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Photobleaching in Medical News

New laser method reproduces art masterworks to protein patterns

...ir." Flexible and precise Using laser-assisted protein adsorption by photobleaching (LAPAP), the scientific team bound fluorescently-tagged molecules to a glas...science. "Our next goal is to extend laser-assisted protein adsorption by photobleaching to fabricate more complex protein combinations and distributions," adds Dr....

Bad to the bone: UD research to shed light on osteoporosis

...g method that Wang developed, which is based on fluorescence recovery after photobleaching (FRAP), the research team hopes to do what no one has done before: see insi...e dyed molecules will be subjected to a flash of intense light, temporarily photobleaching them black, leaving a distinct black sector surrounded by green. Thus, if t...

Helicobacter pylori inhibits intercellular communication of cultured gastric cells

...d negative (CagA-), respectively. After overnight treatment, the GJIC of the cells was measured by a technique named fluorescence redistribution after photobleaching (FRAP). The authors found that both CagA+ and CagA- H pylori strains could inhibit the GJIC of gastric cells when compared with a blank group. In addi...
Photobleaching in Biological News

Basic guides to PCR, labeling neurons featured in Cold Spring Harbor Protocols

...d dye-labeled proteins for fluorescence resonance energy transfer (FRET), fluorescence correlation spectroscopy (FCS), and fluorescence recovery after photobleaching (FRAP) studies. The most widespread application of this technique has been for Ca2+ imaging. This method is freely accessible on the website for Col...

Media highlights in the March 1 issue of Biophysical Journal

...ponents as a function of time. Another advantage is that immobile or slowly moving features, like cell edges and borders as well as contributions from photobleaching are separated so that only the species that fluctuate more rapidly are analyzed. This technique is can be used with laser scanning microscopes and is ...

Research sheds light on the mechanics of gene transcription

...er after heat shock. As in the polytene chromosomes, the genes did not move to a single site for transcription. And using fluorescence recovery after photobleaching -- another method engineered by Webb -- the researchers found that over time Pol II began to recycle itself within newly formed "compartments" around ...

Imaging techniques permit scientists to follow a day -- or four -- in the life of a cell

...hnique is similar to those that are used to create cloned and transgenic organisms, and it can also be applied to more complex assays--including laser photobleaching and fluorescent speckle microscopy--that yield specific insights into cellular processes. A second freely available protocol ( http://www.cshprot...

Nanoscale method for investigating living systems

...de via voltage." Quantum dots can be encoded with different colors making them useful as fluorescent labels for staining cells. Their resistance to photobleaching and physical size of less than 10 nanometers are making them increasingly popular in biomedical applications ranging from intracellular tagging of mol...

Quantum Dots Research Leads to New Knowledge about Protein Binding in Plants

...dhesin (SCA) - a plant pollen tube adhesion protein. This labeled the protein molecules with fluorescent tags. Quantum dots are much more resistant to photobleaching than conventional fluorescent markers and their small size make them ideal for biological imaging. The researchers then added the molecules to germina...
Photobleaching in Biological Technology

Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer

Paul Gavin*, Mark Prescott*, Ph.D, Daren J. Fyfe#, Ph.D and Jeffrey J. Comerford#, Ph.D * Department of Biochemistry and Molecular Biology, Monash University, Clayton campus, Victoria 3800, Australia # Technical Assist...

AMG Launches EVOS fl, an 'Evolutionary' Fluorescence Microscope Based on Innovative EVOS Platform

...0 hours, the user never has to change a bulb. Most importantly, LEDs can be instantly adjusted and precisely controlled by the user, so the chance of photobleaching or phototoxicity is greatly reduced. The EVOS fl is supported by an embedded microprocessor that gives the user full control of image acquisiti...

