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Mutagenesis in Medical News

JCI table of contents: March 23, 2009

...ers, at San Raffaele-Telethon Institute for Gene Therapy, Italy, has now provided evidence that LVs are substantially less likely to cause insertional mutagenesis and tumors than gamma-RVs. Further, they found that modifying an element (known as the LTR) of both LVs and gamma-RVs such that it is self-inactivatin...

Evolution of human genome's 'guardian' gives people unique protections from DNA damage

...sponse elements in 14 vertebrate and non-vertebrate species. The study included researchers from the National Institute for Cancer Research (Molecular mutagenesis Unit, Department of Translational Oncology) in Genoa, Italy, and the Laboratory of Molecular Genetics Chromosome Stability Group of the National Insti...

Marco Romano, MD Joins Chiltern as Medical Director, Europe

...versity of Genova and a post-graduate degree in Clinical Pharmacology from the University of Milan. Dr Romano co-authored several scientific papers on mutagenesis and cancerogenesis, pharmacokinetics and pharmacodynamics of chemical compounds and glutathione metabolism. Dr. Romano will be located in the company...

Cigarette Smoke Alters DNA in Sperm, Genetic Damage Could Pass to Offspring

...ist as irreversible changes in the genetic composition of off-spring.' said Carole Yauk, Ph.D., lead author of the study and research scientist in the mutagenesis Section of Health Canadas Environmental and Occupational Toxicology Division. 'We have known that mothers who smoke can harm their fetuses, and here w...

Drug Used to Prevent HIV Transmission from Mother to Child Damages DNA

...nstability may be the contributing factors to the pattern of mutations observed in the study, according to the authors. They conclude, "The cumulative mutagenesis data suggest that infants exposed transplacentally to AZT may be at increased risk for cancer as they age." In the first of the two human studie...

Drug Used to Prevent HIV Transmission from Mother to Child Damages DNA

...e published in April 2007 in a special issue of Environmental and Molecular mutagenesis that examines the latest research on DNA damage and potential health risks ...rved in the study, according to the authors. They conclude, "The cumulative mutagenesis data suggest that infants exposed transplacentally to AZT may be at increas...

Elusive Step In DNA Mutation Uncovered By Researchers

...chain of events to an abnormally high rate of cell division – the result is cancer. "We set out to explore the elementary processes that lead to mutagenesis and eventually cancer," said Uzi Landman, director of the Center for Computational Materials Science and Regents' professor and Callaway chair of phys...

Depression May Be A Risk Factor For Cancer Initiation

...he base modifications due to oxidative stress is 8-hydroxydeoxyguanosine (8-OH-dG). It has been reported that this oxidative DNA damage indicates both mutagenesis and carcinogenesis, and has been considered to be useful in estimating cancer risk. Owing to these findings, the formation of 8-OH-dG seems to be a va...
Mutagenesis in Biological News

Structural biology scores with protein snapshot

...he active site looks nothing like any other kinase active site it's a unique architecture," Sanders said. The researchers also performed exhaustive mutagenesis studies in which they characterized mutations at each amino acid in DAGK and used the data to map the active site of the enzyme onto the structure. Th...

Forced evolution: Can we mutate viruses to death?

...er values will lead to this delocalization. A competing theory is that a mutagenesis drug may eradicate a virus or bacterial population by reducing the fitness ...like the new model, drug designers looking to create pills to induce lethal mutagenesis couldn't say for certain under what parameter ranges the drugs really worke...

The 21st century tomato

...toes for size, shape, texture, flavor, shelf-life, and nutrient composition, more or less, one trait at a time. With the advent of molecular biology, mutagenesis and genetic transformation could produce tomatoes that were more easily harvested or transported or turned into tomato paste. Frequently, however, op...

Biomarkers allow doctors to match therapy to patient

...be regulated at a molecular level by specific genes. To identify these genes involved in cell growth, they used a functional screen based on insertion mutagenesis with mouse retroviruses. This unique approach in human solid tumor cells took more than a decade of painstaking work. Some of the seven genes the ...

Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution

...bor Protocols ( www.cshprotocols.org/TOCs/toc4_08.dtl ) highlights a method for screening the mouse genome using ENU mutagenesis. The method, "Mouse mutagenesis Using N -ethyl- N -nitrosourea (ENU)," was submitted by Monica Justice and colleagues from the Baylor College of Medicine http://www.bcm.edu/db/db_f...

Scientists unravel the genetic coding of the pea

...s for both basic science and for crop improvement. TILLING is an alternative to Agrobacterium-based techniques, and uses EMS (ethane methyl sulfonate) mutagenesis coupled with a gene-specific detection of single-nucleotide mutations. This reverse genetic strategy can be applied to all types of organisms and can ...

Environmental epigenetics has potential for preventing and treating disease

...expression that are inherited without changing the sequence of DNA. An article publishing in the January 2008 issue of Environmental and Molecular mutagenesis examines some of the epigenetic mechanisms linked to disease, and explores how they occur and their significance in understanding, treating and preven...

Biophysical Society announces winners of 2008 Minority Travel Awards

...ynaptotagmin I that Contributes to Differential Transmitter Release. Kerrick, Nevels, University of Maryland School of Pharmacy, Site-Directed mutagenesis of the Bt Toxin Cyt1A for Intramolecular Distance Determination by FRET. Joseph Sabat, Albert Einstein College of Medicine, Proximal Ligand Swi...

'Jumping genes' could make for safer gene delivery system

...Transposons, or jumping genes, argues Lambert, are a potentially safer way to go. "You lose the infectivity component and you minimize the insertional mutagenesis risk." Techniques for targeting transposon vectors to regions of the genome devoid of cancer genes are being refined. Meanwhile, a key advantage ov...

Cigarette smoke alters DNA in sperm, genetic damage could pass to offspring

...ist as irreversible changes in the genetic composition of off-spring.” said Carole Yauk, Ph.D., lead author of the study and research scientist in the mutagenesis Section of Health Canada’s Environmental and Occupational Toxicology Division. “We have known that mothers who smoke can harm their fetuses, and here ...
Mutagenesis in Biological Technology

Randomize Gene Sequences with New PCR Mutagenesis Kit

GeneMorph PCR mutagenesis kit produces a unique mutational spectrum Janice Cline Holly Hogrefe Stratagene ...

High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors

QuikChange XL: a highly versatile kit for site-directed mutagenesis of large plasmids Michael Borns Peter Vaillancourt Holly Hogrefe Jeff Braman Stratagene The QuikChange site-directed mutagenesis kit* characteristically generates mutagenesis efficiencies exceeding 80%...

Big Blue Rodent Cell Cultures for Mutagenesis Studies

Rat and mouse embryonic cell lines are useful for in vitro mutagenesis studies Denise L. Wyborski Stratagene Cloning Systems, Inc. The Big Blue rat cell line and the Big Blue transgenic mouse embryonic cell line are useful for in vitro mutagenesis studies. These cell lines are ...

Frost & Sullivan Recognizes Cisbio Bioassays for Technology Innovation

...e technologies. "The determination of ligand binding affinity has been used to delineate the ligand binding site of GPCRs through a site-directed mutagenesis strategy," says Frost & Sullivan's Dr. Sudeep Basu. "These studies also provide clues to the activation mechanism of the receptors." It is in...

Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market

...is an amalgamation of core enzyme catalysis, high-specificity DNA targeting, computational molecular simulations for structural modeling, and rational mutagenesis to design a set of extremely specific 'molecular scissors' for the precise engineering of large genomes." The DNE technology is so specific that ...

The New Green Revolution offers hope to poor farmers and poor consumers

...ty to develop insect-resistant potatoes and sweet potatoes. An Austrian lab is working with researchers in Kenya to transfer technology on cassava mutagenesis and breeding. Cassava is a low-protein, starchy staple in regions where cereal grains cannot be easily grown. Monsanto, Pioneer, Astra-Zeneca and U...

