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PRECOLUMN in Biological Technology

ANALYSIS OF AMINO ACIDS BY REVERSED PHASE CHROMATOGRAPHY WITH PRECOLUMN DERIVATISATION AND UV/VISIBLE DETECTION

Key words: Amino Acids, RP-HPLC, Precolumn derivatisation, o-Phthalaldehyde (OPA), 2-Mercaptopropionic acid, Ultraviolet detection. Introduction: Underivatised amino acids do not significantly absorb light in the UV/visible spectrum. In order to increase...
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