Optimizing pfuturbo DNA Polymerase Amplification Reactions with
Perfect Match PCR Enhancer
Improve PCR product yield and specificity for difficult amplifications
DNA Polymerase Amplification Reactions with
Perfect Match PCR Enhancer
Frances Bai Holly Hogrefe
Certain PCR amplification systems produce multiple product bands or
Enhanced Amplification of Long Targets with PfuTurbo DNA
High-fidelity PCR of genomic targets up to 19 kb
Enhanced Amplification of Long Targets with PfuTurbo
Michael Borns Holly Hogrefe
Improve amplification of long targets with PfuTurbo
DNA polymerase* by making simple modifications to PCR reacti...
PCR Performance Comparisons Between pfuturbo and Taq DNA
PCR Performance Comparisons Between PfuTurbo
and Taq DNA Polymerases
DNA polymerase is the superior choice for robust
high-fidelity PCR amplification
Holly Hogrefe Frances Bai Janice Cline
In this update, we compare the PCR performan...
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
...erformed with a Bio-Rad Gene Cycler using PfuTM or pfuturbo
Two different primer pairs,...l containing 2.5 units
of cloned Pfu or pfuturbo
(DNA polymerase Strategene, San Diego, CA),
...mize non-specific annealing of the primers, Pfu or pfuturbo
was added after the first de...
The TripleMaster PCR System for three different amplifications
...acy that is normally
only achieved using products that are specifically designed for high-fidelity
applications. Although the tested pfuturbo
System (Stratagene) is revealed
to have the lowest error rate, it is suitable only for the amplification
of very short sequences (< ...
Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR
polymerases except Stratagenes Pfu and pfuturbo
DNA polymerases. * ,
Herculase enhance...ercial proofreading DNA polymerases except Pfu and pfuturbo
DNA polymerases ( Figure
7 )...r than that of Herculase DNA polymerase.
remains the best choice for routine PCR applicatio...
Unique DNA Polymerase Formulation Excels in a Broad Range of PCR
... Enhanced DNA Polymerase
... ( Std. dev.)
...found to be 2.1-fold higher than the error rate of pfuturbo
DNA polymerase and 1.6-fold and 3.7-fol...
Performance Comparisons of Commercial RT-PCR Systems
... The ProSTAR ultra HF RT-PCR system,
DNA polymerase** and a versatile
versatile two-step format is used, which features pfuturbo
polymerase, 3 a special formulation of Pfu ... HF RT-PCR system is related to the
DNA polymerase for cDNA amplification. Additional ...
pfuturbo DNA Polymerase:
A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
...s that are easily cloned with high efficiency
A High-Performance, High-Fideli...loramphenicol resistance (CAM) gene generated from pfuturbo
DNA polymerase * , was cloned into the PCR-Sc...equent cloning.
Stratagene recently introduced pfuturbo
DNA polymerase, 1 a
special formulation of clon...
prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
.... The ProSTAR Ultra HF RT-PCR system
DNA polymerase * for amplifying
cDNAs with the...ons (10 6 -fold amplification)
using Stratagenes pfuturbo
DNA polymerase (error rate of 1.3 x 10 -6
M.F./...nal clones would contain mutations
introduced by pfuturbo
DNA polymerase [(1.3 x 10 -6 )(1000