PCRs

Pwo Master*: Simplify Setup of Your Proofreading PCRs

With the introduction of the Pwo Master, Roche Applied Science now offers a new, ready-to-use proofreading master mix for the robust amplification of fragments up to 3 kb, providing the highest possible fidelity during PC...

Reduce Errors and Save Time When Setting Up PCRs

Polymerase chain reaction (PCR) setup typically requires thawing of the various reagents, setting up a master mix, then distributing the mix to the individual reaction tubes. The various handling steps ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...he cDNA. All primers (Table II) are intron spanning to produce a cDNA specific PCR product. To avoid false positive results by PCR product carry-over, pcrs are setup in a flow hood. 17. 15 l of each PCR product were loaded on a 2% agarose gel and separate...

The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B

... coagulation factor V gene (van den Bergh et al. 2000). Duplicate pcrs were carried out in a volume of 50 l containing 20 mM Tris, pH 8... from the optimization experiment are shown in Figure 3. Duplicate pcrs containing genomic DNA from a WT individual, genomic DNA from a ...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

...s were obtained for the β-actin mRNA amplification assay. Quantitation of GAD67 Gene Expression The pcrs were quantitated by selecting the amplification cycle where the PCR product of interest was first detected (C T )). To account for differen...

GENE SILENCING WITH PCR PRODUCTS

... the siRNA sequence are designed and ordered; (II) the oligonucleotide(s) are used as primers in one or more pcrs with the included RNA Pol III promoter-containing DNA template; and (III) the resulting PCR product is colum...

Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System

...that delivers high yield and great fidelity for amplification of fragments up to 5 kb. Expand the Performance of pcrs up to 5 kb Expand HiFiPLUS Enzyme Blend successfully challenged four products from different suppliers, and prov...

PCR-Grade Nucleotides Larger Pack Sizes Now Available

...s (dATP, dCTP, dGTP, and dTTP) in separate vials, is now available in a 4 x 125-mol pack size. This larger pack size is sufficient for 6,250 100-l pcrs at a final dNTP concentration of 0.2 mM. The 500-mol pack size of single dNTPs provides 25,000 reactions. Dide...

Roche Diagnostics Corporation

... Challenge the Performance of Your Hot-Start pcrs with FastStart Taq DNA Polymerase and the Novel Fa... Reduce Errors and Save Time When Setting Up pcrs Proteinase K, Recombinant... Pwo Master*: Simplify Setup of Your Proofreading pcrs The DIG System Nonradioactive Automated H...

Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System

...t Taq DNA Polymerase has proven to be: The most robust enzyme preparation currently available Even the most challenging pcrs are no longer a problem. In applications where other polymerases stop working, FastStart Taq DNA Polymerase gives effective results (Figures 1, ...

PCRs in Biological Products

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Kidney from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Peripheral blood leukocyte from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Spleen from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Colon from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Lung from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Human Brain (fetal) from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Rat Brain (adult) from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Thymus from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (19 - day) from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies

Rapid-Screen Arrayed cDNA Library Panels Master Plates -Mouse Embryo (12.5 - day) from OriGene Technologies

Description:... The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of pcrs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well "Master Plate," where each well contains plasmid DNA from 5,000 c...
Company:OriGene Technologies