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MutS in Biological News

Enzyme crystal structure reveals 'unexpected' genome repair functions

...ere surprised when we found that XPB contains a domain structurally similar to the mismatch recognition domain of a bacterial DNA repair protein MutS. muts helps recognize and repair mismatched DNA in E. coli. These two proteins have little sequence similarity. Biochemical assays following this discovery ...
MutS in Biological Technology

140-Fold Increase of Protein Achieved in Pichia Fermentation

...xpressly written for Pichia pastoris to BioFlo 3000 users upon request. Materials & Methods In these studies, a muts strain of Pichia was grown in fed-batch and continuous culture. AFS BioCommand helped to control and later optimize nutrient feeding and oxygen ...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

...d mismatch repair genes have also been identified. These genes include mMSH2, 13 mPMS2 14 and GTMBP 15 (mGTBP). Rep-3 in mice is similar to the muts gene but shares the greatest homology to yeast MSH3. 16 Rapid advances in the field of DNA repair have begun to elucidate the precise role of...
MutS in Biological Products

Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent) from Promega

Description:... BMH 71-18 muts and ES1301 muts are mismatch repair minus strains of E. coli. Use ... II Mutagenesis Systems. Both ES1301 and BMH 71-18 muts are recA+, therefore inserts containing highly rep...
Company:Promega

Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent) from Promega

Description:... BMH 71-18 muts and ES1301 muts are mismatch repair minus strains of E. coli. Use ... II Mutagenesis Systems. Both ES1301 and BMH 71-18 muts are recA+, therefore inserts containing highly rep...
Company:Promega
MutS in Biological Dictionary

MutS

MutS forms a dimer (MutS2) that recognises the mismatched base on the daughter ... The crystal structure of MutS reveals that it is exceptionally asymmetric, and ... MutS protein initiates mismatch repair with recognition of a non-Watson-Crick ... Integration of these MutS D...

Transversion

... a ... With the same transversion from the blaOXY-2 gene only a fivefold increase was ... Conversely, GC TA transversion increased in mutL or muts mutants in stationary phase. ... The rate of lacZ GC TA transversion was determined by the method of Lea and ... 2. Which of the substitutions in...

Post-meiotic segregation

...ll ... increase in post - meiotic segregation is a feature of ... post - meiotic ... Post - meiotic segregation . Meiotic abnormalities. muts homologs. Msh2. No ... have an increase in post - meiotic segregation (PMS), a term ... post - meiotic segregation , orthologous to yeast PM...

Mutagenesis

...ite-directed mutagenesis is a cornerstone technique in molecular biology, with ... II in vitro Mutagenesis Systems ... Mutagenesis Systems. muts Competent Cells. Nested Deletion Mutagenesis System ... Altered Sites® II Mammalian Mutagenesis Systems. 426kb. TM047 ... Human Molecular Genet...

MSH2

...dule and regulate two branches of the damage ... ... homologs form two major heterodimers: Msh2 /Msh6 (MutSα) and Msh2 /Msh3 (MutSβ) ... the muts and MutL homologues MSH2 and MLH1 respectively, which allows them ... The human mutator gene homolog MSH2 and its association with hered...

Heteroduplex Dna

...s. Double stranded DNA is not a completely straight rigid rod. ... ... muts protein to a heteroduplex DNA having a single unpaired thymidine ... ...A mixtures are amplified by PCR for heteroduplex detection, ... ... a muts dimer and a DNA heteroduplex are converted ... unmodified strand of...
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