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Primers in Medical News

TriLink BioTechnologies, Inc. Announces Sales and License Agreement With Glen Research Corporation for CleanAmp(TM) Amidites

... the CleanAmp(TM) Amidites available through Glen Research allows institutions with oligonucleotide synthesis capabilities to manufacture CleanAmp(TM) primers in-house. Learn more at www.trilinkbiotech.com/cleanamp . "Glen Research is dedicated to bringing the very best research products to the olig...

New accurate diagnostic test for swine H1N1 influenza using RT-PCR technology

...etic material, enabling rapid detection of new viral strains as they evolve. Using these genetic sequence data and making minor alterations to the PCR primers used in the assay, the test could be easily modified to detect newly emerging viral variants, including avian influenza strains. "Early recognitio...

Booster vaccination may help with possible future avian influenza pandemic

...ccompanying editorial, Gregory A. Poland, MD, of the Mayo Clinic College of Medicine, noted that some are already looking to begin such prevaccination primers against H5N1 influenza. For example, Japan is planning to immunize health care workers starting in 2009, and the U.S. Department of Defense is offerin...

Recently discovered virus associated with pediatric respiratory tract infection in Germany

...e genomes of the target viruses or bacteria. If a throat swab contains pathogens with nucleic acid sequences that match those of the primers, then the primers will copy the target DNA several million times. Likewise the molecular tags, different in mass for each of the primers, are also amplified making them...

Lab-on-chip testing for 'bird flu' developed by Singapore scientists

...oping the H5N1 detection kits and thus accumulated technical knowledge like primers and cycling conditions for the PCR assay. We are very pleased to see that t...cid diagnostic primers, played a role in the initial designs of the PCR and primers assays in this project. "We have a long journey ahead of us in our batt...
Primers in Medical Technology

TriLink Launches CleanAmp(TM) dNTPs for Hot Start PCR

... CEO and President of TriLink. "TriLink now offers two unique hot start PCR solutions: CleanAmp(TM) dNTPs and CleanAmp(TM) Primers. The CleanAmp(TM) primers will solve specific assay issues whereas CleanAmp(TM) dNTPs will be a more general solution. Either product will provide a very cost effective soluti...

TriLink Awarded a Phase II Grant for a Second Hot Start PCR Project

...d Dr. Richard Hogrefe, CEO and President of TriLink. "Now we offer modified primers and modified dNTPs. The CleanAmp(TM) primers will solve specific assay issues whereas the modified dNTPs will be a more ...

The Past, Present and Future of HLA Typing

...isen; for example, it is possible to construct PCR primers pre labeled with biotin, and then amplify biotin ...y to detect HLA polymorphisms. In this technique, primers can be constructed specifically to complement HLA polymorphisms; if the primers bind the complementary polymorphism and amplify th...
Primers in Medical Products

Flu A/B ASR

Description:... The Flu A/B ASR contains primers and a FAM-labeled probe that is designed to detect Flu A and an Alexa 532-labeled probe to detect Flu B. In addition, this ASR contains primers and a Texas Redlabeled probe for a separate sample processing control. This...
Company:Cepheid

mecA ASR

Description:... The mecA ASR contains primers and a FAM-labeled probe that is designed to detect a 146 bp region of the mecA gene. In addition, this ASR contains primers, a Texas Red-labeled probe...
Company:Cepheid

Mycoplasma pneumoniae ASR

Description:... The Mycoplasma pneumoniae ASR contains primers and a FAM-labeled probe that is designed to detect the P1 adhesion gene. In addition, this ASR contains primers, a Texas Red-labeled probe and DNA f...
Company:Cepheid

RSV ASR

Description:... The RSV ASR contains primers and a FAM-labeled probe that is designed to detect Respiratory Syncytial Virus (RSV). In addition, this ASR contains primers and a Texas Red-labeled probe for a separate sample processing control. T...
Company:Cepheid

