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Polyacrylamide in Medical News

Breast Enlargement Injections Can Lead To Breast Loss

...r breasts// due to complications. Fifty-three women from Hong Kong have complained of complications such as infections and abscesses after having polyacrylamide gel injected into their breasts. Six women had to have parts of their breasts surgically removed after suffering severe complications from the o...
Polyacrylamide in Medical Products

GelAir™ Drying System (230 V, 50 Hz)

Description:... The GelAir™ drying system utilizes a hot air drying device for polyacrylamide and agarose gels. The dryer holds up to four drying frames at once. Gels are dried on a clear cellophane support for easy viewing. The assembly table ...
Company:Bio-Rad Laboratories

GelAir™ Drying System (115 V, 60 Hz)

Description:... The GelAir™ drying system utilizes a hot air drying device for polyacrylamide and agarose gels. Gels are dried on a clear cellophane support for easy viewing. The assembly table is recessed to provide a smooth, flat assembly sur...
Company:Bio-Rad Laboratories

Certified Low Range Ultra Agarose

Description:... and primers at lower concentrations than standard agaroses. A 3% gel clearly resolves a 10 bp ladder, and a 4% gel approaches the resolution of an 8% polyacrylamide gel....
Company:Bio-Rad Lab Equipment

Eppendorf Geloader Pipette Tips

Description:... This pipette tip facilitates the loading of samples onto polyacrylamide gels for DNA sequencing. The Geloader Tip has a 15 mm capillary with a defined diameter of less than 0.3 mm. The flexibility of the tip prevents the g...
Company:Brinkmann

ART GEL 20P

Description:... Perfect for loading small volume samples onto agarose and polyacrylamide gels....
Company:Molecular BioProducts

The Panther Semi-dry Electroblotter - Model HEP-1 (Complete System)

Description:... The HEP-1 system accommodates mini to full size gels with a transfer area of 20cmWx 20cmL, designed specifically for the transfer from polyacrylamide or agarose gels....
Company:Owl Separation Systems

CEQ 8800 Genetic Analysis System

Description:... The CEQ 8800 features unique DNA sequencing reagents including linear polyacrylamide gel (LPA), coated capillaries, ITP chemistry and cyanine-based infrared dyes- coupled with online denaturation. This results in less correction, ore ...
Company:Beckman Coulter, Inc.

CEQ 8000 Genetic Analysis System

Description:... CEQ 8000 is a fully automated genetic analysis system. This system automatically fills the capillary array with a patented linear polyacrylamide (LPA) gel, denatures and loads the sample, applies the voltage program, and analyzes the data. Software tools let you rapidly review data quality and ...
Company:Beckman Coulter, Inc.

QualiCode™ B. burgdorferi IgG/IgM Western Blot Kits

Description:...chete are inactivated by detergent disruption. Purified B. burgdorferi antigens are fractionated according to molecular weight by electrophoresis on a polyacrylamide slab gel in the presence of sodium dodecyl sulfate. The separated B. burgdorferi antigens are transferred via electrophoretic blotting from the gel to...
Company:Immunetics, Inc.

CEQ™ 8000 Genetic Analysis System

Description:... CEQ 8000 is a fully automated genetic analysis system. This system automatically fills the capillary array with a patented linear polyacrylamide (LPA) gel, denatures and loads the sample, applies the voltage program, and analyzes the data. Software tools let you rapidly review data quality and ...
Company:Beckman Coulter, Inc.
Polyacrylamide in Medical Dictionary

Protein electrophoresis

... a fixed amount ... In this experiment, total protein extracts from several bacterial sources ... of protein bands, obtained by SDS vertical polyacrylamide electrophoresis , can ... ...
Polyacrylamide in Biological News

Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols

...lue-Native PAGE," written by Harvey Millar and colleagues from the University of Western Australia, describes multiple experimental approaches using polyacrylamide gel electrophoresis (PAGE). Blue-native PAGE (BN-PAGE) allows a range of protein complexes to be visualized. When combined with sodium dodecyl sulfa...

UCR environmental scientists propose chemical solution to cleaning California's Salton Sea

... lead author of the paper. "We found that alum and polyacrylamide were highly effective in removing both dissolved p...ea in both colloidal and dissolved forms. Alum and polyacrylamide are commonly used in municipal wastewater treatmen...er coagulated suspended solids and settles out. polyacrylamide is a synthetic polymer used in soil applications t...
Polyacrylamide in Biological Technology

Caliper Life Sciences Introduces Staccato Protein Workstation for Automated Protein Sample Preparation & Analysis

...The LabChip GXII is Caliper's high throughput system for analyzing protein size, concentration, and purity and serves as a replacement for traditional polyacrylamide gel electrophoresis (PAGE). Since its launch in July, 2008, the LabChip GXII platform has been widely adopted by high throughput protein analysis labo...

