Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

... automated nanoflow LC/MS/MS system, configured with a PepFinder Kit (Figure 1). Aliquots of 10 μL were placed in wells of a 96-well plate (Nalge nunc International, Rochester, NY, USA). The plate was sealed with plastic film to minimize evaporation and inserted into the autosampler of a Finnigan Sur...

Precision Testing for the Sciclone Advanced Liquid Handler Z8 Option

...erformed using the deck layout (Fig.1) and a protocol to execute the following: Deliver 100μL of water into each well of a nunc (Denmark) 96 well, clear bottom, untreated plate using the Bulk Dispenser. Attach 100μL Automation Certified tips to the ...

Sciclone ALH Bulk Dispense Precision Testing

...on Tartrazine volumes (10, 20, 40 or 80μL) were dispensed twice into a waste reservoir then secondary dispensed into each column of a nunc (Denmark) 96 well, clear bottom, untreated plate with the Bulk Dispenser (Fig.1). Deionized/degassed water was then dispensed into each well to provid...

Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)

... 2. High-quality microplates; e.g.: For dispense volumes >150 L Greiner flat bottom plates (E & K Products, catalog #565101) nunc flat bottom plates (Fisher Scientific, catalog #12565226) For dispense volumes 40 L - 150 L Costar half area EIA/RIA plates (Costar, catalo...

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

...were obtained fromResearch Genetics, Huntsville, Alabama. The oligonucleotide sequences are notimportant to this discussion. The microplates used were nunc brand, black 384well microplates, surface-treated to minimize binding (cell culture-treated). Allsamples were prepared in 10 mM Tris buffer, pH 8, con...