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modif...* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH
Description:...gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eu...loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH
Description:...e loxP-cm-loxP cassette is designed to allow chloramphenicol selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for chloramphenicol resistance is a slightly ...* Quick & Easy Conditional Knock Out Kit - loxP from Gene Bridges GmbH
Description:...b2-neo-loxP cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eu...