Stretching DNA to the limit
...u is drastically reduced in width with increasing exposure to UV light.
UV light induces the crosslinking of the constituent DNA bases within the polynucleotide
chains, as well as causes the formation of lesions by linking together the adjacent strands. The small changes in structure induced by this crosslinki...
Polynucleotide in Biological Technology
Evaluation of FlashPlate in a Helicase Assay
...M NaCl, 1 mM DTT, 0.5 mM MnC l 2 , 20% glycerol and 1 mM ATP)
Kinase (T4) (NEE101)
[ 33 P]-γ-ATP (NEG602H)
1. DNA oligo #2 was 5'-labeled with 33 P by T4 polynucleotide
kinase, following the manufacturer's protocol.
2. Equal molar qu...
USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends
Wild-type T4 polynucleotide
Kinase (PNK) (1,2,3) is commonly used for phosphorylation and labeling of 5'-ends of DNA and oligonucleotides. Labeling, in particular, can be accomp...
The Bio-Rad Silver Stain, Rev E
...ptic vesicles, Proc Natl Acad Sci USA 82, 41374141 (1985)
33. Khan ZA and Fraenkel-Conrat H, Purification and characterization
phosphorylase from cucumber, Proc Natl Acad Sci USA
82, 13111315 (1985)
34. King SM et al., Characterization of monoclonal antibo...
Rapidly Prepare Probes and Markers for Small RNA Analysis
...Markers Preparation of RNA
markers and DNA or RNA probes starts with a phosphorylation
reaction using T4 polynucleotide
Kinase and Kinase Buffer
supplied with the kit, and [gamma- 32 P]ATP. If
required, Kinase Buffer, enzyme, an...
Highly Sensitive microRNA Array Performance
...d oligonucleotides with sequences
complementary to the known human, mouse, and rat miRNAs. The
probes have a polynucleotide
spacer that ensures maximal
hybridization efficiency to labeled miRNAs when the probes are
arrayed on amine-...
Human Universal cDNA Library Array I
...idization were performed in 6X SSC buffer, 5X
Denhardts reagent, and 0.1% SDS. The oligonucleotide probes were
prepared using T4 polynucleotide
kinase and high-specific-activity [g- 32 P]ATP
(3000 Ci/mmol). The specific activity for the probes were approximately
5 10 8 ...
Polynucleotide in Biological Products
T4 Polynucleotide Kinase Exchange Reaction Buffer from Invitrogen
Description: For use with T4 Polynucleotide
Kinase for labeling of 5' ends (5' phosphate ends) of nucleic acids. In the exchange reaction T4 Polynucleotide
Kinase transfers the 5' phosphate group from DNA to ADP and subsequently transfers the Gamma-phosphate of ATP to the free 5' hydroxyl group to rephosphoryla...
T4 Polynucleotide Kinase from Promega
Description: T4 Polynucleotide
Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5-terminus of polynucleotides or to mononucleotides bearing a 5-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent ...
T4 Polynucleotide Kinase from EPICENTRE Biotechnologies
Description: T4 Polynucleotide
Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removes the 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside 3'-monophosphates, and...
Calf Alkaline Phosphatase, Calf Intestinal, Unconjugated from Promega
Description:...from both 5-termini and may also be used for the dephosphorylation of 5 phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 polynucleotide
Kinase. CIAP is active on 5 overhangs, 5 recessed and blunt ends (15)....
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Description:...ning DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide
with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates for...
RNA Polymers - Poly rA. 2 Poly rU Triple-Stranded Polymer, sodium salt from Midland Certified Reagent Company
Description:... Polyribonucleotides (RNA polymers) are prepared by the catalytic action of the enzyme, polynucleotide
phosphorylase, isolated at Midland, and the appropriate, ribonucleoside diphosphate as substrate. With the exception of catalog number P-3102, RNA pol...
Company:Midland Certified Reagent Company
Polynucleotide in Biological Definition
... referred to by the identity of their bases. These are adenine (A), thymine (T), cytosine (C), and guanine (G).
In a DNA double helix, two polynucleotide
strands can associate through the hydrophobic effect . Specificity of which strands stay associated is determined by complementary pairing . Each ba...
Polynucleotide in Biological Dictionary
... In enzymology, a polynucleotide
5'-hydroxyl- kinase (EC 126.96.36.199) is an enz... that catalyzes the chemical reaction Use this polynucleotide
kinase to: phosphorylate 5_ -ends of DNA or RNA ... T4 polynucleotide
Kinase ; 3'-phosphatase-free. Alkaline Phosphata...
...NA polymerase get its energy to make phosphodiester bond ? ... phosphodiester bond n. The covalent chemical bond that holds together the polynucleotide
chains of RNA and DNA by joining a carbon in the pentose The phosphate groups in the phosphodiester bond are negatively-charged. ... A phosphod...
... singles, watch music ... Okazaki Fragments's Latest Blog Entry [Subscribe to this Blog] ... Details on Okazaki Fragments , DNA Polymerases, polynucleotide
Ligases, Okazaki , Vitro Synthesis, Replication of DNA ... that Okazaki fragments are initiated ... The initiator RNAs of mammalian Okazaki ...
...arge and important, but it is only one family in a ... Hence, the protein kinase -like superfamily has undergone substantial structural ... T4 polynucleotide
Kinase catalyzes the transfer and exchange of P<sub>i</sub> from the γ position of ATP to the 5' -hydroxyl terminus of double- and singl...
... Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide
chain. A hydrolyzing reaction occurs that breaks phosphodiester bonds at either the 3’ or 5’ ends. Its close relative... exonuclease activity....
...hod utilises a concatemer of the target polynucleotide, i.e. repeating ... The concatemer may be formed in any suitable way. ... ... each polynucleotide
comprises a concatemer having multiple copies of a target ... distributed on the surface in a manner to promote the fixing of one concatemer ... ...
.... What is codon ? Meaning of codon medical term. What does codon mean? ... codon /co·don/ (ko'don) a series of three adjacent bases in one polynucleotide
... Codon . National Human Genome Research Institute (NHGRI) by artist Darryl Leja. Look up a description of Codon in the NHGRI Talking Glossary...
...ucture of the sequenced molecule. base sequence ( ′bās ′sēkwəns ) ( genetics ) The specific order of purine and pyrimidine bases in a polynucleotide
such as deoxyribonucleic acid or sequence ( ) n. A following of one thing after another; succession. ... dna having the sequence a-g-g-c-g-...