....See also: Bulla ... In many cases, vesicles break easily and release their fluid onto the skin. ... Synapsins are synaptic- vesicle -associated phosphoproteins
implicated in the ... Turnover of transmitter and synaptic vesicles at the frog neuromuscular junction. ... ... and found in the media as part o...
purification. After disruption using the TissueRuptor, phosphoproteins
were successfully purified from animal tissue (Figure 5), with phosphoproteins
detected only in the eluate.
Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
...h linearity over 3.6 orders of magnitude. Two antigens can be detected simultaneously, and differential quantitation of low-abundant proteins, such as phosphoproteins
in cell lysates, can be performed.
ECL Plex Western blotting detection system is based on direct f...
2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
...und originating from 50 proteins. Some of the identified phosphorylation sites are shown in Table 1. The proteins presented in the table are all known phosphoproteins
involved in cell growth and cell differentiation.
MS/MS and MS 3 spectra of one previously known phospho-peptide originat...
Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
...nal for total ERK2, though not linear, showed
a good dose-response over the range from 12 to 0.6 g/ml. The same was
true for the phosphoproteins
in each of the treated samples. These results
emphasize the importance of establishing the dose-response relationship
Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
...hr 183 /Tyr 185 ), p38 MAPK (Thr 180 /Tyr 182 ).
Quantitation of phosphoproteins
The western blot signals for each phosphoprotein were quantita...ern
blotting and Bio-Plex multiplex suspension array analysis of phosphoproteins
with the same cell extracts.
TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
phosphorylated when purified from TKB1 but were not phosphorylated from
the parental BL21 strain ( Figure
3 ). The phosphoproteins
found in the TKB1 cells expressing gT-F3 likely
represented contaminating bacterial proteins, as these have not been repeatedly