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Phagemid in Biological Technology

Pharmexa and Affitech to Combine Operations to Create a New Antibody Therapeutics Company

...ented: "Affitech has developed a fully integrated antibody discovery platform consisting of human antibody libraries with high functionality, based on phagemid display technology plus several screening technologies with high degree of versatility. Through partnerships with both pharma and biotech businesses, ...

Using living cells as nanotechnology factories

...virus-like particle that infects a bacteria cell. Once inside the cell, the phagemid used the cell just like a photocopier machine to reproduce millions of copies of the DNA. By theoretically starting with just a single phagemid infection, and a single milliliter of cultured cells, Yan found that the ce...

M13/Phagemid DNA Extraction Protocol

...t agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

M13/Phagemid DNA Extraction Protocol

...t agitation for good aeration of the culture. For producing phagemid ssDNA, suspend a colony of phagemid transformed bacteria in a 15 ml culture tube containing 3 ...

TripleMaster PCR System

... high fidelity amplifications of smaller targets ranging from 100 bp to 10 kb with genomic templates and up to 15 kb with plasmid or phagemid DNA. Control reagents are supplied with the TripleMaster PCR System Plus for the amplification of a 40 kb fragment. ...

A New Lambda Vector for Mammalian Expression

... Lambda ZAP II vector and the pCMV-Script -EX phagemid vector. High-efficiency cDNA libraries construct...ryotic expression in the pCMV-Script-EX phagemid vector. The Lambda ZAP-CMV vector ( Fig...le cloning site, and the pCMV-Script-EX phagemid vector. Figure ...

Restriction Endonucleases Undergo Rigorous QC Testing to Ensure Optimal Performance

...at models the use of restriction enzymes is the blue-white color selection assay. In this assay, Stratagenes pBluescript II SK phagemid vector ( Figure 3 ), which contains the lacZ reporter gene, is cut at a single position within the multiple cloning site using a...

Purified Single-Stranded M13 DNA for Automated Sequencing

...nd realize high-quality automated fluorescent sequencing results as well. Additionally, the kit can be used to purify single-stranded DNA from rescued phagemid clones. REFERENCES Richards, S., et al. (1994) In Automated DNA Sequencing and Analysis (Eds. Adams, M.D., et al.) pp. 191-198. Ac...
Phagemid in Biological Products

Kilo Sequencing Deletion Kit from Takara Mirus Bio

Description:...d to aid sequence analysis of long DNA fragments cloned into the multiple cloning sites (MCS) of M13 phage vectors (mp18/19) or pUC-related plasmid or phagemid vectors (pUC18/19). The kit is used to create nested unidirectional deletions in the target DNA, thereby progressively moving the primer binding site ...
Company:Takara Mirus Bio
Phagemid in Biological Definition

Phage

...that are extensively studied: λ phage - Lysogen T4 phage (169 to 170 kbp , 200 nm long) T7 phage R17 phage M13 phage - phagemid See also Horizontal gene transfer One-step growth curve ...
Phagemid in Biological Dictionary

Phagemid

A phagemid or phasmid is a type of cloning vector developed as a hybrid of the filamentous phage M13 and plasmids to produce a vector that can grow as a plasmid, and also be packaged as single stranded DNA in viral particles. Phagemids contain an origin of replication (ori) for dou...
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(Date:4/22/2014)... German . , ... their mode of action is complex: In low concentrations ... while at high concentrations they can cause "oxidative stress", ... redox signaling unfolds in single cells and organelles in ... jointly by the teams of LMU Professor Martin Kerschensteiner ...
(Date:4/22/2014)... (SALT LAKE CITY)A computational tool developed at the University ... with unknown gene mutations in three separate cases, U ... new study in The American Journal of Human ... Re-ranking tool), identifies undiagnosed illnesses and unknown gene mutations ... proteins that code for genes are made, in individual ...
(Date:4/22/2014)... nanodot technology, Berkeley Lab researchers have demonstrated the ... used to study the membranes of living cells. ... structures can reveal information critical to whether a ... cancerous, that can,t be obtained through conventional microscopy. ... provide a physical means to both probe and ...
(Date:4/22/2014)... -- In parallel with modern man ( Homo sapiens ), ... we lived side by side, such as Neanderthals and the ... sapiens survived. What was it in our genetic makeup ... little is known about our unique genetic makeup as distinguished ... fact that we are the only species among them ,to ...
(Date:4/22/2014)... may throw tantrums like toddlers, but their total ... say, a gerbil or fox squirrel, according to ... and birds ranging from orangutans to zebra finches. ... Yale and more than two-dozen other research institutions ... evolution of self-control, defined in the study as ...
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(Date:4/22/2014)... Winsor Lowe, interim director of the University of Montana,s ... in the Proceedings of the National Academy of Sciences ... network. , ... the U.S. Geological Survey, the University of Washington, the Cary ... U.S. Forest Service Northern Research Station examined 664 water samples ...
(Date:4/22/2014)... Scientists at the University of Massachusetts Amherst and the ... week report that they have discovered a new genus ... the Negro River in the Amazonia State of Brazil. ... Adlia Nogueira and Jos Antnio Alves-Gomes of INPA, describe ... the journal Proceedings of the Natural Sciences of ...
(Date:4/22/2014)... Mass. Analyzing thousands of breeding bird surveys sent ... and Canada over 35 years, wildlife researchers report that ... either northward or toward higher elevation in response to ... This means that most previous studies of potential climate ... factor or the other have likely underestimated the effects ...
Breaking Biology News(10 mins):New research focuses on streamwater chemistry, landscape variation 2New electric fish genus and species discovered in Brazil's Rio Negro 2Wildlife response to climate change is likely underestimated, experts warn 2
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