Sangamo BioSciences Presents Positive Phase 2 ZFP Therapeutic Data at ADA 2009

...oints in previous pivotal large scale trials of other non-analgesic approaches for diabetic neuropathy." About SB-509 SB-509 is an injectable plasmid encoding a DNA-binding Zinc Finger DNA-binding Protein (ZFP) Transcription Factor (ZFP TF(TM)) designed to upregulate the endogenous expression of th...

Five UCSF Scientists Elected to National Academy of Sciences

... tolerance-inducing cells in the thymus that express the beta-cell specific insulin gene. As a graduate student, Hanahan developed high efficiency plasmid transformation methods and E. coli strains that have facilitated DNA cloning procedures; his work on E. coli transformation (the production of "c...

iZumi Bio and Kyoto University Form Collaboration to Advance Induced Pluripotent Stem Cell Technology

...ctober 2008, Dr. Yamanaka's group reported for the first time a virus-free method for inducing pluripotency in differentiated cells by using a pair of plasmid vectors to introduce the four iPS genes into embryonic mouse fibroblasts as part of CiRA's efforts to establish safe and efficient methods to generate...

Senesco Announces Results of Pre-Clinical Efficacy, Toxicology and Dose Ranging Studies for Multiple Myeloma Therapeutic Candidate

... About SNS-01 Senesco's SNS-01 consists of three components: an eIF5A plasmid, an siRNA against eIF5A and polyethyleneiminie (PEI). The eIF5A plasmid upregulates the apoptotic pathways within cancer cells. The siRNA downregulates proinflammatory cytokines, NF-kB and ICAM, which are proliferation fa...

Rockefeller University's Center for Clinical and Translational Science funds 18 new pilot studies

...se to a novel vaccine against pancreatic cancer. Investigators will administer a monoclonal antibody, which targets dendritic cells, followed by a DNA plasmid that works as a booster. Ype de Jong, M.D., Ph.D., visiting fellow, Charles M. Rice's Laboratory of Virology and Infectious Disease, to define the...

Therapeutic DNA Vaccine Company Inovio Biomedical Reports Second Quarter 2008 Financial Results

...ients' T-cell responses to the hepatitis C virus was recorded in conjunction with the viral load reductions. Pre-clinical results from two proprietary plasmid DNA-based universal influenza vaccine candidates using the company's proprietary electroporation delivery technology and, specifically, a new intrader...

Plasmid in Medical Technology

Senesco Chooses VGXI to Produce Factor 5A Plasmid

NEW BRUNSWICK, N.J., July 3 /PRNewswire-FirstCall/ -- Senesco Technologies, Inc. ("Senesco" or the "Company") (Amex: SNT ) announced today that it has contracted with VGXI, Inc. (The Woodlands, TX) ("VGXI") to supply the plasmid portion of the Company's combination therapy consisting of the F...

Vical Pandemic Influenza Vaccines Achieve T-Cell Responses and Cross-Clade Reactivity in Humans

... responses through Day 182. Vical's monovalent vaccine contained a plasmid (a closed loop of DNA) encoding the hemagglutinin (HA) surface protein from...ell responses against H5 as well. Vical's trivalent vaccine contains the H5 plasmid plus separate plasmids encoding consensus sequences of two highly conserved...

Vical Receives Milestone Payment from Merck & Co., Inc. Triggered by Planned Initiation of Investigational Cancer DNA Vaccine Trial

...t of a $1.0 million milestone payment from Merck & Co., Inc. based on Merck's planned initiation of a Phase I clinical trial of an investigational plasmid DNA (pDNA) cancer vaccine. The candidate vaccine is based on Vical's DNA gene delivery technology and encodes human telomerase reverse transcriptase (...

Karolinska Institutet to Test Vical's Vaxfectin(R) Adjuvant With Preventive Vaccine against HIV Under Sponsorship of Swedish Institute for Infectious Disease Control

...times. The two vaccine components differ in how the genes are packaged. The DNA prime vaccine component contains only the specific gene sequences in a plasmid DNA ring, and cannot reconstitute into an infectious virus. The HIV DNA vaccine was developed by the SMI and the Karolinska Institutet in Sweden. The ...

Vical Announces Breakthrough for Pandemic Influenza DNA Vaccines With Preliminary Human Data

... faster development and production. Vical's monovalent vaccine contains a plasmid (a closed loop of DNA) encoding the hemagglutinin (HA) surface protein from...ell responses against H5 as well. Vical's trivalent vaccine contains the H5 plasmid plus separate plasmids encoding consensus sequences of two highly conserved...

Vical Advances RapidResponse(TM) DNA Vaccine Platform Under $6 Million Grant from NIH

...st effective dose, head-to-head comparison against plasmid DNA vaccine, Vaxfectin(R) formulation optimization...overage includes delivery of linear DNA as well as plasmid DNA. Currently plasmid DNA vaccines, which are under development against ...

