Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

... The following laboratory equipment is required: ◊ pixcell IIe LCM Instrument equipped with Fluor 300 epifluorescence optics optimiz... emission filters with an integration setting of 0.12-0.2 using an Arcturus pixcell IIe system equipped with epifluorescence optics. 5. A spot size...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... ◊ Cryostat knife ◊ Arcturus pixcell II Laser Capture Microdissection System with fl...boratory bench surfaces, cryostat, cryostat knife, pixcell II Laser Capture Microdissection System and Arct...apture microdissection. 10. The Arcturus pixcell II Laser Capture Microdissection System with flu...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...II. LASER CAPTURE MICRODISSECTION ◊ pixcell IIe with fluorescence (Arcturus, Cat. #LCM1104) ...ide was thawed. CapSure HS LCM caps were used with pixcell IIe settings as follows: 450-700 s duration, 60-85... 1500-2000 of these neurons one-by-one using a pixcell IIe (Fig. 1 a-e). RNA from these captures was po...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...es, Cat. #SK-5100) III. LASER CAPTURE MICRODISSECTION ◊ pixcell II Laser Capture Microdissection Instrument (Arcturus, Cat. #LCM1104) ...ce. Approximately 300 laser pulses were performed on each section using the pixcell II LCM Instrument. The following parameters were used: 15m laser diameter, ...

RNA from LCM Samples

...Brain. (A) Cryosection (10 micron) stained with cresyl violet. (B) Same section after Laser Capture Microdissection using pixcell IIe LCM workstation (Arcturus). Note absence of target structure (dentate gyrus). (C) Microdissected dentate gyrus adhered to ...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

...samples were obtained from 10 m sections of mouse brain with the pixcell IIe LCM System (Arcturus). Two rounds of RNA amplification were ...dine Orange (LCM Staining Kit, Ambion). Microdissection with the pixcell IIe LCM System (Arcturus) was performed with the lowest settings...

PixCell in Biological Products

PixCell IIe LCM Instrument with PixCell II Image Archiving Workstation (220 volts) from Arcturus, now part of Molecular Devices

Description: Obtain Clean and Accurate Results Laser Capture Microdissection (LCM) enables you to dramatically increase the sensitivity and accuracy of molecular assays by starting with samples of homogeneous cell types and multi-cellular structures isolated from whole tissue or cytology samples. This unique ...
Company:Arcturus, now part of Molecular Devices

PixCell IIe LCM Instrument with PixCell II Image Archiving Workstation (110 volts) from Arcturus, now part of Molecular Devices

Description: Obtain Clean and Accurate Results Laser Capture Microdissection (LCM) enables you to dramatically increase the sensitivity and accuracy of molecular assays by starting with samples of homogeneous cell types and multi-cellular structures isolated from whole tissue or cytology samples. This unique ...
Company:Arcturus, now part of Molecular Devices