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NaCl in Medical News

Emily's Law for Regulating Pharmacy Technicians Signed by Ohio Governor

...line base solution, as opposed to using a commercially manufactured (prepacked) IV solution bag. Standard IV bags contain a base solution of 0.9% nacl (sodium chloride); the base solution prepared for Emily contained approximately 20x the standard concentration of sodium chloride. Prior to entering a...
NaCl in Medical Products

LB-BROTH, liquid, 10 g NaCl

Description: Luria bertani broth: 1.0% Tryptone, 0.5% Yeast Extract, 1.0% Sodium Chloride....
Company:BioSource International

L-BROTH, liquid, 5 g NaCl

Description: Luria bertani broth: 1.0% Tryptone, 0.5% Yeast Extract, 1.0% Sodium Chloride....
Company:BioSource International
NaCl in Medical Dictionary

Renin

...at regulates blood pressure and water (fluid) balance. Renin , which is primarily released by the kidneys, stimulates the formation of ... When nacl is elevated in the tubular fluid, renin release is inhibited. ... Renin is an enzyme made by special cells in the kidneys. ... A renin ass...
NaCl in Biological Technology

'Two-Faced' Particles Act Like Tiny Submarines

...ations of the motion of Janus microparticles with one dielectric and one metal-coated hemisphere induced by uniform fields of frequency 100Hz-10kHz in nacl solutions. The motion is perpendicular to the field axis and persists after particles are attracted to a glass wall. This phenomenon may find applicat...

Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column

...uffer consisted of 0.1M NaH 2 PO 4 (pH 6.0) to which was added 15% acetonitrile and 0.2M NaCl. In the redesigned buffer system, nacl was replaced by NaClO 4 and the phosphate buffer concentration was reduced to 0.05M. Initially, the concentration of sodium per...

HEK293 Calcium Flux Assay

...: BASE BUFFER 10 mM HEPES, pH 7.4 130 mM nacl 5 mM KCl 10 mM Glucose WASH BUFFER * 1X ... PLATE/TROUGH BUFFER * 1X Base Buffer 40 mM nacl 2 mM CaCl 2 1 mM MgCl 2 0.1% Pluronic...

Evaluation of FlashPlate in a Helicase Assay

...ed to each well of the SMP103 FlashPlate in phosphate buffer containing 1 M nacl (pH 7.4) and incubated for a minimum of 4 hours at room temperature or, alt...t room temperature, and once with 200 μl of 50 mM Tris (pH 7.5), 50 mM nacl at 37C. Each well contained approximately 10,000 to 12,000 CPM. ...

Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

...O 3 27.5 mM NaHCO 3 0.9% nacl add water 90 ml 2. Add anti-TSH into co...50 mM Tris = 0.606 g Tris (Sigma) 0.9% nacl = 0.9 g conc HCl 0.30 mL 7... Tris-HCl 0.005 M (5 mM), pH 7.5 0.9% nacl 0.005% Tween-20 5. Decant e...

Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor

...bed by Watson, et al ., 1996. In brief, 108 cells were pre-incubated at 30C for 30 minutes in HEPES [20 mM], pH 7.4, in the presence of MgCl2 [3 mM], nacl [100 mM], ascorbate [0.2 mM] and GDP [10 mM] with or without test drugs. The reaction was started by adding 10 μl of [ 35 S]-GTPγS [100 pM]....

Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer

...bility Long term stability was evaluated by performing a calibration on a multi-element standard and a blank, both being made up in 1% w/v nacl solution. The standard was then measured as a sample continuously for a period of an hour. After an hour, the reproducibility ranged between 0.8 to 2 ...

Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

...white 384-well microplate (Corning, 3705)

Buffer: 50-mM Tris pH 7.4 5-mM MgCl 2 50-mM nacl

GraphPad Prism™ software v4.0 (GraphPad Software)

Protocol Human recombinant α 2c receptor...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

...les) was used to immobilize the DNA onto the UltraGAPS slides. Coupling of DNA to CodeLink slides was achieved by incubating the slides in a saturated nacl chamber for 48 h. Refer to the CodeLink user guide (GE Healthcare) for further processing of the CodeLink slides. Conclusions ...

Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

...hemical, St. Louis, MO.) was added to the samples followed by a 30 minute incubation at 37 C. Next, 10 ml of lysis buffer (0.1 M nacl and 0.5 M Tris-HCl) was added and samples were subjected to three cycles of rapid freezing and thawing, in a -70 C ethanol bath and ...
NaCl in Biological Products

jetPEI-RGD + NaCl sol. from Polyplus Transfection

Description: Epithelial and endothelial cells-directed transfection reagent : jetPEI-RGD is a RGD peptide-conjugated polyethylenimine which allows targeted efficient transfection of integrin-expressing epithelial and endothelial cells. Integrins are adhesion molecules involved in cell-cell and cell-matrix adhes...
Company:Polyplus Transfection

Mouse Anti-Human Clusterin alpha chain Monoclonal Antibody, Unconjugated, Clone 41D from Upstate

Description:... clone 41D GenBank Accession Number : NM_203339 Immunogen : Native clusterin purified from human serum Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M nacl and 0.05% sodium azide Quality Assurance: routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates. Recognizes clusterin α chai...
Company:Upstate

Mouse Anti-Na+ / K+ ATPase alpha-1 Monoclonal Antibody, Unconjugated, Clone C464.6 from Upstate

Description:... Immunogen : Purified Na + /K + ATPase isolated from membrane fractions of rabbit kidney outer medulla Formulation: 0.1M Tris-glycine, pH 7.4, 0.15M nacl containing 0.05% sodium azide Quality Assurance: routinely evaluated in immunoblot on rat brain microsomal preparations Recognizes α-1 subunit i...
Company:Upstate

Mouse Anti-PSD-95, PDZ Domain Monoclonal Antibody, Unconjugated, Clone K28/86.2 from Upstate

Description:...n GenBank Accession Number : NM_007864 Immunogen : GST fusion to residues 77-299 of human-PSD-95 (PDZ Domain) Formulation: 0.01M Tris pH 7.4, 0.075M nacl containing 0.05% sodium azide Quality Assurance: routinely evaluated by immunoblot on rat brain microsomal protein preparation Recognizes PDZ domains ...
Company:Upstate

beta-Agarase from USB Corp.

Description:...h chloroform:isoamyl alcohol (24:1). *Add 2 volumes of TE (pH 7.6) to prevent precipitation of the oligosaccharides. Precipitate with 0.05 volumes of nacl and 2 volumes of 95% ethanol. Incubate at -20C for 30 min, then spin in a microcentrifuge. Wash the pellet twice with 70% ethanol. *The DNA solution ...
Company:USB Corp.

Ligase, T4 DNA from USB Corp.

Description:...blunt-ended DNA should be carried out at a higher concentration of both vector and insert DNA. To stimulate the blunt-end ligation reaction, 150200mM nacl and 5% PEG can be added. References: *ROSSI, R., MONTECUCCO, A., CIARROCHI, G. AND BIAMONTI, G. (1997) Nucl. Acids Res. 25 (11), 2106-2113. *WEISS, B...
Company:USB Corp.

26S Proteasome Fraction Extract from GeneTex

Description:...ce highly purified 26S is costly and more labile. Type: Proteins and peptides Form: 0.5 mg/ml in 50 mM HEPES, pH 7.6, 1 mM Mg-ATP, 150 mM nacl and 10% glycerol. ...
Company:GeneTex
NaCl in Biological Dictionary

DNA ligase

...ormation of a phosphodiester ... T4 DNA Ligase is strongly inhibited by nacl ... T4 DNA ligase overnight at 6°C. Less than 2% of ... The T4 DNA ...er bond between ... Inhibitors: T4 DNA Ligase is strongly inhibited by nacl or KCl if the ... What is DNA ligase ? Meaning of DNA ligase medi...
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