Found: Missing sequence of the human Y chromosome
Scientists report today in the journal GenomeResearch that they have successfully cloned and characterized apreviously intractable DNA sequence: a 554-kilobase-pair genomicsegment near the centromere of the human Y chromosome. This sequencecontains eight putatively active genes that could be implicated insex-associated height differences and gonadal tumor development. Thispericentrom...Needling Chromosomes Reveals Cell Division Secret
By impaling individual chromosomes with glassneedles one thousandth the diameter of a human hair, a Duke Universitygraduate student has tested their "stickiness" to one another duringcell division. Her uncanny surgical skills have added a piece to thelarge and intricate puzzle of how one cell divides into two -- aprocess fundamental to all organisms.In the Dec. 14, 2004, issue of Current Bi...Variation in women's X chromosomes may explain differences among individuals, between sexes
The first comprehensive survey of gene activity in the X chromosomes of women has revealed an unexpected level of variation among individuals, according to new work by researchers at the Duke University Institute for Genome Sciences & Policy (IGSP) and Pennsylvania State University. The results may have important implications for understanding the differences in traits among women and...Liposome finding implies electrical effect on cell development
Experiments with liposomes ?cell-like "water balloons" composed of artificially created phospholipid bilayers similar to natural cell membranes ?have revealed unexpected behavior in the presence of electrical fields that may provide a paradigm-shifting change in science's understanding of biomembrane function in operating living systems. Arizona State University chemists Mark Hayes and Mic...Chromosome Deletion Predicts Aggressive Neuroblastoma
When genes are deleted on a particular section of chromosome 11, the result is an aggressive form of the childhood cancer neuroblastoma. A new study suggests that detecting this genetic deletion during the initial evaluation of children with neuroblastoma may indicate to physicians that they should recommend a more aggressive regimen of chemotherapy to fight the cancer. Edward F. Attiyeh,...Canine Pancreatic Microsomal Membranes from Promega
Description:Microsomal vesicles are used to study co-translational and initial post-translational processing of proteins. Processing events such as signal peptide cleavage, membrane insertion, translocation and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes. In addition, processing and glycosylation events may be studied by...Nucleosome ELISA Kit from Calbiochem
Description:PROTECT FROM MOISTURE. AVOID FREEZE/THAW CYCLES. This kit allows the quantitation of apoptotic cells by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. In this assay, mono- and oligonucleosomes are captured on pre-coated DNA binding proteins. Anti-Histone 3 (H3) biotin-labeled antibody then binds to the histone component of c...Chromosomal Grade Agarose from Bio-Rad
Description:Chromosomal Grade Agarose was developed specifically for pulsed field separations of DNA larger than 2 megabase pairs. This agarose has the highest gel strength of any electrophoresis agarose available, allowing preparation of very low percentage agarose gels. Megabase DNA separations done in Chromosomal Grade...Chromosomal Grade Agarose from Bio-Rad
Description:...Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
Cell death as a counterpart to cell proliferation plays anessential role in the homeostasis of cell numbers in adultorganisms, furthermore in eliminating cells damaged byirradiation, chemotherapeutic drugs, hyperthermia andother influences [1-3]. There are two distinct...PFGE Analysis of Chromosomal DNA From a Single Hair Root
Contributed by Jacqueline Boultwood, Gail M. Abrahamson, and J.S. Wainscoat, Leukemia Research Fund, Molecular and CytogeneticHaematology Unit, John Radcliffe Hospital, Headington, OxfordOX3 9DU, United Kingdom Tumor diagnoses, DNA fingerprinting, and other genetic analysisoften require DNA obtained in a no...Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
Volker Wippersteg, Katja Kapp, Werner Kunz, and Christoph G Grevelding,Institute for Genetics, Center of Biological and Medical Research,Heinrich-Heine-University, D-40225 Dsseldorf, Germany Schistosomes are parasitic helminths that cause bilharzia (schistosomiasis) in humans, a tropical disease of worl...Electroporation of Yeast Artificial Chromosomes
Maren Bell and Robert Mortimer, Human Genome Center, Divisionof Cell and Molecular Biology, Lawrence Berkeley Laboratory,Berkeley, California 94720 Transformation of yeast with yeast artificial chromosomes (YACs) has traditionally been performed by a PEG-spheroplast procedure. However, the...Analysis of a Verapamil Microsomal Incubation using Metabolite ID and Mass FrontierTM
The characterization of a drugs metabolites plays a crucial role throughout its development. In the early discovery p...Chromosome Ends Trigger DNA Damage Response For Telomere Protection
Researchers from the Salk Institute for Biological Studies have suggested that a localized DNA damage response at telomeres after replication is essential for recruiting the //processing machinery that promotes formation of a chromosome end protection complex. Telomeres, the ends of linear chromosomes, represent a problem for the control of genome stability and the DNA damage machinery...Chromosomes to predict oral cancer
The DNA content in cells of oral leukoplakia, specifically the number of chromosomes, can be used to predict the risk of oral carcinoma, according to study.// 123 patients were taken up for research for an average of 8.5 years, found that oral leukoplakia with the normal number of chromosomes — 46 — were unlikely to develop into cancer. Of the 103 patients with leukoplakia with normal DNA...Gestational Diabetes linked to Chromosomal defici
According to a new study, researchers, suggest that, some women who develop gestational diabetes during pregnancy may be prone to have children with certain chromosomal abnormalities However, researchers stress, gestational diabetes is not itself a risk factor for these types of defects since they form in the early stages of pregnancy--well before gestational diabetes typically develops./...Y chromosome uses trick to repair its own genes
Scientists have discovered that the Y chromosome uses a neat trick to repair its most crucial genes, a strategy that apparently helps keep it from rotting away over evolutionary time. The Y chromosome, of course, is best known for supplying the biological signal that makes males rather than females. Some scientists have suggested it might disappear millions of years from now becaus...Indian Scientists Make Wide-Ranging Analysis And Annotation Of X Chromosome
Researchers at Institute of Bioinformatics (Bangalore) had made a study of the genomic sequence of human X chromosome// with its transcriptome and proteome sequences as well. The IOB analysis showed that the human X chromosome has a gene density less than other chromosomes with 43 novel protein-coding regions and 696 known genes. The researchers have been able to identify and find ou...