Detection of Mutations in the CYP21 Gene Using the DCode System

... the sequence of interest. Site-Directed mutagenesis In order to obtain mutant control sam...he mutant controls were generated by site-directed mutagenesis at the following nucleotide positions: ...d de Vos, W. M., Improved site-directed mutagenesis method using PCR, Nucleic Acids Res., 19 (16):4558...

Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System

...er, C.S., Eichholtz, D.A., Frazier, R.B., Hironaka, C.M., Levine, E.B., Shah, D.M., Fraley, R.T. and Kishore, G.M. Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. Journal of Biological Chemistry 266(33):22364-22369 ...

A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis

...f mutations was created by site directed mutagenesis in an exon 4 containing clone. The indiv... Materials and Methods SITE-DIRECTED mutagenesis OF hMSH2 EXON 4 A series of mutations...he primer resulted in the site-directed mutagenesis of the amplified product. As the mutage...

Enjoy a Greater Sense of Security with PCR-Grade Water

...asks to be addressed by the molecular biology field. In order to assign functions to the genes encoded in the enormous amounts of sequence data, mutagenesis methods are of paramount importance. By combining a powerful polymerase chain reaction (PCR) technique to generate linear expression fragments w...
Mutagenesis in Biological Products

GeneTailor Site-Directed Mutagenesis System from Invitrogen

Description: ...
Company:Invitrogen

AMAP Multi Site-directed Mutagenesis Kit from MBL International

Description: AMAP Multi Site-directed Mutagenesis Kit Research Focus: other Storage Temperature: -20C Shipping Temperature: dry ice...
Company:MBL International

Site-directed DNA Mutagenesis Service from Yorkshire Bioscience Ltd

Description: Deletions, insertions or substitutions. Mutations in plasmids of size up to 15 kb. Single or multiple mutations...
Company:Yorkshire Bioscience Ltd

Site-directed Mutagenesis from Exon BioSystems

Description: Any form, any size, any number of mutageneses. ExonBio provides the best service with double sequecing to make sure the success of the mutagenesis....
Company:Exon BioSystems

GPS -M Mutagenesis System from New England Biolabs

Description: The GPS-M Mutagenesis System enables you to insert a transposable element of your own design into your target DNA by in vitro transposition. It uses TnsABC* Transposase to insert a Tn7-based transposon randomly into the DNA target. Target DNA may be a plasmid, cosmid, BAC or purified chromoso...
Company:New England Biolabs

Random DNA mutagenesis Service from Yorkshire Bioscience Ltd

Description: We can introduce random mutations to the gene supplied by a customer. Mutated DNA will be then cloned into a vector of choice to obtain a library of the mutated DNA. Mutation frequency can be set to a desired value within 1-20 mutations per kb. Number of clones 106-108. Mutations can be restrict...
Company:Yorkshire Bioscience Ltd

Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent) from Promega

Description:...thesized unmethylated strand (1), leading to high mutation efficiencies and making them helpful in such systems as the GeneEditor and Altered Sites II mutagenesis Systems. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is used in the Alt...
Company:Promega

Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent) from Promega

Description:...thesized unmethylated strand (1), leading to high mutation efficiencies and making them helpful in such systems as the GeneEditor and Altered Sites II mutagenesis Systems. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is used in the Alt...
Company:Promega

MUTAN SUPER EXPRESS KM 20 RXN from Takara Mirus Bio

Description:... Takara's Mutan-Super Express Km Kit is designed to achieve site-directed mutagenesis in just one day. It is based on Oligonucleotide-directed Dual Amber (ODA) method, using the advantages of LA (Long and Accurate) PCR....
Company:Takara Mirus Bio

GCpro Taq DNA Polymerase Complete System from GenScript Corporation

Description:...rd PCR application. It is versatile, user-friendly and compatible with all PCR applications such as GC-rich and other complex templates, site-directed mutagenesis as well as TA cloning due to its extendase activity....
Company:GenScript Corporation

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Mutagenesis in Biological Definition

Ion channel

...archers have deduced about channel operation so far they have established through electrophysiology , biochemistry , gene sequence comparison and mutagenesis . History The existence of ion channels was hypothesized by the British biophysicists Alan Hodgkin and Andrew Huxley as part of their ...

