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Polynucleotide in Biological News

Stretching DNA to the limit

...u is drastically reduced in width with increasing exposure to UV light. UV light induces the crosslinking of the constituent DNA bases within the polynucleotide chains, as well as causes the formation of lesions by linking together the adjacent strands. The small changes in structure induced by this crosslinki...
Polynucleotide in Biological Technology

Evaluation of FlashPlate in a Helicase Assay

...M NaCl, 1 mM DTT, 0.5 mM MnC l 2 , 20% glycerol and 1 mM ATP) polynucleotide Kinase (T4) (NEE101) [ 33 P]-γ-ATP (NEG602H) Pr...edure 1. DNA oligo #2 was 5'-labeled with 33 P by T4 polynucleotide kinase, following the manufacturer's protocol. 2. Equal molar qu...

USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5'-Ends

... Wild-type T4 polynucleotide Kinase (PNK) (1,2,3) is commonly used for phosphorylation and labeling of 5'-ends of DNA and oligonucleotides. Labeling, in particular, can be accomp...

The Bio-Rad Silver Stain, Rev E

...ptic vesicles, Proc Natl Acad Sci USA 82, 41374141 (1985) 33. Khan ZA and Fraenkel-Conrat H, Purification and characterization of polynucleotide phosphorylase from cucumber, Proc Natl Acad Sci USA 82, 13111315 (1985) 34. King SM et al., Characterization of monoclonal antibo...

Rapidly Prepare Probes and Markers for Small RNA Analysis

...Markers Preparation of RNA markers and DNA or RNA probes starts with a phosphorylation reaction using T4 polynucleotide Kinase and Kinase Buffer supplied with the kit, and [gamma- 32 P]ATP. If required, Kinase Buffer, enzyme, an...

Highly Sensitive microRNA Array Performance

...d oligonucleotides with sequences complementary to the known human, mouse, and rat miRNAs. The probes have a polynucleotide spacer that ensures maximal hybridization efficiency to labeled miRNAs when the probes are arrayed on amine-...

Human Universal cDNA Library Array I

...idization were performed in 6X SSC buffer, 5X Denhardts reagent, and 0.1% SDS. The oligonucleotide probes were prepared using T4 polynucleotide kinase and high-specific-activity [g- 32 P]ATP (3000 Ci/mmol). The specific activity for the probes were approximately 5 10 8 ...
Polynucleotide in Biological Products

T4 Polynucleotide Kinase Exchange Reaction Buffer from Invitrogen

Description: For use with T4 Polynucleotide Kinase for labeling of 5' ends (5' phosphate ends) of nucleic acids. In the exchange reaction T4 Polynucleotide Kinase transfers the 5' phosphate group from DNA to ADP and subsequently transfers the Gamma-phosphate of ATP to the free 5' hydroxyl group to rephosphoryla...
Company:Invitrogen

T4 Polynucleotide Kinase from Promega

Description: T4 Polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5-terminus of polynucleotides or to mononucleotides bearing a 5-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent ...
Company:Promega

T4 Polynucleotide Kinase from EPICENTRE Biotechnologies

Description: T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-hydroxyl of single- and double-stranded DNA, RNA, and nucleoside 3'-monophosphates. The enzyme also removes the 3'-phosphate from 3'-phosphoryl polynucleotides, deoxyribonucleoside 3'-monophosphates, and...
Company:EPICENTRE Biotechnologies

Calf Alkaline Phosphatase, Calf Intestinal, Unconjugated from Promega

Description:...from both 5-termini and may also be used for the dephosphorylation of 5 phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 polynucleotide Kinase. CIAP is active on 5 overhangs, 5 recessed and blunt ends (15)....
Company:Promega

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:...ning DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with heat. Although UNG is active on single- and double-stranded dU-containing DNA,2 ribouracil residues in RNA and dUTP itself are not substrates for...
Company:Applied Biosystems

RNA Polymers - Poly rA. 2 Poly rU Triple-Stranded Polymer, sodium salt from Midland Certified Reagent Company

Description:... Polyribonucleotides (RNA polymers) are prepared by the catalytic action of the enzyme, polynucleotide phosphorylase, isolated at Midland, and the appropriate, ribonucleoside diphosphate as substrate. With the exception of catalog number P-3102, RNA pol...
Company:Midland Certified Reagent Company
Polynucleotide in Biological Definition

DNA

... referred to by the identity of their bases. These are adenine (A), thymine (T), cytosine (C), and guanine (G). In a DNA double helix, two polynucleotide strands can associate through the hydrophobic effect . Specificity of which strands stay associated is determined by complementary pairing . Each ba...
Polynucleotide in Biological Dictionary

Polynucleotide Kinase

... In enzymology, a polynucleotide 5'-hydroxyl- kinase (EC 2.7.1.78) is an enz... that catalyzes the chemical reaction Use this polynucleotide kinase to: phosphorylate 5_ -ends of DNA or RNA ... T4 polynucleotide Kinase ; 3'-phosphatase-free. Alkaline Phosphata...

Phosphodiester Bond

...NA polymerase get its energy to make phosphodiester bond ? ... phosphodiester bond n. The covalent chemical bond that holds together the polynucleotide chains of RNA and DNA by joining a carbon in the pentose The phosphate groups in the phosphodiester bond are negatively-charged. ... A phosphod...

Okazaki fragments

... singles, watch music ... Okazaki Fragments's Latest Blog Entry [Subscribe to this Blog] ... Details on Okazaki Fragments , DNA Polymerases, polynucleotide Ligases, Okazaki , Vitro Synthesis, Replication of DNA ... that Okazaki fragments are initiated ... The initiator RNAs of mammalian Okazaki ...

Kinase

...arge and important, but it is only one family in a ... Hence, the protein kinase -like superfamily has undergone substantial structural ... T4 polynucleotide Kinase catalyzes the transfer and exchange of P<sub>i</sub> from the γ position of ATP to the 5' -hydroxyl terminus of double- and singl...

Exonuclease

... Exonucleases are enzymes that work by cleaving nucleotides one at a time from the end of a polynucleotide chain. A hydrolyzing reaction occurs that breaks phosphodiester bonds at either the 3’ or 5’ ends. Its close relative... exonuclease activity....

Concatemer

...hod utilises a concatemer of the target polynucleotide, i.e. repeating ... [0050]The concatemer may be formed in any suitable way. ... ... each polynucleotide comprises a concatemer having multiple copies of a target ... distributed on the surface in a manner to promote the fixing of one concatemer ... ...

Codon

.... What is codon ? Meaning of codon medical term. What does codon mean? ... codon /co·don/ (ko'don) a series of three adjacent bases in one polynucleotide ... Codon . National Human Genome Research Institute (NHGRI) by artist Darryl Leja. Look up a description of Codon in the NHGRI Talking Glossary...

Base sequence

...ucture of the sequenced molecule. base sequence ( ′bās ′sēkwəns ) ( genetics ) The specific order of purine and pyrimidine bases in a polynucleotide such as deoxyribonucleic acid or sequence ( ) n. A following of one thing after another; succession. ... dna having the sequence a-g-g-c-g-...
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