Nikon Instruments A1 Confocal Laser Microscope Series With NIS-Elements C Software Delivers Fully Integrated Comprehensive Confocal Imaging Capabilities

...riment work flow and secure data acquisition, storage and analysis. NIS-Elements C makes observing live changes and graphing intensity during photobleaching and photoactivation easy with a well-developed and purposeful monitoring interface. Also, maximum and minimum intensity projections over time and Z-a...

Nikon Instruments' New NIS-Elements C Delivers Comprehensive Confocal and Widefield Imaging Capabilities in Single Unifying Software Platform

...p, experiment work flow and secure data acquisition, storage and analysis. NIS-Elements C makes observing live changes and graphing intensity during photobleaching and photoactivation easy with a well-developed and purposeful monitoring interface. Also, maximum and minimum intensity projections over time and Z-ax...

Nikon Instruments Introduces NIS-Elements Software for Eclipse Ti-E Live-Cell Applications

...Elements' latest version enables exceptional image data throughput and multi-dimensional image capture routines that advance productivity and minimize photobleaching and cell phototoxicity. The fully-integrated system, which is ideal for high-end research and live-cell imaging, enables extremely fast image sequence...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

...signal-to-noise ratio than a conventional widefield microscope. The point-illumination scanning scheme does reduce the lateral background, and reduces photobleaching in the specimen. As the brightfield mode is not implemented in a confocal configuration, it is only suitable for applications in thin and stained tiss...

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse

...ternal filters (on both excitation and emission monochromators) and the Cary Eclipse xenon flash lamp respectively. The issue of photobleaching is extremely important and is discussed in a separate application note. The data indicate (Figure 3) that FRET could be accuratel...

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

...tein, as a reporter gene in Jurkat cells. EGFP has an excitation maximum at 488 nm, and emission maximum at 507 nm. It is very resistant to photobleaching and is reported to maintain stable fluorescence from pH 7.0 to 11.5. 3 In fluorometric assays, purified EGFP can be measured at...
Photobleaching in Biological Products

SlowFade Light Antifade Kit from Molecular Probes (Invitrogen)

Description:... The SlowFade Light Antifade Kit is an aqueous-based mounting reagent that reduces the photobleaching seen with fluorescent dyes. This formulation of antifade minimizes the initial quenching of fluorescein AMCA and Cascade Blue fluorophores that is oft...
Company:Molecular Probes (Invitrogen)

Fluorescent Mounting Media from KPL

Description:... Glycerol-based mounting medium for immunofluorescence microscopy. Formulated to retard photobleaching of fluorochromes such as fluorescein and rhodamine. Product can also be used as a diluent for fluorescent-labeled conjugates....
Company:KPL

FluoroGuard Antifade Reagent from Bio-Rad

Description:... FluoroGuard antifade reagent is used to inhibit photobleaching in confocal and standard fluorescence microscopy. The unique formulation effectively suppresses photobleaching in a wide variety of fixed specimens stained or labeled with fluorescein, r...
Company:Bio-Rad

VersArray ChipReader 3 m System from Bio-Rad

Description:...the range for increased sensitivity. Results with VersArray ChipReader systems have a low background, enhanced weak signal detection, and virtually no photobleaching for a long dye lifetime....
Company:Bio-Rad

VersArray ChipReader 5 m System from Bio-Rad

Description:...the range for increased sensitivity. Results with VersArray ChipReader systems have a low background, enhanced weak signal detection, and virtually no photobleaching for a long dye lifetime. You also get real-time feedback on the effects of laser and photomultiplier tube adjustment on the signal-to-noise ratio and ...
Company:Bio-Rad

BD CARV II Confocal Imager from BD Biosciences - Bioimaging

Description:...ocal scanning, combined with high quantum efficiency CCD cameras, minimizes photobleaching and allows real-time imaging and recording at up to 100 fps. A long life ar... spectrum confocal Automated filter selection Fluorescence Recovery After photobleaching ( FRAP) capabilities Microscope compatibility Application specific camera...
Company:BD Biosciences - Bioimaging
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