Cell microenvironments hold key to future stem cell therapies
... actual in vivo conditions and enables us to perform highly parallel functional analysis of combinatorial microenvironments, and image analysis of 3-D organotypic
cultures and micro patterned culture substrata," said LaBarge. "The 3-D capability is crucial because our studies show that orientation of the stem or...
Genetic breakdown in Fanconi anemia may have link to HPV-associated cancer
...utations in the FANCA gene.
The HPV cancer genes E6/E7 were artificially introduced to FANCA-patient skin cells and grown in the three-dimensional organotypic
raft culture that simulates skin. The investigators observed irregular cell growth and increased skin thickness in the culture. Restoring FANCA gene f...
Organotypic in Medical Technology
Organotypic in Biological News
Time (and PPAR-beta/delta) heals all wounds
...t could communicate with each other," says Tan. "Organotypic skin cultures are a really good technique for this."
First developed in the 1980s (3), organotypic
skin cultures (OTCs) are made by embedding dermal fibroblasts in a gel of extracellular matrix proteins. Keratinocytes are seeded on top of this gel a...
Cells united against cancer
...und and suppresses oncogene
function. "We were amazed to find out that the formation of organized
mammary epithelial architecture in three-dimensional organotypic
culture correlated with complete loss of oncogenic activities of c-Myc
cancer gene" says Klefstrom.
Johanna Partanen, a graduate student in Kl...
Organotypic in Biological Technology
Biolistic Transfection of Organotypic Brain Slices
A. Kimberley McAllister, Nina Tang, Lawrence C. Katz, and Donald C.
Department of Neurobiology, Box 3209, Duke University Medical Center,
Durham, NC 27710
The introduction of genetic material ...