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Microsatellite in Medical News

A breakthrough in gastric carcinogenesis

...hylation status of the promoter region of CHFR and microsatellite instability (MIN) status in primary gastric cancer...astric cancer using methylation-specific PCR. Five microsatellite loci were analyzed using high-intensity microsatellite analysis reported previously, and p53 gene mutatio...

LOH analysis on 4q in sporadic colorectal carcinoma

... Thirteen fluorescent labeled polymorphic microsatellite markers were analyzed in 83 cases of colorectal carcinoma and matched normal tissue DNA by polymerase chain reaction (PCR). PCR products were eletroph...

Does 1q31.1-32.1 harbor a tumor suppressor gene related to Chinese SCC patients?

... The LOH analysis on sporadic carcinoma by means of microsatellite markers has become an effective way to find allelic deletion regions and th...ing and functional study. Therefore, further LOH scanning with high-density microsatellite markers in the region was found to be necessary to narrow the research scop...

Scientists explore factors contributing to DNA mutations

...ilarly, conservation geneticists can use a lack of microsatellite variability among individuals in a group to distin...tifying the most important factors contributing to microsatellite mutability -- the microsatellite's ability to muta...icating that individual factors might be affecting microsatellite mutability, but nobody has looked at how these fac...

New Test May Better Identify Certain Colorectal Cancer Types

...hese mutations by looking for a gene marker called microsatellite instability, which is caused by those gene defects...on-hereditary form of colorectal cancer that shows microsatellite instability tend to have a better prognosis than o... experimental method, of identifying patients with microsatellite instability. They found that the experimental meth...

Aspirin and other NSAIDS Proved Less Effective in Long-Term Smokers

...ay be associated with smoking. Such tumors display microsatellite instability, an acquired genetic characteristic that indicates defects in DNA-repair machinery. microsatellite instability, or MSI, occurs in approximately 15 pe...rin and other NSAID use, among other risk factors. microsatellite instability was assessed in tumors from 1,202 case...

Skin tests confirms cancer

...they do not they can cause alterations to microsatellites - the short repetitive sequences of genetic code contained in DNA. This phenomenon, known as microsatellite instability (MSI), can cause tumours to develop. Dr Bataille and her team inspected skin lesions of families with and without a history of hered...
Microsatellite in Medical Products

Applied Biosystems 3730 DNA Analyzer

Description:...ur productivity without compromising your results. Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation. ...
Company:Applied Biosystems

Applied Biosystems 3730xl DNA Analyzer

Description:...ur productivity without compromising your results. Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation. ...
Company:Applied Biosystems

CEQ 8000 Genetic Analysis System

Features:... De novo DNA Sequencing. Heterozygote detection. Confirmatory sequencing. Mutation analysis. Allele identification. SNP scoring. microsatellite instability. AFLP fingerprinting. Gene expression....
Company:Beckman Coulter, Inc.

CEQ™ 8000 Genetic Analysis System

Features:... De novo DNA Sequencing. Heterozygote detection. Confirmatory sequencing. Mutation analysis. Allele identification. SNP scoring. microsatellite instability. AFLP fingerprinting. Gene expression....
Company:Beckman Coulter, Inc.
Microsatellite in Biological News

T-STAR grant funds genetic studies on shrimp and papaya and endangered cycad rescue

...for a genetic variability study of specific pathogen free (SPF) Pacific white shrimp, Penaeus vannamei, through developing a panel of species-specific microsatellite genetic markers. Says Dr. Gong, "The long term research objective is to establish a medium-scale, effective genetic selection program of P. vannamei o...

Land iguanas under continuing threat on Galapagos archipelago

...ty within and relationships among populations are crucial for long-term species survival, the authors investigated genetic variation at nine nuclear microsatellite loci among more than 700 land iguanas from six island habitats. For comparison, the information obtained was compared with similar information gathere...

The good, the bad and the smelly: USGS at the 2008 Coral Reef Symposium

...wii. A. sydowii is a globally distributed fungus commonly found in soil, so its presence in marine systems raises questions about its origin. By using microsatellite markers, researchers analyzed the population structure of A. sydowii from diseased sea fans, diseased humans and environmental sources worldwide. The ...

