Pharmexa and Affitech to Combine Operations to Create a New Antibody Therapeutics Company

...ented: "Affitech has developed a fully integrated antibody discovery platform consisting of human antibody libraries with high functionality, based on phagemid display technology plus several screening technologies with high degree of versatility. Through partnerships with both pharma and biotech businesses, ...

Using living cells as nanotechnology factories

...virus-like particle that infects a bacteria cell. Once inside the cell, the phagemid used the cell just like a photocopier machine to reproduce millions of copies of the DNA. By theoretically starting with just a single phagemid infection, and a single milliliter of cultured cells, Yan found that the ce...

M13/Phagemid DNA Extraction Protocol

...t agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

M13/Phagemid DNA Extraction Protocol

...t agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

A New Lambda Vector for Mammalian Expression

... Lambda ZAP II vector and the pCMV-Script -EX phagemid vector. High-efficiency cDNA libraries construct...ryotic expression in the pCMV-Script-EX phagemid vector. The Lambda ZAP-CMV vector ( Fig...le cloning site, and the pCMV-Script-EX phagemid vector. Figure ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

...at models the use of restriction enzymes is the blue-white color selection assay. In this assay, Stratagenes pBluescript II SK phagemid vector ( Figure 3 ), which contains the lacZ reporter gene, is cut at a single position within the multiple cloning site using a...

Purified Single-Stranded M13 DNA for Automated Sequencing

...nd realize high-quality automated fluorescent sequencing results as well. Additionally, the kit can be used to purify single-stranded DNA from rescued phagemid clones. REFERENCES Richards, S., et al. (1994) In Automated DNA Sequencing and Analysis (Eds. Adams, M.D., et al.) pp. 191-198. Ac...

Phagemid in Biological Products

Kilo Sequencing Deletion Kit from Takara Mirus Bio

Description:...d to aid sequence analysis of long DNA fragments cloned into the multiple cloning sites (MCS) of M13 phage vectors (mp18/19) or pUC-related plasmid or phagemid vectors (pUC18/19). The kit is used to create nested unidirectional deletions in the target DNA, thereby progressively moving the primer binding site ...
Company:Takara Mirus Bio

Phagemid in Biological Definition

Phage

...that are extensively studied: λ phage - Lysogen T4 phage (169 to 170 kbp , 200 nm long) T7 phage R17 phage M13 phage - phagemid See also Horizontal gene transfer One-step growth curve ...

Phagemid in Biological Dictionary

Phagemid

A phagemid or phasmid is a type of cloning vector developed as a hybrid of the filamentous phage M13 and plasmids to produce a vector that can grow as a plasmid, and also be packaged as single stranded DNA in viral particles. Phagemids contain an origin of replication (ori) for dou...