Navigation Links
PVDF in Biological Technology

Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

Eric Larson, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Introduction PVDF membrane was originally introduced to protein use as an ideal medium for the harsh chemical environment of N-terminal, o...

GEMS Introduces Ultrasonic Continuous Liquid Level Sensor for Challenging Fluid Measurement

... challenging fluid applications. The UCL-520 is a two-wire ultrasonic transmitter for measurement up to 26.2' (8m) and is built with a rugged pvdf transducer for challenging ultra pure, corrosive or waste liquids. The highly accurate sensor is easy to install within limited space applications and...

LI-COR Updates Product and Application Guide for Summer 2008

..., signal transduction pathway analysis, and time course studies. However, it is possible to adapt nearly all common Western procedures that generate a pvdf or Nitrocellulose blot of 7 x 8.5 cm to the MPX format. The new Pearl(TM) In Vivo Small Animal Imaging system is optimized for use with LI-COR IRDye...

LI-COR(R) Biosciences Introduces New Tools for High Throughput Western Blotting

...tibody screening, monoclonal antibody screening, signal transduction pathway analysis, or any other common Western blotting procedure that generates a pvdf or nitrocellulose blot of 7 x 8.5 cm. Processing is as simple as clamping the blot into the MPX System, creating up to 24 independent channels. Chan...

Direct On-Membrane MS/MS Analysis using the Chemical Printer (CHIP-1000) and AXIMA-QIT

...cale on-membrane PMF analysis can be conducted on proteins transferred to a pvdf membrane utilizing the microdispensing function of a chemical printer (Refe...om E. coli separated by 2-dimensional electrophoresis were transferred to a pvdf membrane for use as the sample (Fig. 1). A chemical printer was then used...

Direct Counting of Millipore MultiScreen Filtration Plates.

...ston, Massachusetts (Cat. No. NED- 009). We used the white MultiScreen-HV plate (Millipore Cat. No. SA2M060E2), which contains a Durapore pvdf 0.45 mm membrane. A standard competition curve was prepared, following the instructions provided with the reagents. After incubation, the ligand-recep...

Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

...brane with low background fluorescence at wavelengths suitable for the fluorophores. Both a nitrocellulose membrane (Hybond ECL) and a newly developed pvdf membrane (Hybond–LFP) were found to meet these expectations.

Detection and quantitation of a phosphoprotein ...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...omophenol blue). After being electrophoresed on 7.5% polyacrylamide gels, separated proteins were electrophoretically blotted to pvdf membranes (5.5 x 8.5 cm) at a constant current of 2.0 mA/ cm 2 of gel surface for 30 minutes. Blots were either stained with 0....

Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell

...quired for semi-dry blotting. pvdf provides superior retention of the protein. For pr...sults. It is important to first wet the pvdf in 100% methanol, then equilibrate in bottom/anode...g vertical electrophoresis: 1. Wet the pvdf membrane in 100% MEOH, then equilibrate in bottom/...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

... bromophenol blue). After being electrophoresed on 7.5% polyacrylamide gels, separated proteins were electrophoretically blotted to pvdf membranes (5.5 x 8.5 cm) at a constant current of 2.0 mA/ cm 2 of gel surface for 30 minutes. Blots were either stained with 0....
PVDF in Biological Products

Immun-Blot PVDF Sandwiches, 7 x 8.5 cm, 20 from Bio-Rad

Description: Immuno-Blot PVDF membrane and filter paper sandwiches are composed of a precut Immun-Blot PVDF membrane and two sheets of thick filter paper that are preassembled into a blotting membrane and filter paper sandwich. These 7 x 8.5 cm membrane sandwiches for high binding capacity immunoblotting fit Re...
Company:Bio-Rad

N-terminal Sequencing from Primm Biotech, Inc.

Description:... Provide us with target blotted protein on pvdf membrane or with protein in solution (preferably in water) The N-terminal ... by HPLC or mono-and bidimensional SDS-PAGE, followed by electroblotting on pvdf (Polyvinylidene difluoride) membranes using the well known EDMAN degradatio...
Company:Primm Biotech, Inc.

Human Granzyme B ELISPOT Kit from DiaPharma Group, Inc.

Description:... ELISpot kit for human Granzyme B. Kits contain pvdf plates, capture & detection antibodies, streptavidin-ALP, BSA, Skimmed dry milk, BCIP/NTB substrate, and wash buffer...
Company:DiaPharma Group, Inc.