Plasmid in Medical Products

E-Gel®96 High-Throughput Agarose Electrophoresis System

Description:... into an automated, high-throughput operation with the E-Gel 96 system. This bufferless, pre-cast system is ideal for analyzing multiple PCR products, plasmid preparations, restriction digests, and more. Fully automated, robot-compatible, and ready to put into action, the E-Gel 96 system makes your high-thro...
Company:Invitrogen

hGH ELISA

Description:...he hGH ELISA is used to quantitatively measure the expression of hGH released into the cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene encoding for hGH. hGH Elisa is standardized, highly sensitive, specific, fast, no leaching, comparable, easy to perform and...
Company:Roche Diagnostics

QIAvac 24 Plus

Description:...oading of spin columns into and out of a centrifuge. The new QIAvac 24 Plus offers: Time savings rapid vacuum-driven purification and cleanup of plasmid or genomic DNA, PCR products, and RNA Convenience minimal hands-on-time Flexibility process up to 24 columns in parallel Versatility su...
Company:QIAGEN

Threshold Total System (110V)

Description:... (e.g., BSA, IgG, insulin, transferrin), Proteins A and G, and any unique protein that can be bound by antibodies as well as sequence-specific DNA and plasmid copy number. The system reduces the time required to develop, validate, and perform ultra-sensitive assays with maximum speed, sensitivity, reproducib...
Company:Molecular Devices Corporation

Terrific Broth, Ready-Made Powder

Description:...Phosphate Monobasic: 4% Storage: Ambient To Use: Dissolve 47 gm powder per liter of purified water. Autoclave. Application: Provides greater yields of plasmid DNA and recombinant proteins than LB media by allowing for greater cell density....
Company:USB Corporation

VersaFluor Cuvette Fluorometer

Features:... Changeable filters Is the only instrument needed to detect and quantitate DNA from PCR amplification, restriction digests, and plasmid preps, to quantitate apoptosis activity, and to assay the reporter enzymes beta-glucuronidase (GUS) and beta-galactosidase Quantitates DNA at concen...
Company:Bio-Rad Laboratories

Plasmid in Biological News

Researchers observe single protein dimers wavering between two symmetrically opposed structures

...searchers examined a protein known as Rop (repressor of primer), expressed in E. coli bacteria, which binds to RNA to regulate the number of copies of plasmid genes produced within the bacteria. This small "homodimer" is made up of two identical hairpin coils of amino acids, and has often been used as a mode...

Another JDRF partner moves research forward with collaboration agreement for diabetes treatment

...agreement for a beta cell regenerative therapy with Lilly. Bayhill's BHT-3021 compound is now in Phase I/II clinical trials supported by JDRF, is a plasmid encoding proinsulin designed to target specific pathogenic immune cells involved in the immune response of type 1 diabetes. ...

Evolutionary origin of bacterial chromosomes revealed

...Pacific University, remarked: "The findings in this work provide substantial new evidence that second chromosomes in all bacteria studied to date have plasmid origins. While other mechanisms leading to second chromosome formation seem possible, it is intriguing that this approach has been most productive." ...

New insights into a leading poultry disease and its risks to human health

...izing animals and causing disease. Over time, each plasmid becomes a patchwork quilt of DNA information, cont... database. Among the disease causing parts of the plasmid pAPEC-1 are a series of genes that make up protein...cipient bacteria. By analyzing the DNA sequence of plasmid pAPEC-1 and testing the mechanism of transfer of p...

Bayhill Therapeutics and the Juvenile Diabetes Research Foundation announce research collaboration

...patients require the life-long administration of insulin, which is the only approved therapy for type 1 diabetes. About BHT-3021 BHT-3021 is a plasmid encoding proinsulin designed to tolerize the immune system, thereby turning off the self directed immune attack against the islet cells. This product ...

Yamanaka eliminates viral vector in stem cell reprogramming

... the injected cells contributing to almost all cell types. Still, other problems remain to be solved. The efficiency of the gene transfer with the plasmid was lower than with the retrovirus. Nevertheless, this significant step moves us closer to realizing the promise of stem cells in the understanding an...

Plasmid in Biological Technology

High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

James Batchelor, Lynn Jordan, and Ed Alderman Applied Science and Technology Department, Caliper Life Sciences, Inc., Hopkinton, MA 01748 Karen Heins and Olaf Stelling Agencourt Bioscience Corporation, 100 Cummings Center, Suite 107J, Beverly, MA 01915 ...

Aurum Plasmid 96 Kit Tech Note, Rev A

Neil Duldulao, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 USA Introduction E. coli bacteria have long been used as hosts for plasmid propagation. Several methods have been used for preparing the plasmid, including ...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

Audra Day, Colette Schneider, Lisa Ottmers, and Brandt L. Schneider Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. Introduction Small-scale isolation and purification of plasmi...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano and H o w a rd J. Federo ff, University of Rochester, Rochester, NY, USA Introduction Transfection efficiency is dependent upon a number of factors, including method of transfection...