Staphylococcus aureus (SPA) ASR

Description:... The Spa ASR contains primers and a FAM-labeled probe that is designed to detect a 98 bp region of the Staphylococcus protein A gene. In addition, this ASR contains primers, a Texa...
Company:Cepheid

Certified Low Range Ultra Agarose

Description:... This agarose provides superior resolution of small PCR fragments and primers at lower concentrations than standard agaroses. A 3% gel clearly resolves a 10 bp ladder, and a 4% gel approaches the resolution of an 8% polyacrylami...
Company:Bio-Rad Lab Equipment

Dynal RELI™ SSO

Description:... The Dynal RELI™ SSO typing technique utilises a generic PCR amplification with biotinylated primers that flank the specific target region of an HLA locus. This is followed by hybridisation of the resulting amplicon to an array of Sequence Specific Ol...
Company:Dynal Biotech, LLC

HLA SSP Trays

Description:...mbinations in the PCR amplification. It is based on the principle that only primers whose sequence is completely complementary to a target sequence will be converted by the polymerase into PCR products. In contrast, non-complementary primers produce no amplification product. Using the Biotest SSP Kit Systems, reliab...
Company:Biotest

HLA SSP Kits

Description:...mbinations in the PCR amplification. It is based on the principle that only primers whose sequence is completely complementary to a target sequence will be converted by the polymerase into PCR products. In contrast, non-complementary primers produce no amplification product. Using the Biotest SSP Kit Systems, reliab...
Company:Biotest

Parvovirus (Erythrovirus) B-19 ASR

Description:... The Parvo B19 ASR contains primers and a FAM-labeled probe targeting the VP1 gene of Parvovirus B19. In addition, the Parvo B19 ASR contains primers and a Texas Red-labeled probe for an internal control sequence. This ASR re...
Company:Cepheid
Primers in Biological News

Interdisciplinary volume on biological rhythms serves as both primer and in-depth resource

...n of Clocks and Rhythms provides an overview of the field of biological rhythms, providing an introduction to the field of chronobiology, as well as primers on the molecular basis of biological rhythms, pacemakers, and sleep. Subsequent sections of the book offer in-depth discussions on specific topics, i...

Priming scientists for successful media interviews

...er for seasoned expertsan ideal text for a graduate course on science communication or a professional development course for students and faculty. The primers authors speak from their own experiences as PhD scientists in the biological sciences with years of experience in media outreach. The concise, user...

ATR checkpoint-activating DNA structure

... responses in unprecedented ways." Using Xenopus egg extracts as a cell-free system, the researchers found that single-stranded DNA annealed with primers specifically triggers the ATR-mediated checkpoint response. While many proteins involved in the DNA damage response have been uncovered, this type of ...
Primers in Biological Technology

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient Eng-Lai Tan and Choon-Kook Sam NPC Laboratory, Institute of Postgraduate Studies and Research, University of...

New PCR Primers for Human Diseases

Screen samples for disease states or pathogens Gabriela M. Tobal Stratagene Cloning Systems, Inc. W. S. Nichols Stratagene is introducing PCR primer sets for detecting 13 human diseases. The sequences of these primers have been published previously and are well character...

RT-PCR Primers for the Study of Apoptosis

Investigating programmed cell death Wei-ping Yang Danny Hoang Douglas McKenzie Peter Pingerelli Stratagene The apoptotic response is a complex biological process that requires the regulation of a variety of genes that interact with and respond to intracellular and extr...

Gene-Specific Primers for NMDA Receptor Subunits

Additional tools for neuroscience researchers Robert Buchner Peter L. Pingerelli Michael D. Browning Mary Ellen Simcox Stratagene Cloning Systems, Inc. Stratagene is now offering gene-specific primer sets for the rat and human NMDA receptor. In this article, we descr...

Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance

Characterize gene distribution in your cDNA Brenda Rogers Douglas McKenzie Stratagene We introduce six pairs of control RT-PCR primers that are designed to detect specific human gene transcripts with various expression levels. These primer sets provide insig...