ECL Plus Western Blotting Reagent Pack from GE Healthcare, formerly Amersham Biosciences

Description:... reagents for membrane blocking and secondary detection of membrane-bound mouse and rabbit primary antibodies. Designed for use with nitrocellulose or pvdf membranes. Detection reagents are not included, but the system is optimized for use with ECL Plus. Category: Blotting & Labeling & Detection, Protein...
Company:GE Healthcare, formerly Amersham Biosciences

Mouse Immunoglobulin Chromogenic Kit, WesternBreeze Chromogenic from Invitrogen

Description:... WesternBreeze Immunodetection Kits are designed for ultra-sensitive detection of primary antibodies immobilized on nitrocellulose or pvdf membranes. Since all necessary reagents are supplied (except membranes and primary antibodies), the WesternBreeze Kits save you time in both optimizin...
Company:Invitrogen

Chemical Inkjet Printer from Shimadzu Biotech

Description:...y systems deliver picoliter volumes of reagents to defined locations on the pvdf membrane (supports four different chemistries for multiple enzyme digests o...gestion of protein using pico to nanoliter volumes of reagents delivered to pvdf electroblots from 2-D gels Piezoelectric drop-on-demand ink-jet technology ...
Company:Shimadzu Biotech

E-PAGE Blotting Pads (Reusable) 8.6 cm x 13.5 cm from Molecular Probes (Invitrogen)

Description:...f pre-cut pre-assembled membrane/filter paper sandwiches that perfectly fit 8.6 cm x 13.5 cm E-PAGE Gels*. The available nitrocellulose and Invitrolon pvdf membranes are designed for western transfer of proteins (Figure 1 Table 1). Pre-cut extra-thick absorbent Blotting Paper (2.5 mm thickness) for E-PAGE...
Company:Molecular Probes (Invitrogen)
Other Tags
(Date:3/31/2015)... In conjunction with the Asbestos ... the Mesothelioma Applied Research Foundation (Meso Foundation) announced ... complement its existing work in mesothelioma research, education, ... the Meso Foundation’s mission, we want to eliminate ... to asbestos, including mesothelioma,” said the Meso Foundation’s ...
(Date:3/31/2015)... (PRWEB) March 31, 2015 Karen A. ... 2015 at Rivier University’s Commencement ceremony on ... of humane letters in recognition of her eminent career ... Serving as a front-line caregiver and senior staff nurse ... years and advancing to leadership roles, including President of ...
(Date:3/31/2015)... Many people, including the ones with physical ... cycling are only for able-bodied participants. , A ... aims to change that thinking. , The program, ... Adaptive Sports Expo on Saturday, April 11 (10 a.m. ... Hollywood. , The event will showcase a wide ...
(Date:3/31/2015)... (PRWEB) March 31, 2015 OnChip Devices ... introduced a wire-bondable silicon ESD diode chip targeted towards low ... diode offerings, these Zeners are ideal for protecting circuits by ... diode can handle pulses of up to 350W and ... of the IEC 61000-4-2 standard. These diodes do not ...
(Date:3/31/2015)... Phoenix, AZ (PRWEB) March 31, 2015 The ... on the GMO controversy , just kicked off their ... oft-controversial term “substantial equivalence.” , “ Substantial Equivalence was ... United States,” states Eric Battersby, Director at Change The World ... to a point well before GMOs even entered our food ...
Breaking Medicine News(10 mins):Health News:Mesothelioma Research Organization Announces New Prevention Program 2Health News:Mesothelioma Research Organization Announces New Prevention Program 3Health News:Rivier University to recognize nurse leader, educator and safety advocate at 80th Commencement on May 9th 2Health News:Rivier University to recognize nurse leader, educator and safety advocate at 80th Commencement on May 9th 3Health News:Memorial Healthcare System's Adaptive Sports Expo Encourages Physically Challenged To Go Beyond Perceived Limitations 2Health News:OnChip Introduces Low Voltage Diodes for High-Brightness LED Protection 2Health News:The Walk a Mile Project Launches New Research Sequence on Substantial Equivalence of GMOs 2
(Date:3/17/2015)... 17, 2015  MecklerMedia Corporation (OTCQX: MECK) announced ... service robots ever held in New ... May 11-13, 2015 at the Javits Convention Center. ... event include: Acorn Product Development; Axis NJ; c-Link ... Robotics; Littler; NewBotic Corporation; Neya Systems LLC; Reliabotics; ...
(Date:3/12/2015)... -- Beta Systems today announced Fast ... (IAM) solution for a fixed price and with ... IAM package, customers benefit from the 30 years, ... implementations across different industries. The new fixed-price IAM ... necessary services and consulting. It spans the implementation ...
(Date:3/10/2015)... 2015  Continuing its 167-year history of offering the ... The Eye Scanning Password Authenticator , a device ... secure websites or sensitive data. Employing the ... borders, the device has a small camera that scans ... iris, converting them into an encrypted ID that cannot ...
Breaking Biology News(10 mins):MecklerMedia's RoboUniverse New York Announces Sponsors and Exhibitors, May 11-13, 2015 2Beta Systems Fast Forward IAM Available Now: a Standardized IAM Package at a Fixed Price 2Hammacher Schlemmer Introduces The Eye Scanning Password Authenticator 2
Other Contents