To fight the cancer before the tumor grows

...the ability to express the foreign proteins on their surface. The results of the Heidelberg research team suggest that the growth of tumous with microsatellite changes can be prevented if the immune system can be activated against the foreign proteins in time. The researchers next goal is thus to develop a va...

Concentrating on different aspects of pain leads to breakthrough in migraine genetics

...families had been ascertained through neurology clinics, while the Australian families had been collected through a twin study. An initial genome-wide microsatellite study was followed up by an independent targeted replication study. Researchers identified one gene locus on chromosome 10q23, which showed signif...

Global climate change: The impact of El Nio on Galpagos marine iguanas

...he researchers looked for changes in levels of genetic variation in nuclear microsatellite DNA and mitochondrial DNA markers of samples collected before and after this El Nino event. Changes in microsatellite frequency are sensitive enough to detect low population sizes before there ...

Global climate change: The impact of El Niño on Galápagos marine iguanas

...he researchers looked for changes in levels of genetic variation in nuclear microsatellite DNA and mitochondrial DNA markers of samples collected before and after this El Nino event. Changes in microsatellite frequency are sensitive enough to detect low population sizes before there ...

UW launches cutting-edge DNA 'fin-printing' project for salmon

...of the chemicals that make up the chains of DNA in animals. This makes using SNPS simpler and easier to standardize than other genetic markers such as microsatellite markers that involve longer portions of DNA. The UW School of Aquatic and Fishery Sciences already has created a large database on the ranges of hig...

Does the desire to consume alcohol and tobacco come from our genetic makeup?

...nnaires. The researchers used a dense map (three haplotypes per cM; r2>0.4), generated by merging 58000 SNPs (single nucleotide polymorphisms) and 437 microsatellite markers, to identify sex-specific and non-specific linked and associated areas. Summary of Results The researchers reported the following resul...

Genetic analysis of cavefish reveals more about evolution

...e researchers examined two distinct cave-dwelling fish populations. To provide a framework in which to study the genetics of this species, they made a microsatellite linkage map, which allowed them to locate the regions of the chromosomes that had genes controlling cave related traits. The researchers found that...
Microsatellite in Biological Technology

Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci

Marion Schmidt, Holger Engel, Markus Sprenger-Hauels, Corinna Kppers, Gaby Schulte, Silke Zobel, and Dirk Lffert QIAGEN GmbH, Hilden, Germany Microsatellites highly polymorphic DNA markers comprised of nucleotides ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

...004_Feature_Article.pdf ). 18. C. Brueck, personal communication. 19.Grinzinger D., et al., (2000) Measurement of DNA copy number at microsatellite loci using quantitative PCR analysis. Cancer Res. 60 : 5405-9. Extract-N-Amp is a trademark of Sigma-Aldrich &#...

Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies

...rived DNA to study genetic alterations and their relationship to human diseases. It has become a useful tool for many biomedical applications, such as microsatellite analysis, single nucleotide polymorphism (SNP) detection, comparative genomic hybridization (CGH) microarrays and others. GenomePlex...

SEESNP DISCOVERY

...prietary software packages. PolyPhred sequence analysis services are available to validate SNPs and identify insert/deletion and microsatellite repeats. ...

Custom and library siRNA for efficient gene silencing

...and plants, detection of bacteria and viruses, and microsatellite analyses Easy to use and cost-effective ...r complex secondary structure. microsatellite Analysis with Optimized QIAGEN Protocol ... Figure 2 Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51, and P...

Custom and library siRNA for efficient gene silencing

... Versatile for many applications - including typing of transgenic animals and plants, detection of bacteria and viruses, and microsatellite analyses Easy to use and cost-effective - simple reaction setup for fast and reproducible resu...

QIAGEN Multiplex PCR Kit

...ion and analysis of microsatellites (see figure: " microsatellite Analysis with Optimized QIAGEN Protoc... microsatellite Analysis with Optimized QIAGEN Protocol ... Analysis of microsatellite loci D3S1358, TH01, D21S11, D18S51,...

HotStarTaq PCR Handbook

...tion. The kit is highly suited for multiplex PCR applications such as typing of genetically modified animals and plants, microsatellite analysis, determination of bacteria and viruses, or amplification of regions carrying SNPs. For more information about t...