LI-COR Introduces the Odyssey(R) Two-Color NIR Fluorescent Protein Molecular Weight Marker

...otein Molecular Weight Marker is pre-stained and fluoresces in the 700 and 800 nm channels of the Odyssey System. The marker was designed for use with polyacrylamide gels and can be used to visualize the progress of protein separation during electrophoresis, to monitor transfer efficiency to Western blot membrane, ...

Using catalysts to stamp nanopatterns without ink

...nstration, Toones team chose as a catalyst the biological enzyme exonuclease I, derived from the bacterium E. coli. In one set of experiments, the polyacrylamide stamp pattern bearing the tethered enzymes was then pressed on a surface of gold that had been covered with a uniform coating of single-stranded DNA m...

Evaluation of FlashPlate in a Helicase Assay

...ting replication and playing an important role in DNA repair. Conventional helicase functional assays require that unwound DNA products be analyzed on polyacrylamide gels, which have major constraints in high throughput screening (HTS) applications. Preparing the gels is a time-, labor-, and material-intensive task...

ECL Semi-dry Blotters

...vailable with and without built-in power supplies to give maximum flexibility • Complete, uniform electrotransfer of proteins from polyacrylamide gels in less than 1 h • Minimal buffer use—just enough to saturate blotting papers and membranes • Lon...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...fragment migrates more slowly through a polyacrylamide gel than does an undegraded, helical, doublestrand...ic strength of sample, etc.) than other polyacrylamide gel electrophoresis procedures. 7 To further shar...). The gel also contained a gradient of polyacrylamide ranging from 10 to 20%, made from a 40%...

Protein Truncation Tests Using the DCode System

...ions were performed in 12.5 ml with 6 Ci of 35 S-methionine. Protein products were run on a 16 x 20 cm, 14% SDS-PAGE denaturing polyacrylamide gel using the DCode universal mutation detection system. The samples were run at room temperature in 1x Tris/Glycine/SDS buffer ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

...trosospira. 2,3 Approximately 200 ng of PCR product were mixed with 2x bromophenol blue loading dye and electrophoresed in a 6% polyacrylamide gel (acrylamide/bis 37.5:1) containing 7 M urea, 20% formamide, 2% glycerol in 1.25x TAE buffer (50 mM Tris-Cl, 25 mM acetic aci...

Heteroduplex Analysis Using the DCode System

...altered conformation can be detected on polyacrylamide gels due to slower migration than the c...ity in a gel from homoduplex molecules. polyacrylamide analogs have been developed, such as DE...x samples when compared to conventional polyacrylamide gels. 2 In this experiment, we show tha...

Detection of Mutations in the CYP21 Gene Using the DCode System

... gel electrophoresis was carried out using the DCode system (Bio-Rad). Fifteen l of PCR product was loaded on a gel containing a polyacrylamide gradient ranging from 612% and a gradient of urea and formamide. The gels were run in 1x TAE buffer, at 80 V overnight. After el...
Polyacrylamide in Biological Products

PhastGel DNA Silver Staining Kit from GE Healthcare, formerly Amersham Biosciences

Description:... PhastGel DNA Silver Staining Kit, 1. Select from homogeneous and gradient polyacrylamide gels.Separate proteins and peptides on denaturing, native, and isoelectric focusing gels.Save time and improve reproducibility with precast gels, buff...
Company:GE Healthcare, formerly Amersham Biosciences

PlusOne Silver Staining Kit; Protein from GE Healthcare, formerly Amersham Biosciences

Description:...s backgrounds in most gel electrophoresis systems.Silver Staining Kit, Protein provides convenient, reproducible and sensitive staining of proteins in polyacrylamide gels in less than two hours.The visualization technique detects most proteins in the nanogram range, which is 100-fold more sensitive than Coomassie B...
Company:GE Healthcare, formerly Amersham Biosciences

1-D PAGE Cleavable ICAT Reagent Applications Development Kit for Targeted Protein ID and Quantitation (monoplex Version) from Applied Biosystems

Description:... The 1-D PAGE Cleavable ICAT Reagent Application Kit for Targeted Protein ID and Quantitation combines SDS polyacrylamide Gel Electrophoresis (PAGE), isotope-coded affinity tag chemistry, and cleavable-linker technology to facilitate the identification and quantification ...
Company:Applied Biosystems

10X BlueJuice Gel Loading Buffer from Invitrogen

Description:... 10X BlueJuice Gel Loading Buffer is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels. This loading buffer features higher density sucrose which allows for easier pipetting than glycerol-based loading buffers....
Company:Invitrogen

dsRNA Marker from Abnova Corporation

Description:...A band including in the dsRNA Marker is adjusted to approximately 25 ng/µl in concentration. The dsRNA Marker is manufactured for non-denaturing polyacrylamide gel electrophoresis. The dsRNA Marker can be visualized by UV light after ethidium bromide staining....
Company:Abnova Corporation

Protein Staining Solution from Abnova Corporation

Description:... Protein Staining Solution stains proteins in polyacrylamide gel after SDS gel electrophoresis (detection limit >= 8 ng of protein) with *CoomassieR-G250. All processes including washing and destaining processes...
Company:Abnova Corporation