Polymerase chain reaction

...of hereditary diseases 4.4 Cloning genes 4.5 mutagenesis 4.6 Analysis of ancient DNA 4.7 Genotyping o...y from organism A, occurring in organism B. mutagenesis mutagenesis is a way of making changes to the sequence of nu...

Reverse genetics

...ence a sequence has on phenotype, or to discover its biological function, researchers may engineer a change or disruption in it -- by site-directed mutagenesis , for example, by deletion of a gene by gene knockout (as can be done in some organisms, such as yeast and mice ) -- and only afterwards look for t...
Mutagenesis in Biological Dictionary

Mutagenesis

Mutagenesis . From Wikipedia, the free encyclopedia. Jump to: navigation, search ... Retrieved from "http://en.wikipedia.org /wiki/ Mutagenesis " Categories: Mutation ... Sequencing, Mutagenesis & Labeling ... Site-directed mutagenesis is a cornerstone technique in mol...

Saturation mutagenesis

Saturation mutagenesis is a simple method that can be used to scan a promoter ... Simultaneous saturation mutagenesis of multiple nucleotides can yield an ... Saturation mutagenesis is a simple method that can ... saturation mutagenesis to address a ... Simultaneous saturation...

Transversion

... the ... TransVersion (Description: Discuss topics of TransVersion ) - this ... ... efficiently extended implicating O2-Et-dT in transversion mutagenesis in vivo. ... of the oncogene neu by an A.T----T.A transversion event in rat neuroblastomas. ... The promoter sequence having this transve...

Transposon

...etic ... Knock Out ... SB, like other DNA transposons , mobilizes itself via a cut-and ... The SB transposon is a powerful tool for insertional mutagenesis in many ... The existence of transposons was postulated in the late 1940s by Barbara ... [4] The transposon codes for antibiotic resistance ...

Pseudorevertant

...was able to grow on maltose. ... Combinatorial mutagenesis and pseudorevertant analysis to characterize re...gions ... have been investigated by combinatorial mutagenesis and pseudorevertant mapping. ... Introductio...eudorevertant (data not shown) ... Combinatorial mutagenesis and pseudorevertant analysis to characterize re...

P elements

... A P element is a transposon that is present in the fruit fly Drosophila melanogaster and is used widely for mutagenesis and the creation of genetically modified flies used for genetic research. The P element gives rise to a... Phosphorus is the chemical element th...

Oligonucleotide

...r ... An oligonucleotide , a short stretch... One method of in vitro mutagenesis is oligonucleotide -directed mutagenesis. ... This oligonucleotide is h...eotide from Biology-Online.org dictionary. ... Oligonucleotide directed mutagenesis ... ...

Mutagen

...cificity (genetics) spontaneous ... Entry in the Material Safety Data Sheet HyperGlossary at ILPI. ... The Environmental Mutagen Society studies mutagenesis and its applications to ... MySpace Music profile for MuTaGeN . Download MuTaGeN Psychedelic / Progressive / Trance music singles, watch music ...

Intergenic region

...1, the conserved intergenic regions may be important for transcription ... ... (3'-GA-5') intergenic region , the NJ variant showed ... 5. mutagenesis of the 21-nucleotide G-L intergenic region from VSV New Jersey, Ogden strain. ... Footprints cover 20% of intergenic regions , often occur i...

Genomic clone

...xample, when a genomic clone is isolated from information about its mRNA ... Another 'easy' way to identify a genomic clone is by insertional mutagenesis ... Large Insert Clone / Genomic Sequencing Approaches. Viral Vector. Molecular Genetics and Genomics ... All genomic libraries are construc...
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