QIAGEN Multiplex PCR Handbook

... Multiplex PCR Protocol for Amplification of microsatellite Loci Multiplex PCR...ically modified animals and plants, microsatellite analysis, determination of bacteria and viruses, ...iplex PCR Protocol for Amplification of microsatellite Loci Important notes before starting ...

RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes

... DNA mismatch repair. In addition to correcting single base mispairing, mismatch repair pathways have been suspected to play a role in controlling microsatellite instability in proto-oncogenes 2 and in modulating adaptive mutation in nondividing cells. 3 The characterization of the E. coli MutHLS mismat...
Microsatellite in Biological Products

SagaGT Microsatellite Analysis Software (Server edition) from LI-COR Biosciences

Description: For microsatellite research, the 4300 System features Saga GT , is an advanced genotyping automation software available. SagaGT automates electrophoresis, lane finding, location of standards, calibration of band sizes, and allele scoring. Saga GT is second generation software developed by LI-COR...
Company:LI-COR Biosciences

CEQ 8800 Genetic Analysis System from Beckman Coulter

Description:...A sequencing, heterozygote detection, confirmatory sequencing, mutation analysis, allele identification, SNP (single nucleotide polymorphism) scoring, microsatellite instability and amplified fragment length polymorphism fingerprinting. To ensure accurate sample tracking, CEQ 8800 sample set-up can be created at t...
Company:Beckman Coulter
Microsatellite in Biological Definition

Microsatellite

This article is about the DNA sequence. See miniaturized satellites for orbiting artificial satellites with a mass of less than 100Kg. A microsatellite is a short, noncoding DNA sequence (a Tandemly Repetitive DNA sequence) that is repeated many times within the genome of an organ...

Repeated sequence (DNA)

...he study of DNA sequences, one can distinguish two main types of repeated sequence : Tandem repeats : Satellite DNA , Minisatellite , microsatellite ; Interspersed repeats : SINE ( S hort IN terspersed E lements), LINE ( L ong IN terspersed E lements). ...
Microsatellite in Biological Dictionary

Microsatellite

2.1 Development of microsatellite primers. 2.2 ISSR-PCR. 3 ... One common example of a microsatellite is a (CA)n repeat, where n is variable between alleles. ... 1.2 Microsatellite . 1.3 Nanosatellite. 1.4 Picosatellite. 2 Technical challenges. 3 See also ... example of a low-cost m...

Polymorphic loci

... loci shown in FIGS. ... Selectively Amplified microsatellite Polymorphic Loci . Start using this tool. Selective amplified microsatellite polymorphic loci (SAMPL) analysis (Witsenboer...in ... Abstract: We developed 15 polymorphic microsatellite loci from the Hawaiian tree ... These loci we...

Intragenic

...almonella typhimurium: ... OBJECTIVE: Analysis of the intragenic SHOX microsatellite to define its utility ... CONCLUSIONS: The SHOX intragenic microsatellite might be a useful molecular ... Intragenic deletions and duplications...

Insertion mutations

... In genetics, an insertion is the addition of one or more nucleotide base pairs into a DNA sequence. This can often happen in microsatellite regions due to the DNA polymerase slipping. Insertions can be anywhere in size from one base pair incorrectly inserted into a DNA... In biolog...

Gene insertion

... In genetics, an insertion is the addition of one or more nucleotide base pairs into a DNA sequence. This can often happen in microsatellite regions due to the DNA polymerase slipping. Insertions can be anywhere in size from one base pair incorrectly inserted into a DNA... The c-myc...

Flanking region

...g region from one virus with the counterpart ... ISHS IX International Conference on Grape Genetics and Breeding CHARACTERIZATION OF SNPS FROM microsatellite FLANKING REGIONS IN VITIS Exon 1: 13 sequences from 6 species with flanking regions and splice-typing . The 5'- flanking region of the...

CpDNA

... population expansion is suggested for both C. ... posted to cpdna gene-flow mtdna phylogeography plateau postglacial ... posted to cpdna microsatellite parallel-evolution plant population-genetics by ... CiteULike is a free online bibliography manager. Register and you can start organising ... L...
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