2% Bis Solution, 500 ml from Bio-Rad

Description:... Bis-Acrylamide (bis) is a monomer used as a crosslinker with acrylamide to form polyacrylamide electrophoretic gels. This 2% bis solution is supplied as 500 ml....
Company:Bio-Rad

Gel Loading Solution from Sigma-Aldrich

Description:... Gel loading buffer is especially formulated for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids. The gel loading solution contains a bromophenol blue tracking dye and sucrose to add density and fac...
Company:Sigma-Aldrich

Gel Loading Solution (Type I) from Sigma-Aldrich

Description:... Gel loading solution, Type I is formulated for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids and offers the advantage of two tracking dyes (bromphenol blue and xylene cyanole). Sucrose adds dens...
Company:Sigma-Aldrich

Acridine Orange hemi(zinc chloride) salt from Sigma-Aldrich

Description:... Application: Acridine orange has been used as a fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Acridine orange at a concentration of 120 μM will detect purified DNA in gels with a sensitivity of 25-50 ng per band. It has also been use...
Company:Sigma-Aldrich
Polyacrylamide in Biological Definition

Gel electrophoresis

...nker , producing different sized mesh networks of polyacrylamide . When separating larger nucleic acids (greater th...ain termination method " page for an example of a polyacrylamide DNA sequencing gel. Gel electrophoresis of proteins is usually done in an SDS polyacrylamide gel ( SDS-PAGE ), by native gel electrophoresis , ...

Gel electrophoresis

...nker , producing different sized mesh networks of polyacrylamide . When separating larger nucleic acids (greater th...ain termination method " page for an example of a polyacrylamide DNA sequencing gel. Gel electrophoresis of proteins is usually done in an SDS polyacrylamide gel ( SDS-PAGE ), by native gel electrophoresis , ...
Polyacrylamide in Biological Dictionary

SDS-PAGE

... SDS - PAGE , sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique widely used ...to their electrophoretic mobility .The... SDS polyacrylamide gel electrophoresis ( ¦es¦dē¦es ′pälēə¦kriləmī...proteins. ... SDS - PAGE , sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a ... SDS - PAGE , o...

Primer extension

...he Primer Extension System is used to determine the location and level of a ... Primer extension products are typically analyzed on denaturing polyacrylamide ... Primer extension analysis is used to determine the location and to quantitate ... RNA recommended for the primer extension reaction dep...

Polyacrylamide Gel

... A polyacrylamide Gel is a separation matrix used in electrophores...ques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a sin...ethods now... SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, is a technique widely used...

Native Gel

...ed and therefore separated based on their charge-to-mass ratio. Native polyacrylamide gel electrophoresis is an electrophoretic separation method typically use...Mini gel with 0.75 mm spacer. Sample (4%) for 2 gels ... Blue Native polyacrylamide gel electrophoresis (BNPAGE) is ... III. NATIVE ACRYLAMIDE GEL PREP...

Gel electrophoresis

...ectrophoresis is an important and widely used tool in the analysis of biological ... mance of a wet-lab gel electrophoresis activity. ... "SDS polyacrylamide gel electrophoresis "; 2000; J. V. Maizel, Jr.;Trends ... Detail: Novel denaturing gradient gel electrophoresis methods are described. ... ...

DNA sequencing

...enome was sequenced in the Human Genome Project? ... ... DNA polymerase-catalyzed radiolabeled two-step sequencing ... DNA sequencing : ... polyacrylamide gels for DNA sequencing , the appropriate amount ... DNA . Sequencing . 59. With the introduction of the bench-top Genome Analyzer System, eve...

DNA sequence

...Yourself | Journey into DNA | Meet the Decoders ... ... DNA polymerase-catalyzed radiolabeled two-step sequencing ... DNA sequencing : ... polyacrylamide gels for DNA sequencing , the appropriate amount ... Dna Sequencing : Single Molecule Sequencing resources and information at dnasequencing...

Denaturing Gel

... such a technique ... PURIFICATIONOF OLIGONUCLEOTIDES USING DENATURING polyacrylamide GEL ELECTROPHORESIS ... gel during electrophoresisis desirable since it...orld supplier of reagents and reagent systems for the life ... Denaturing polyacrylamide Gel Electrophoresis of DNA & RNA ... ...

Agarose gels

...A from agarose gels , and choosing between them is, to some ... out the desired piece of agarose using a razor blade or ... Both agarose and polyacrylamide gels can be used for separation of double ... DNA electrophoresis in agarose gels at alkaline pH is also used to separate ... Agarose ge...

Acrylamide gels

... A polyacrylamide Gel is a separation matrix used in electrophoresi...iques such as Maxam-Gilbert or Sanger methods used polyacrylamide gels to separate DNA fragments differing by a si...for protein analysis, they are ... ... offers polyacrylamide precast gels for electrophoresis of ... For a...
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