Choice of reverse transcription primers

Priming affects the size and specificity of the cDNA produced. There are three types of primers that may be used for reverse transcription: Oligo(dT)1218, which binds to the endogenous poly(A) + tail at...

Jivan Focuses on Solutions for Transcriptome Sequencing

...owers scientists with new insight by providing a full range of products and services for mRNA sequencing and expression, including capture arrays, PCR primers and amplicons, and transcriptome sequencing software. Contact: Jonathan Bingham jbingham@jivanbio.com +1 415 425 4115 ...

GeneCopoeia Introduces Genome-Wide MicroRNA (miRNA) Functional Analysis Tools

...ystem in the next few weeks. This system consists of a reverse transcription kit, a SYBR green RT-qPCR master mix kit, and a complete set of validated primers for human, mouse, and rat miRNAs, which allows specific and sensitive quantification of miRNAs individually or in an array format. GeneCopoeia al...

Data on WaferGen's SmartChip(TM) System Presented at 16th International Molecular Medicine Tri-Conference

...ds. Successful demonstration of key SmartChip system workflow elements, including primer-ready SmartChips and the drying and reconstitution of primers for rapid high-throughput 100 nL real-time PCR. It is important to note that all of these results were achieved using clinical biolo...

WaferGen's SmartChip(TM) System Performance Successfully Demonstrated in Initial Phase of Alpha Testing Program

...y patients. Key workflow elements that have been successfully demonstrated include primer-ready SmartChips and the drying and reconstitution of primers for rapid high-throughput 100 nL real-time polymerase chain reaction (PCR). Additionally, the platform's ability to perform PCR in nano-volumes withou...

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Primers in Biological Products

EcoRI-ACA AFLP Amplification Primers from Applied Biosystems

Description: ABI Plant AFLP Kits • EcoRI-ACA AFLP Amplification Primers...
Company:Applied Biosystems

Custom DNA Primers from Invitrogen

Description: ...
Company:Invitrogen

MasterAmp Taq PCR Core Kit from EPICENTRE Biotechnologies

Description:...Taq DNA Polymerase, 10X PCR Buffer, MasterAmp™ 10X PCR Enhancer, dNTP Mix, 2.5 mM each, 25 mM MgCl2, Enzyme Dilution Buffer, Control Template and primers Mix...
Company:EPICENTRE Biotechnologies

PCR-CleanUp Mix from Yorkshire Bioscience Ltd

Description:... All-in-one mix, removes primers and dNTPs from PCR product for following direct sequencing...
Company:Yorkshire Bioscience Ltd

mirVana™ qRT-PCR Primer Set for Normalization (5S) from Ambion

Description:... 53 new primer sets added in Dec 2005 Quantitative results in less than 3 hours Detection from as little as 25 pg of total RNA Specific primers available for hundreds of mammalian miRNAs Suitable for either real-time or end-point RT-PCR Ideal for validating expression profiling data an...
Company:Ambion

Human TFR Template & Primer Set from Takara Mirus Bio

Description:... Template and primers for preparation of internal control spots...
Company:Takara Mirus Bio

Lambda Control Template and Primer from Takara Mirus Bio

Description:... Template and primers for preparation of internal control spots...
Company:Takara Mirus Bio

Oligo Synthesis from Biosearch Technologies

Description:...iding advanced oligonucleotide tools to accelerate the discovery and application of genomic information. Whether you need dual-labeled FRET probes and primers or 100 or 100,000 custom oligos for microarray applications, Biosearch can meet your most stringent and exacting synthesis, purification, QA/QC and do...
Company:Biosearch Technologies

RNAimage Kit from GenHunter

Description:... For mRNA Differential Display with three one-base anchored oligo-dT primers and rationally designed arbitrary 13mers. These kits feature the latest generation of isotopic mRNA Differential Display technology....
Company:GenHunter

Panomer 9 random oligodeoxynucleotide, Alexa Fluor 647 conjugate from Molecular Probes (Invitrogen)

