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Permeabilization in Medical News

Nuvo's High Throughput Experimentation Platform Selected as 2009 Edison Award Finalist

...n the traditional method of measuring the permeabilizing effect of a drug formulation. INSIGHT's key technological innovation is that it measures skin permeabilization indirectly, via changes in the skin's electrical impedance caused by application of a formulation. This dramatically speeds the measurement process an...
Permeabilization in Biological News

Researchers from the UGR use a bacterium to obtain biopreservatives from food

...y, and extend the average duration of many foods for centuries. The work presented in the doctoral thesis approaches the molecular aspects of the permeabilization that AS-48 exerts on bacterial membranes. "We designed a strategy to carry out site-directed mutagenesis on the as-48A gene, the one which generates e...
Permeabilization in Biological Technology

Quantitative Analysis of the Cell Cycle in Imaging Applications

...n, washed with BD Perm/Wash Buffer (PBS with 0.1% saponin and 3% fetal calf serum, FCS, Cat. No. 552598b), and permeabilized again in BD CytoPerm Plus permeabilization Buffer (BD 552598c) for 10 min. Afterwards, they were washed with the Perm/Wash Buffer, and re-fixed for 5 minutes. Cells were then washed with Perm/W...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

... cells. In contrast, the measurement of intracellular proteins requires permeabilization and fixation of cells prior to antibody labeling. Therefore, staining w...er) and incubate 10 minutes at RT. 5. Two alternative steps, if permeabilization and fixation is done without BD permeabilizing solution 2: Resuspend ...
Permeabilization in Biological Products

LEUCOPERM Suspension fixation and permeabilization solution from GeneTex

Description: Reagent A - Fixation medium Reagent B - Permeabilisation medium LEUCOPERM reagents are intended for fixing cells in suspension with Reagent A and then permeabilising the cells with Reagent B. This procedure gives antibodies access to intracellular structures and leaves the morphological scatter ch...
Company:GeneTex

eBioscience IC Fixation Buffer 125 ml from eBioscience

Description:...ation Buffer, used in conjunction with eBioscience permeabilization Buffer (Cat. No. 00-8333), can be used to fix and ... Buffer is used first in this protocol followed by permeabilization Buffer. All washing steps, antibody dilutions, and incubations after cell permeabilization should be performed using the...
Company:eBioscience

BD Phosflow Perm Buffer III from BD Biosciences Pharmingen

Description:... Perm Buffer III is intented for permeabilization following fixation of PBMCs (or lysed whole blood) for staining for intracellular kinases and appropriate CD markers simultaneously. Applications: In...
Company:BD Biosciences Pharmingen

BD Phosflow Fix Buffer I from BD Biosciences Pharmingen

Description:... The BD PhosFlow Fix Buffer I can be used for the simultaneous fixation and permeabilization of cells prior to intracellular staining. Applications: Intracellular Staining (Flow) Storage Temperature: Refrigerate(2 to 8C)...
Company:BD Biosciences Pharmingen

SelectFX Alexa Fluor 488 Cytochrome c Apoptosis Detection Kit - for fixed cells from Molecular Probes (Invitrogen)

Description:...bright green fluorescence that is compatible with filters and instrument settings appropriate for fluorescein. The kit also includes cell fixative and permeabilization reagents as well as protocols for mammalian cell preparation and staining....
Company:Molecular Probes (Invitrogen)

BD Phosflow Perm/Wash Buffer I from BD Biosciences Pharmingen

Description:...nslationally modified signaling proteins to permeabilize cells and to serve as an antibody diluent and cell wash buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining. Applications: Intracellular Stai...
Company:BD Biosciences Pharmingen

FAM FLISP™ Lys-CMK Assay Kit from B-Bridge International

Description:...h SR-101 Labeled Substrates (red fluorescence; Ex=590 nm, Em=620 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activi...
Company:B-Bridge International

SR-101 FLISP FLISP Leu-CMK Assay Kit from B-Bridge International

Description:...h SR-101 Labeled Substrates (red fluorescence; Ex=590 nm, Em=620 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activi...
Company:B-Bridge International

FAM FLISP™ Leu-DAP Assay Kit from B-Bridge International

Description:...ith FAM-Labeled Substrate (green fluorescence; Ex=488 nm, Em=530 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activi...
Company:B-Bridge International

FAM FLISP™ Spacer-Leu-CMK Assay Kit from B-Bridge International

Description:...ith FAM-Labeled Substrate (green fluorescence; Ex=488 nm, Em=530 nm) • Accurately measure serine protease activation • No lysing, fixing, or permeabilization • B-Bridge's FLISP Kits utilize cell-permeable, fluorescently labeled inhibitors of serine proteases (FLISPs) to measure chymotrypsin-like activi...
Company:B-Bridge International
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