Mutagen

GlaxoSmithKline Obtains Exclusive U.S. OTC Marketing Rights to MEVACOR(R) (lovastatin) from Merck & Co., Inc.

...a gland of the eye of rodents) were significantly higher in high dose mice than in controls. No evidence of mutagenicity was observed in a microbial mutagen test using mutant strains of Salmonella typhimurium with or without rat or mouse liver metabolic activation. In addition, no evidence of damage to gen...

Drug Used to Prevent HIV Transmission from Mother to Child Damages DNA

...t findings on the toxicity of these drugs toward mitochondrial DNA. Environmental and Molecular Mutagenesis, the official journal of the Environmental mutagen Society, is published by John Wiley & Sons, Inc. and is available online via Wiley InterScience at (http://www.interscience.wiley.com/journal/emm). ...

Skin Cancer Risk Increases With Ultraviolet-B Radiation Exposure

...he number of chromatid breaks increased so did the risk of developing skin cancer, but not melanoma. "These findings suggest that in vitro UVB-induced mutagen sensitivity reflects susceptibility to nonmelanoma skin cancer but not cutaneous malignant melanoma," the authors concluded. Contact Ariel Whi...

Mutagen in Biological News

Munich researchers discover new target for tailored antibiotics

... of the active site cavity. The crystal structure opened the door to a detailed examination of the reaction mechanism using computer simulation and mutagen experiments, in which E. coli bacteria are coerced into synthesizing defective IspH enzymes. Thus, the X-ray structure, kinetic measurements, and mu...

Environmental epigenetics has potential for preventing and treating disease

...eir significance in understanding, treating and preventing disease. Environmental and Molecular Mutagenesis, the official journal of the Environmental mutagen Society, is published by John Wiley & Sons, Inc. and is available online via Wiley InterScience at http://www3.interscience.wiley.com/cgi-bin/jissue/...

New technique could alter field of mouse genetics

...n acts as a genetic beacon, so researchers can easily track its location without having to sequence the entire genome, as can happen with the chemical mutagen technique. In three months, the two graduate students who led the project, generated knockout mice for each of 75 different genes. Xu, Min Han, an ...

Breast-Cancer Risk Linked to Exposure to Traffic Emissions at Menarche, First Birth

...d tests on individual vehicles. A geographic model was used to reconstruct historic traffic PAHs, using measurements of benzo[a]pyrene, a known potent mutagen and carcinogen, as a surrogate for total PAH exposure. Cruise emissions, cold engine emissions and intersection emissions were used to estimate total ...

Mutagen in Biological Definition

Mutagen

In biology , a mutagen ( Latin , literally origin of change ) is an agent that changes the genetic information (usually DNA ) of an organism and thus increases the number of mutations above the natural background level. Mutagens are usually chemical compounds or radiation . The A...

Ames test

...wn" of bacteria is present. The experimental cultures are exposed to the agent to be tested while the positive control cultures are exposed to a known mutagen to confirm that there has been no contamination of the strain. Strains of bacteria are available which have been genetically modified such that only a...

Molecular genetics

...le to molecular geneticists is the forward genetic screen . The aim of this technique is to identify mutations that produce a certain phenotype . A mutagen is very often used to accelerate this process. Once mutants have been isolated, the mutated gene can be molecularly identified. Reverse gene...

Mutagen in Biological Dictionary

Mutagen

In biology, a mutagen is a physical or chemical agent that changes the genetic material (usually DNA) of an organism and thus increases the frequency of mutations above the natural background level. As many mutations cause cancer, mutagens are typically also... mutagen ( ) n. An agent, suc...

Sub-cloning

...xistent restriction sites and tedious sub - cloning procedures make it ... method for cloning protein domain and sub -domains after random mutagen ... The solution from the cuvette is diluted and then plated on a large 9x9-inch ... This is incubated at 37°C for 18-20 hours. Only those cells ...

Gene knockout

...e gene modification): knockout mouse (KO mouse), knock ... ... Gene Knockout ... The gene knockout strategy involves using a random mutagen ... produce a small deletion (100-1000 bp) in any gene of interest. ... ...

Ethidium Bromide

...released to the drain. ... Follow these procedures for working with ethidium bromide safely. ... Ethidium bromide (C21H20BrN3) is a potent mutagen used as a nucleic acid stain. ... Safety shower and eye wash should be easily accessible where ethidium bromide is used. ... Trace amounts o...

Ames test

...nd professor of ... The Ames test uses a mutant strain of Salmonella typhimurium which cannot grow ... By definition in the Ames test , a mutagen is any chemical agent which induces ... The spot-overlay Ames test of potential mutagens allows us to address ... mind, Bruce Ames and his c...