Description:... Panomer 9 oligodeoxynucleotides are random primers that are labeled at the 5' end with our proprietary Alexa Fluor dyes and they are useful for assessing microarray spot morphology, hybridization poten...
Company:Molecular Probes (Invitrogen)
Primers in Biological Definition

Polymerase chain reaction

... 2 Patent wars 3 PCR in practice 3.1 primers 3.2 Procedure 3.3 Example 3.4 PCR optimiz...egion of the DNA fragment to be amplified Two primers , which determine the beginning and end of the re...cost in the order of USD 2,500 in 2004 . primers The DNA fragment to be amplified is determine...

Primer

...merase chain reaction (PCR), require primers. The primers used for these techniques are usually short (about...ed DNA molecules. The actual construction of such primers starts with 3'-hydroxyl nucleosides attached to a ...ed. For that reason, most laboratories do not make primers themselves, but order them by specialized companie...
Primers in Biological Dictionary

Taq polymerase

...erase . Anti- Taq Monoclonal ... Generally, standard Taq DNA Polymerase has difficulty amplifying targets >5 kb. ... general guidelines for primers to be used with Takara's Ex Taq ™ Polymerase ? ... Britannica online encyclopedia article on taq polymerase (enzyme), ...replenished after ev...

Simple Sequence Repeat

..... repeat , and repeated sequence on the title line with the sequence following. ... PCR analysis using anchored simple . sequence repeat primers has gained attention recently ... inheritance of inter- simple sequence repeat polymorphisms ... Comparison of the genomes of mouse and ...

Sequence tagged site

...equence - tagged sites (Microsatellites, SCARs, CAPS, ISSRs) DNA-based ... Unlike PCR with arbitrary primers, sequence - tagged sites (STS) are primers that are ... Sequence - tagged site - Wikipedia, the free encyclopedia ... Sequence - Tagged Sites (STS) Sequence - Tagged Sites (STS),...

Saturation mutagenesis

...tagenesis is ... Perform saturation mutagenesis . Eliminates difficult restriction cloning ... Saturation Mutagenesis . Primer Strategy: primers With One or More Degenerate Codons ... Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes. Iterative ...

RFLP

...3% NuSieve (Cambrex) agarose gel for RFLP . ... Perform PCR with Beckman D4-labeled (5'-end) forward 18S rDNA primer and ... Custom D4-labeled primers are ordered from Proligo.com. ... posted to therapeutic rflp recombinant mtdna mouse mitotarget mitochondria ... posted to rflp snp by ...

Primase

... Copy] ... posted to atr primase rad17 rfc rpa by DNAReplication on 2008-01-02 18:45:20 as ... The European ... DNA primase synthesizes the RNA primers for the Okazaki ... In Escherichia coli, primase (DnaG) interacts with the replicative ... The European ... similarity to the central region of...

Polymorphic loci

... Comparison of agarose type (non- polymorphic loci ) ... These primers amplify polymorphic loci , and alleles of different sizes can be observed. ... Many polymorphic loci have been identified, and scientists ...

Overlapping clones

...ed in an overlapping series that ... of 7,700 clusters of overlapping clones - or "contigs" - that combine together ... PCR analysis with STS primers was used to screen for overlapping clones and ... in screening for overlapping BAC clones by generating many bands which are specific ... ...

Nonsense Mutation

...o ultraviolet or... FBN1 mRNAs carrying the Y2113X nonsense mutation are subject to NMD. ... The Y2113X nonsense mutation is indicated, the primers used in the RT–PCR assay ... BioInfoBank Library :: beta 0 thalassemia, a nonsense mutation in man. Coding capacity of complementary DNA strand...

Minisatellite

...ncy of these mutants in sperm. ... Directed Amplification of Minisatellite DNA ... A number of minisatellite core sequences were used as single primers to amplify genomic DNA. ... Potential Forensic Applications of Minisatellite Variant Repeat (MVR) Mapping ... Minisatellite variant repeat (MVR...
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