Work with fungus uncovering keys to DNA methylation
...quickly reproducing and easy-to-manipulate fungus, neurospora crassa, to explore the control of DNA methylation. neurospora is considered the simplest model organism for such...m. Our assumption is that a lot of what we find in neurospora will be applicable to other systems." In this c...Two new studies on circadian rhythms
...ey build on the research of team leaders, Drs. Jay Dunlap and Jennifer Loros, who have documented the workings of FRQ and most other components in the neurospora clock. "The Cell paper describes how the cell uses phosphorylation of a clock protein to keep the period length of the cycle close to the same acr...Study shows more genes are controlled by biological clocks
...lity of Computing Life came from studying genes in neurospora crassabread mold. In fact, much of what science kn...y 16 clock-controlled genes had been discovered in neurospora in more than 40 years of research. Arnold's team u.... The UGA scientists discovered how three genes in neurospora make such a clock tick at the molecular level. ...Novel connection found between biological clock and cancer
...age is fixed. The researchers found that the resetting effect of DNA damage requires the period-4 clock protein, and that period-4 is the homolog, the neurospora version, of the mammalian checkpoint kinase. Moreover, the clock regulates expression of the period-4 gene. This closes a loop connecting the clock...Efficient Transfection of Neurospora Crassa
Katherine A. Borkovich PhD and Kimberly Vollmer Katherine A. Borkovich PhD, University of Texas-Houston Medical Center, Dept. of Microbiology and Molecular Genetics, Houston, Texas. Kimberly Vollmer, Brinkmann ...Efficient Transfection of Neurospora Crassa
Katherine A. Borkovich PhD and Kimberly Vollmer Katherine A. Borkovich PhD, University of Texas-Houston Medical Center, Dept. of Microbiology and Molecular Genetics, Houston, Texas. Kimberly Vollmer, Brinkmann ...Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells
... Cryptococcus neoformans, ma5 mutants Dictyostelium discoideum Fusarium solani Leptosphaeria maculans neurospora crassa Pichia pastoris Penicillium urticae Saccharomyces cerevisiae (6 strains) Schizosaccharomyces ...Transformation of Filamentous Fungi by Microprojectile Bombardment
... bombardment. Plasmid pRG-1, a 5 kb plasmid containing the neurospora crassa pyr4 gene cloned into pUC9 was used for transformation ex... of Aspergillus nidulans by the ornithine-5-phosphate decarboxylase gene of neurospora crassa, Biochem. Biophys. Res. Comm., 112 , 284-289 (1983). ...Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit
... pLorist6Xh [3] was used in this experiment for screening of the neurospora crassa genome. Restriction analysis of the pLorist6Xh vector iso...aw Hill, New York, 2000. Kelkar, H.S., et.al., The neurospora Crassa Genome: Cosmid Libraries Sorted by Chromosome, Genetics...