Examination of internal 'wiring' of yeast, worm, and fly reveals conserved circuits

...d conservation and overlap in yeast, worm, and fly in 71 distinct "network regions" that included such essential functions as protein degradation, RNA polyadenylation and splicing, protein phosphorylation and signal transduction, DNA synthesis, nuclear-cytoplasmic transport, and protein folding. Such networks enrich...

Polyadenylation in Biological Technology

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...n RNA polymerase II-type CMV promoter (human cytomegalovirus immediate-early promoter) and an optimized SV40 polyadenylation signal to drive high-level expression of hairpin siRNA. For stable transfections requiring long-term antibio...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

...d widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This des...

Cis-Reporting System Monitors Intracellular Calcium Mobilization

...d widely used NFAT binding sequence. 1,2,10,11 In addition to a polyadenylation signal downstream of the luciferase gene, the pNFAT-Luc plasmid contains another polyadenylation signal in front of the NFAT binding sites and TATA box. This des...

Mammalian Expression Vector for Efficient Cloning of PCR Fragments

...gh efficiency and 1-hour ligation. However, the pCMV-Script vector has many distinguishing features. The powerful CMV promoter and the SV40 polyadenylation site allow high-level constitutive expression in a wide variety of cell lines. In addition, the neomycin-resistance gene is unde...

Mammalian and Bacterial Expression in One Vector

...expression vectors. Yet mammalian and bacterial plasmid vectors vary considerably in their choice of promoters, the presence of splice signals and polyadenylation sites, as well as their respective translation initiation sequences. In fact, the differences between bacterial and mammalian expression vectors a...

High-Titer Retroviral Vectors for Gene Delivery

... region contains the retroviral promoter/enhancer, and is flanked in the 3 direction by the R region, which contains the viral polyadenylation signal (pA). The R region is followed by the U5 region, which, along with R, contains sequences that are critical for reverse tr...

A New Lambda Vector for Mammalian Expression

...vector; 6 gene expression driven by the CMV promoter for constitutive expression in a wide variety of cell lines; ### the SV40 polyadenylation site; and the neomycin-resistance gene under control of the prokaryotic b -lactamase promoter, to provide kanamycin ...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... with a shuttle vector. Fig.2 The pShuttle-CMV vector contains a multiple cloning site between the CMV promoter and the SV40 polyadenylation signal. The pShuttle vector contains only a multiple cloning site in which an entire expression cassette can be inserted. The regions indicated as...

New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

...oth the pCMV-BD and pCMV-AD vectors. The SV40 poly (A) signal provides the signals necessary for transcriptional termination and polyadenylation of the bait and target genes in mammalian cells. Stratagene offers this system as a vector kit containing both the bait vector, target (prey) ve...

Polyadenylation in Biological Products

loxP flanked Ampicillin Selection Cassette (loxP-amp-loxP) from Gene Bridges GmbH

Description:...ance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by lox sites for later excision by Cre recombinase. Unique Not I and Xho I site...
Company:Gene Bridges GmbH

FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH

Description:...ance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique Not I and Xho I site...
Company:Gene Bridges GmbH

loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

Description:...ance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the chloramphenicol expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique Not I and Xho ...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH

Description:...ficiency than the generally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as the eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. An additional single...
Company:Gene Bridges GmbH

loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH

Description:...on efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by loxP sites for later excision by Cre recombinase. Unique Not I and X...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH

Description:...on efficiency than the normally used Tn5 promoter. The promoter of the mouse Phosphoglucokinase gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by FRT sites for later excision by Flp recombinase. Unique Not I and Xh...
Company:Gene Bridges GmbH

Polyadenylation in Biological Definition

Alternative splicing

...nylation sites : this is the only method of splicing which can produce an alternative C-terminus domain in proteins. In this case, different sets of polyadenylation sites can be spliced with the other exons. Intron retaining mode : in this case, instead of splicing out an intron, the intron is retained in the...

Polyadenylation in Biological Dictionary

Polyadenylation

Polyadenylation is the addition of a poly(A) tail, a stretch of RNA where all the bases are adenines, onto an RNA molecule. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for... Cleavage and polyadenylation specificity factor (CPSF) is inv...

Untranslated RNA

...NA. 3 RNA ... uracil is appropriate for RNA , where quantity is important but ... RNA is created in the transcription; after splicing and polyadenylation it is ... Untranslated regions ... DNA strand that the RNA was transcribed from. ... ...

Pre-mRNA

...ered at an early stage of spliceosome assembly. ... ... the structure is a good mimic of the phosphate group of the pre - mRNA substrate. ... polyadenylation factor CPSF-73 is the pre - mRNA 3'-end-processing endonuclease. ... Pre mRNA information including symptoms, causes, diseases, symptoms, tre...

PolyA tail

... polyadenylation is the addition of a poly(A ) tail , a stretch of RNA where all ... The poly(A ) tail is important for the nuclear export, translation and ...

MRNA processing

...A post-transcriptional processing is far from ... ... processing events include cleavage at a specific site in the pre- mRNA followed ... polyadenylation factor CPSF-73 is the pre- mRNA 3'-end- processing endonuclease. ... mRNA ... PRP18 pre- mRNA processing factor 18 homolog (S. cerevisiae) T...