SPRINTPREP SINGLE STEP PLASMID PURIFICATION

SprintPrep is an efficient SPRI-based plasmid purification system that can be completed in less than 15 minutes. Unlike most traditional plasmid purification chemistries, SprintPrep isolates plasmid DNA directly from overnight cu...

COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION

CosMCPrep is a versatile SPRI-based, plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated and produces high quality templ...

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Plasmid in Biological Products

pPDM-1 Cloning Plasmid from EPICENTRE Biotechnologies

Description: Request Info...
Company:EPICENTRE Biotechnologies

PureLink HiPure Plasmid Filter Midiprep Kit from Invitrogen

Description: From micro to giga sizes, from single prep to high-throughput, Invitrogen's range of plasmid purification kits offers superior performance to match the demands of your downstream application....
Company:Invitrogen

pKD-NegCon-v1 Human siRNA expression plasmid from Upstate

Description: Negative Control, eluted and packaged aseptically in 20l of 10mM Tris-HCL, pH 8.0, 1 mM EDTA, 5 years at either 4°C or -20°C...
Company:Upstate

SuperScript Plasmid System and MAX Efficiency DH10BCompetent Cells from Invitrogen

Description: ...
Company:Invitrogen

cDNA Library Construction Services from Bio S&T

Description:... In plasmid or lambda vector. Please inquire for more details!...
Company:Bio S&T

Gtporator (4 ml) from GEN-TREND s.r.o.

Description:... Highly efficient electroporation solution suitable for transfection of plasmid DNA, oligonucleotides and siRNA. Compatible with all common electroporators....
Company:GEN-TREND s.r.o.

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Plasmid in Biological Definition

Plasmid

Figure 1 : Schematic drawing of a bacterium with plasmids enclosed. (1) Chromosomal DNA . (2) Plasmids Plasmids are (typically) circular double-stranded DNA molecules that are separate from the chromosomal DNA (Fig. 1). They usually occur in bacteria , sometimes in eukaryotic ...

Antibiotic resistance

...tic resistance is the ability of a microorganism to withstand the effects of an antibiotic . Antibiotic resistance develops through mutation or plasmid exchange between bacteria of the same species . If a bacterium carries several resistance genes, it is called multiresistant or, informally, a ...

Bacterial conjugation

...njugation, one of the bacteria has to carry an F- plasmid , the other one must not. The F-plasmid (also called F-factor) is an episome (a plasmid that can integrate itself into the bacterial chro... bacterium (5'-end first). The now single-stranded plasmid is filled up with a complementary strand by a roll...

Cytoskeleton

...e plasmids encode a partitioning system that involves an actin-like protein ParM . Filaments of ParM exhibit dynamic instability , and may partition plasmid DNA into the dividing daughter cells by a mechanism analogous to that used by microtubules during eukaryotic mitosis . Crescentin The bacter...

DNA

...long segments difficult to separate. Circular DNA When the ends of a piece of double-helical DNA are joined so that it forms a circle, as in plasmid DNA, the strands are topologically knotted. This means they cannot be separated by gentle heating or by any process that does not involve breaking ...

Hfr cell

... A hfr cell (also called hfr strain ) is a bacterium with a conjugative plasmid (often F ) integrated into its genomic DNA. Hfr is the abbreviation for high frequency recombination . Unlike a normal F+ cell, hfr strains will,...

Plasmid in Biological Dictionary

Plasmid

A plasmid is an extra-chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently of the chromosomal DNA. In many cases, it is circular and double-stranded. Plasmids usually occur... The sequence starting with which the plasmid -DNA will be r...

Transfection

..., electroporation) ... Transfection reagents are highly efficient for plasmid DNA and siRNA ... Transfection is the transient or stable introduction o...nd a range of specialized transfection reagents that effectively deliver plasmid DNA, siRNA or duplex RNAi, oligonucleotides, and RNA into eukaryotic cell...

Sub-cloning

...es,in-frame deletions and ... Allele Biotechnology and Pharmaceuticals, Inc. is a San Diego based company with a focus on ... Sub Cloning & plasmid Construction. Vector Construction ... An ANSI-C program finding sub - cloning strategies, in-frame deletions and ... quickly find sub - cloning ...

Subcloning

...a subcloning experiment is in principle quite simple. ... tive to CIAP for dephosphorylating DNA for subcloning exper- iments. ... Subcloning plasmid DNA Constructs. By Bob McLaren, Ph.D. Promega Corporation ... Vectors used for genomic, cDNA, and subcloning . General definition. Cloning Vector ...

Simple Sequence Repeat

...ple Sequence Repeat ... SSRHunter 1.3 Simple Sequence Repeat Search tool ... Sequence Format Conversion Utilities. Phylogenetic Analysis. plasmid ... ...

Shuttle vector

... do not integrate into the genome and do not rearrange. ... We used the plasmid pRN1 as starting point to construct shuttle vectors for Sulfolobus. .....at least (a) a region ... Its use in Shuttle Vector Construction, 1989, plasmid 22: 193-202. ... Provided are replication competent, recombinant ade...